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. 1982 Jul 1;156(1):104–111. doi: 10.1084/jem.156.1.104

Direct demonstration of an HLA-DR allotypic determinant on the low molecular weight (beta) subunit using a mouse monoclonal antibody specific for DR3

JP Johnson, T Meo, G Reithmuller, DJ Schendel, R Wank
PMCID: PMC2186719  PMID: 6177816

Abstract

A murine monoclonal antibody directed against a human B cell surface antigen with the characteristics of HLA-DR is described. The antigen detected is tightly linked to HLA and is correlated with the alloantigen HLA-Dw/DR3. Reactivity with a fraction of Dw/DRw6 cells is also observed. The determinant recognized by this antibody has been shown to be present on the smaller molecular weight β subunit of the HLA-DR antigen.

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Selected References

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  1. Barnstable C. J., Jones E. A., Bodmer W. F., Bodmer J. G., Arce-Gomez B., Snary D., Crumpton M. J. Genetics and serology of HL-A-linked human Ia antigens. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):443–455. doi: 10.1101/sqb.1977.041.01.052. [DOI] [PubMed] [Google Scholar]
  2. Bergholtz B. O., Thorsby E. HLA-D restriction of the macrophage-dependent response of immune human T lymphocytes to PPD in vitro: inhibition by anti-HLA-DR antisera. Scand J Immunol. 1978;8(1):63–73. doi: 10.1111/j.1365-3083.1978.tb00496.x. [DOI] [PubMed] [Google Scholar]
  3. Brodsky F. M., Parham P., Bodmer W. F. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens. 1980 Jul;16(1):30–48. doi: 10.1111/j.1399-0039.1980.tb00285.x. [DOI] [PubMed] [Google Scholar]
  4. Carrel S., Tosi R., Gross N., Tanigaki N., Carmagnola A. L., Accolla R. S. Subsets of human Ia-like molecules defined by monoclonal antibodies. Mol Immunol. 1981 May;18(5):403–411. doi: 10.1016/0161-5890(81)90102-4. [DOI] [PubMed] [Google Scholar]
  5. Charron D. J., McDevitt H. O. Characterization of HLA-D-region antigens by two-dimensional gel electrophoresis. Molecular-genotyping. J Exp Med. 1980 Aug 1;152(2 Pt 2):18s–36s. [PubMed] [Google Scholar]
  6. Johnson J. P., Demmer-Dieckmann M., Meo T., Hadam M. R., Riethmüller G. Surface antigens of human melanoma cells defined by monoclonal antibodies. I. Biochemical characterization of two antigens found on cell lines and fresh tumors of diverse tissue origin. Eur J Immunol. 1981 Oct;11(10):825–831. doi: 10.1002/eji.1830111015. [DOI] [PubMed] [Google Scholar]
  7. Kaufman J. F., Andersen R. L., Strominger J. L. HLA-DR antigens have polymorphic light chains and invariant heavy chains as assessed by lysine-containing tryptic peptide analysis. J Exp Med. 1980 Aug 1;152(2 Pt 2):37s–53s. [PubMed] [Google Scholar]
  8. Klareskog L., Rask L., Fohlman J., Peterson P. A. Heavy HLA-DR (Ia) antigen chain is controlled by the MHC region. Nature. 1978 Oct 26;275(5682):762–764. doi: 10.1038/275762a0. [DOI] [PubMed] [Google Scholar]
  9. Lampson L. A., Levy R. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980 Jul;125(1):293–299. [PubMed] [Google Scholar]
  10. MORTON N. E. Sequential tests for the detection of linkage. Am J Hum Genet. 1955 Sep;7(3):277–318. [PMC free article] [PubMed] [Google Scholar]
  11. Markert M. L., Cresswell P. Polymorphism of human B-cell alloantigens: evidence for three loci within the HLA system. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6101–6104. doi: 10.1073/pnas.77.10.6101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nadler L. M., Stashenko P., Hardy R., Tomaselli K. J., Yunis E. J., Schlossman S. F., Pesando J. M. Monoclonal antibody identifies a new Ia-like (p29,34) polymorphic system linked to the HLA-D/DR region. Nature. 1981 Apr 16;290(5807):591–593. doi: 10.1038/290591a0. [DOI] [PubMed] [Google Scholar]
  13. Shackelford D. A., Strominger J. L. Demonstration of structural polymorphism among HLA-DR light chains by two-dimensional gel electrophoresis. J Exp Med. 1980 Jan 1;151(1):144–165. doi: 10.1084/jem.151.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Silver J., Ferrone S. Structural polymorphism of human DR antigens. Nature. 1979 May 31;279(5712):436–437. doi: 10.1038/279436a0. [DOI] [PubMed] [Google Scholar]
  15. Tosi R., Tanigaki N., Centis D., Ferrara G. B., Pressman D. Immunological dissection of human Ia molecules. J Exp Med. 1978 Dec 1;148(6):1592–1611. doi: 10.1084/jem.148.6.1592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Trucco M. M., Garotta G., Stocker J. W., Ceppellini R. Murine monoclonal antibodies against HLA structures. Immunol Rev. 1979;47:219–252. doi: 10.1111/j.1600-065x.1979.tb00295.x. [DOI] [PubMed] [Google Scholar]
  18. Williams A. F. Assays for cellular antigens in the presence of detergents. Eur J Immunol. 1973 Oct;3(10):628–632. doi: 10.1002/eji.1830031007. [DOI] [PubMed] [Google Scholar]
  19. Zola H., Bradley J., Macardle P., McEvoy R., Thomas M. A hybridoma antibody which reacts with human cells carrying the DRw4 tissue type. Evidence for heterogeneity of DRw4. Transplantation. 1980;29(1):72–73. [PubMed] [Google Scholar]

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