Abstract
The characterization and distribution of cells containing the serum thymic factor (FTS) in the thymus of young mice was studied by immunofluorescence using monoclonal anti-FTS antibodies. FTS+ cells were distributed throughout the thymic parenchyma but were more frequent in the medullary region than in the cortex. FTS-containing cells presented a stellate or globular aspect, and some of them exhibited fluorescent cytoplasmic granules. The epithelial nature of FTS+ cells was confirmed by double-labeling experiments using an anti- keratin antiserum (as an epithelial cell marker). Nevertheless, only a minority of keratin-positive epithelial reticular cells contained FTS. All controls, including the incubation of sections from nonthymic tissues with the anti-FTS antibodies, were negative. Taken together, these results confirm the exclusive localization of FTS-containing cells within the mouse thymus.
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Selected References
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- Dardenne M., Pleau J. M., Lefrancier P., Bach J. F. Role du zinc et d'autres métaux dans l'activité biologique du FTS (thymuline). C R Seances Acad Sci III. 1981 Mar 30;292(13):793–796. [PubMed] [Google Scholar]
- Dardenne M., Pléau J. M., Blouquit J. Y., Bach J. F. Characterization of facteur thymique sérique (FTS) in the thymus. II. Direct demonstration of the presence of FTS in thymosin fraction V. Clin Exp Immunol. 1980 Dec;42(3):477–482. [PMC free article] [PubMed] [Google Scholar]
- Hirokawa K., McClure J. E., Goldstein A. L. Age-related changes in localization of thymosin in the human thymus. Thymus. 1982 Jan;4(1):19–29. [PubMed] [Google Scholar]
- Jambon B., Montagne P., Bene M. C., Brayer M. P., Faure G., Duheille J. Immunohistologic localization of "facteur thymique serique" (FTS) in human thymic epithelium. J Immunol. 1981 Nov;127(5):2055–2059. [PubMed] [Google Scholar]
- Kato K., Ikeyama S., Takaoki M., Shino A., Takeuchi M., Kakinuma A. Epithelial cell components immunoreact with anti-serum thymic factor (FTS) antibodies: possible association with intermediate-sized filaments. Cell. 1981 Jun;24(3):885–895. doi: 10.1016/0092-8674(81)90114-8. [DOI] [PubMed] [Google Scholar]
- Monier J. C., Dardenne M., Pléau J. M., Schmitt D., Deschaux P., Bach J. F. Characterization of facteur thymique sérique (FTS) in the thymus. I. Fixation of anti-FTS antibodies on thymic reticulo-epithelial cells. Clin Exp Immunol. 1980 Dec;42(3):470–476. [PMC free article] [PubMed] [Google Scholar]
- Papiernik M., Laroche L., Bach J. F. Thymocyte subpopulations in young and adult mice. I. Separation by density gradient and steroid treatment. Eur J Immunol. 1977 Nov;7(11):796–799. doi: 10.1002/eji.1830071110. [DOI] [PubMed] [Google Scholar]
- Papiernik M., Nabarra B. Thymic reticulum in mice. I. Cellular ultrastructure in vitro and functional role. Thymus. 1981 Dec;3(6):345–358. [PubMed] [Google Scholar]
- Pléau J. M., Fuentes V., Morgat J. L., Bach J. F. Specific receptors for the serum thymic factor (FTS) in lymphoblastoid cultured cell lines. Proc Natl Acad Sci U S A. 1980 May;77(5):2861–2865. doi: 10.1073/pnas.77.5.2861. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmitt D., Monier J. C., Dardenne M., Pleau J. M., Deschaux P., Bach J. F. Cytoplasmic localization of FTS (facteur thymique sérique) in thymic epithelial cells. An immunoelectronmicroscopical study. Thymus. 1980 Dec;2(3):177–186. [PubMed] [Google Scholar]
- Viac J., Staquet M. J., Thivolet J., Goujon C. Experimental production of antibodies against stratum corneum keratin polypeptides. Arch Dermatol Res. 1980;267(2):179–188. doi: 10.1007/BF00569104. [DOI] [PubMed] [Google Scholar]