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. 1978 Oct;136(1):414–418. doi: 10.1128/jb.136.1.414-418.1978

Survey of extrachromosomal DNA found in the filamentous cyanobacteria.

R D Simon
PMCID: PMC218673  PMID: 101520

Abstract

Cleared lysates of 13 species of filamentous cyanobacteria were examined for the presence of extrachromosomal DNA by using agarose gel electrophoresis and ethidium bromide staining. Seven of the 13 species contained extrachromosomal covalently closed circular DNA, and all but 1 species contained multiple elements. There was no correlation between the presence of extrachomosomal DNA and either the range of metabolic activities found in the cyanobacteria or the differentiated cell types or structures elaborated by the morphologically complex filamentous cyanobacteria.

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Selected References

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  1. Aaij C., Borst P. The gel electrophoresis of DNA. Biochim Biophys Acta. 1972 May 10;269(2):192–200. doi: 10.1016/0005-2787(72)90426-1. [DOI] [PubMed] [Google Scholar]
  2. Allen M. B., Arnon D. I. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. doi: 10.1104/pp.30.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Espejo R. T., Lebowitz J. A simple electrophoretic method for the determination of superhelix density of closed circular DNAs and for observation of their superhelix density heterogeneity. Anal Biochem. 1976 May 7;72:95–103. doi: 10.1016/0003-2697(76)90510-8. [DOI] [PubMed] [Google Scholar]
  5. Keller W. Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4876–4880. doi: 10.1073/pnas.72.12.4876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  7. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Roberts T. M., Koths K. E. The blue-green alga agmenellum quadruplicatum contains covalently closed DNA circles. Cell. 1976 Dec;9(4 Pt 1):551–557. doi: 10.1016/0092-8674(76)90037-4. [DOI] [PubMed] [Google Scholar]
  9. Simon R. D. Halobacterium strain 5 contains a plasmid which is correlated with the presence of gas vacuoles. Nature. 1978 May 25;273(5660):314–317. doi: 10.1038/273314a0. [DOI] [PubMed] [Google Scholar]
  10. Simon R. D. Sporulation in the filamentous cyanobacterium Anabaena cylindrica. The course of spore formation. Arch Microbiol. 1977 Jan 11;111(3):283–288. doi: 10.1007/BF00549367. [DOI] [PubMed] [Google Scholar]
  11. Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971 Jun;35(2):171–205. doi: 10.1128/br.35.2.171-205.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Studier F. W. Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli. J Bacteriol. 1975 Oct;124(1):307–316. doi: 10.1128/jb.124.1.307-316.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Witholt B., Boekhout M., Brock M., Kingma J., Heerikhuizen H. V., Leij L. D. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli. Anal Biochem. 1976 Jul;74(1):160–170. doi: 10.1016/0003-2697(76)90320-1. [DOI] [PubMed] [Google Scholar]
  14. Wolk C. P., Wojciuch E. Simple methods for plating single vegetative cells of, and for replica-plating, filamentous blue-green algae. Arch Mikrobiol. 1973 May 24;91(2):91–95. doi: 10.1007/BF00424753. [DOI] [PubMed] [Google Scholar]

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