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. 1982 Aug 1;156(2):443–453. doi: 10.1084/jem.156.2.443

Cell-to-cell interaction controlled by immunoglobulin genes. Role of Thy-1-, Lyt-1+, Ig+ (B') cell in allotype-restricted antibody production

PMCID: PMC2186767  PMID: 6124578

Abstract

A novel lymphocyte subpopulation, designated "B' cell" because of its characteristic dual expression of Ig and Lyt-1 antigen, is described in relation to its ability to augment the in vitro secondary antibody response. The cells are found in the spleens of normal unprimed mice as well as those of athymic nude mice and represent a small of normal unprimed mice as well as those of athymic nude mice and represent a small number (2-3%) of immunoglobulin-positive cells. No other distinguishing surface markers of conventional T and B cells, such as Thy-1, Lyt-2, Ia, and ThB antigens, are detected on the B' cell. In the in vitro anti-hapten secondary antibody response, the addition of a small number of B' cells from unprimed mice to the mixture of T and B cells greatly augmented the anti-hapten antibody formation when the number of carrier-specific helper T cells was limited. This augmentation was observed only when B and B' cells shared the same set of IgVH genes. The identity of the immunoglobulin gene between T cells and B or B' cells was not necessary for optimum antibody production. The results indicate that the presence of B' cells is necessary for the induction of an optimum antibody response when helper T cells are limited. It is suggested that B' cells deliver an additional signal to the B cell network to magnify the antibody response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adorini L., Harvey M., Sercarz E. E. The fine specificity of regulatory T cells. IV. Idiotypic complementarity and antigen-bridging interactions in the anti-lysozyme response. Eur J Immunol. 1979 Nov;9(11):906–909. doi: 10.1002/eji.1830091113. [DOI] [PubMed] [Google Scholar]
  2. Bottomly K., Mosier D. E. Mice whose B cells cannot produce the T15 idiotype also lack an antigen-specific helper T cell required for T15 expression. J Exp Med. 1979 Dec 1;150(6):1399–1409. doi: 10.1084/jem.150.6.1399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cantor H., Gershon R. K. Immunological circuits: cellular composition. Fed Proc. 1979 Jun;38(7):2058–2064. [PubMed] [Google Scholar]
  5. Eichmann K., Falk I., Melchers I., Simon M. M. Quantitative studies on T cell diversity. I. Determination of the precursor frequencies for two types of streptococcus A-specific helper cells in nonimmune, polyclonally activated splenic T cells. J Exp Med. 1980 Sep 1;152(3):477–492. doi: 10.1084/jem.152.3.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eichmann K., Falk I., Rajewsky K. Recognition of idiotypes in lymphocyte interactions. II. Antigen-independent cooperation between T and B lymphocytes that possess similar and complementary idiotypes. Eur J Immunol. 1978 Dec;8(12):853–857. doi: 10.1002/eji.1830081206. [DOI] [PubMed] [Google Scholar]
  7. Herzenberg L. A., Black S. J., Herzenberg L. A. Regulatory circuits and antibody responses. Eur J Immunol. 1980 Jan;10(1):1–11. doi: 10.1002/eji.1830100102. [DOI] [PubMed] [Google Scholar]
  8. Herzenberg L. A., Okumura K., Cantor H., Sato V. L., Shen F. W., Boyse E. A., Herzenberg L. A. T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells. J Exp Med. 1976 Aug 1;144(2):330–344. doi: 10.1084/jem.144.2.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Herzenberg L. A., Okumura K., Metzler C. M. Regulation of immunoglobulin and antibody production by allotype suppressor T cells in mice. Transplant Rev. 1975;27:57–83. doi: 10.1111/j.1600-065x.1975.tb00184.x. [DOI] [PubMed] [Google Scholar]
  10. Hetzelberger D., Eichmann K. Recognition of idiotypes in lymphocyte interactions. I. Idiotypic selectivity in the cooperation between T and B lymphocytes. Eur J Immunol. 1978 Dec;8(12):846–852. doi: 10.1002/eji.1830081205. [DOI] [PubMed] [Google Scholar]
  11. Janeway C. A., Jr, Bert D. L., Shen F. W. Cell cooperation during in vivo anti-hapten antibody responses. V. Two synergistic Ly-1+23- helper T cells with distinctive specificities. Eur J Immunol. 1980 Apr;10(4):231–236. doi: 10.1002/eji.1830100402. [DOI] [PubMed] [Google Scholar]
  12. Janeway C. A., Jr Cellular cooperation during in vivo anti-hapten antibody responses. I. The effect of cell number on the response. J Immunol. 1975 Apr;114(4):1394–1401. [PubMed] [Google Scholar]
  13. Janeway C. A., Jr Cellular cooperation during in vivo anti-hapten antibody responses. II. The effect of in vivo and in vitro x-irradiation on T and B cells. J Immunol. 1975 Apr;114(4):1402–1407. [PubMed] [Google Scholar]
  14. Janeway C. A., Jr Cellular cooperation during in vivo anti-hapten antibody responses. III. The helper cell activity of activated thymocytes, of spleen cells treated with anti-theta serum, and of spleen cells from anti-thymocyte serum-treated or adult thymectomized donors. J Immunol. 1975 Apr;114(4):1408–1414. [PubMed] [Google Scholar]
  15. Janeway C. A., Jr, Murgita R. A., Weinbaum F. I., Asofsky R., Wigzell H. Evidence for an immunoglobulin-dependent antigen-specific helper T cell. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4582–4586. doi: 10.1073/pnas.74.10.4582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. L'Age-Stehr J. Priming of T helper cells by antigen-activated B cells. B cell-primed Lyt-1+ helper cells are restricted to cooperate with B cells expressing the IgvH phenotype of the priming B cells. J Exp Med. 1981 May 1;153(5):1236–1245. doi: 10.1084/jem.153.5.1236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280–295. doi: 10.1084/jem.152.2.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marrack P. C., Kappler J. W. Antigen-specific and nonspecific mediators of T cell/B cell cooperation. I. Evidence for their production by different T cells. J Immunol. 1975 Mar;114(3):1116–1125. [PubMed] [Google Scholar]
  19. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  20. Nutt N., Haber J., Wortis H. H. Influence of Igh-linked gene products on the generation of T helper cells in the response to sheep erythrocytes. J Exp Med. 1981 May 1;153(5):1225–1235. doi: 10.1084/jem.153.5.1225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Okumura K., Takemori T., Tokuhisa T., Tada T. Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations. J Exp Med. 1977 Nov 1;146(5):1234–1245. doi: 10.1084/jem.146.5.1234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Riblet R., Blomberg B., Weigert M., Lieberman R., Taylor B. A., Potter M. Genetics of mouse antibodies. I. Linkage of the dextran response locus, VH-DEX, to allotype. Eur J Immunol. 1975 Nov;5(11):775–777. doi: 10.1002/eji.1830051109. [DOI] [PubMed] [Google Scholar]
  23. Tada T., Takemori T., Okumura K., Nonaka M., Tokuhisa T. Two distinct types of helper T cells involved in the secondary antibody response: independent and synergistic effects of Ia- and Ia+ helper T cells. J Exp Med. 1978 Feb 1;147(2):446–458. doi: 10.1084/jem.147.2.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Woodland R., Cantor H. Idiotype-specific T helper cells are required to induce idiotype-positive B memory cells to secrete antibody. Eur J Immunol. 1978 Aug;8(8):600–606. doi: 10.1002/eji.1830080812. [DOI] [PubMed] [Google Scholar]
  25. Yutoku M., Grossberg A. L., Stout R., Herzenberg L. A., Pressman D. Further studies on Th-B, a cell surface antigenic determinant present on mouse B cells, plasma cells and immature thymocytes. Cell Immunol. 1976 Apr;23(1):140–157. doi: 10.1016/0008-8749(76)90178-7. [DOI] [PubMed] [Google Scholar]

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