Abstract
Previous studies have indicated that HLA-DR homozygous cell lines express two Ia alpha and Ia beta chains that combine to form at least two Ia molecules. This report demonstrates by two-dimensional gel electrophoresis the existence of a third structurally distinct human Ia beta chain on DR2 and DR5 cell lines. This suggests that at least five separate genes control the expression of Ia molecules on HLA-DR homozygous cell lines.
Full Text
The Full Text of this article is available as a PDF (414.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Corte G., Calabi F., Damiani G., Bargellesi A., Tosi R., Sorrentino R. Human Ia molecules carrying DC1 determinants differ in both alpha- and beta-subunits from Ia molecules carrying DR determinants. Nature. 1981 Jul 23;292(5821):357–360. doi: 10.1038/292357a0. [DOI] [PubMed] [Google Scholar]
- Delovitch T. L., Barber B. H. Evidence for two homologous, but nonidentical, Ia molecules determined by the I-EC subregion. J Exp Med. 1979 Jul 1;150(1):100–107. doi: 10.1084/jem.150.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goyert S. M., Silver J. Isolation of I-A subregion-like molecules from subhuman primates and man. Nature. 1981 Nov 19;294(5838):266–268. doi: 10.1038/294266a0. [DOI] [PubMed] [Google Scholar]
- Karr R. W., Kannapell C. C., Stein J. A., Gebel H. M., Mann D. L., Duquesnoy R. J., Fuller T. C., Rodey G. E., Schwartz B. D. Molecular relationships of the human B cell alloantigens, MT2, MB3, MT4, and DR5. J Immunol. 1982 Apr;128(4):1809–1818. [PubMed] [Google Scholar]
- Kratzin H., Yang C. Y., Götz H., Pauly E., Kölbel S., Egert G., Thinnes F. P., Wernet P., Altevogt P., Hilschmann N. Primärstruktur menschlicher Histokompatibilitätsantigene der Klasse II. 1. Mitteilung: Aminosäuresequenz der N-terminalen 198 Reste der beta-Kette des HLA-Dw2,2;DR2,2-Alloantigens. Hoppe Seylers Z Physiol Chem. 1981 Dec;362(12):1665–1669. [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Ozato K., Sachs D. H. Detection of at least two distinct mouse I-E antigen molecules by the use of a monoclonal antibody. J Immunol. 1982 Feb;128(2):807–810. [PubMed] [Google Scholar]
- Shackelford D. A., Mann D. L., van Rood J. J., Ferrara G. B., Strominger J. L. Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigen. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4566–4570. doi: 10.1073/pnas.78.7.4566. [DOI] [PMC free article] [PubMed] [Google Scholar]