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. 1978 Oct;136(1):433–436. doi: 10.1128/jb.136.1.433-436.1978

Levels of cyclic GMP in dormant, germinated, and outgrowing spores and growing and sporulating cells of Bacillus megaterium.

B Setlow, P Setlow
PMCID: PMC218677  PMID: 213418

Abstract

The level of cyclic GMP was less than one molecule per organism in dormant, germinated, and outgrowing spores of Bacillus megaterium. A significant level (approximately 8 pmol/g, dry weight) of cyclic GMP was found in early to mid-log phase cells, but the level fell to below 0.2 pmol/g, dry weight, in late-log phase and only rose slightly to approximately 0.9 pmol/g, dry weight, in stationary phare. No significant amount of cyclic GMP was detected in the growth medium at any time.

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Selected References

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  1. Bernlohr R. W., Haddox M. K., Goldberg N. D. Cyclic guanosine 3':5'-monophosphate in Escherichia coli and Bacillus lichenformis. J Biol Chem. 1974 Jul 10;249(13):4329–4331. [PubMed] [Google Scholar]
  2. Cailla H. L., Vannier C. J., Delaage M. A. Guanosine 3', 5'-cyclicmonophosphate assay at 10(-15)-mole level. Anal Biochem. 1976 Jan;70(1):195–202. doi: 10.1016/s0378-5173(83)90100-x. [DOI] [PubMed] [Google Scholar]
  3. Clark V. L., Bernlohr R. W. Guanyl cyclase of Bacillus licheniformis. Biochem Biophys Res Commun. 1972 Feb 25;46(4):1570–1575. doi: 10.1016/0006-291x(72)90787-5. [DOI] [PubMed] [Google Scholar]
  4. Gonzalez J. E., Peterkofsky A. Diverse directional changes of cGMP relative to cAMP in E. coli. Biochem Biophys Res Commun. 1975 Nov 3;67(1):190–197. doi: 10.1016/0006-291x(75)90301-0. [DOI] [PubMed] [Google Scholar]
  5. Hwang T. C., Clark V. L., Bernlohr R. W. Guanosine 3', 5'-cyclic monophosphate phosphodiesterase activity of Bacillus licheniformis. Biochem Biophys Res Commun. 1974 Jun 4;58(3):707–713. doi: 10.1016/s0006-291x(74)80475-4. [DOI] [PubMed] [Google Scholar]
  6. Kaziro Y. Accumulation of cyclic guanosine 3':5'-monophosphate in the culture medium of growing cells of Escherichia coli. Biochem Biophys Res Commun. 1976 Jan 26;68(2):430–435. doi: 10.1016/0006-291x(76)91163-3. [DOI] [PubMed] [Google Scholar]
  7. Setlow P. Inability of detect cyclic AMP in vegetative or sporulating cells or dormant spores of Bacillus megaterium. Biochem Biophys Res Commun. 1973 May 15;52(2):365–372. doi: 10.1016/0006-291x(73)90720-1. [DOI] [PubMed] [Google Scholar]
  8. Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. XVII. Sulfhydryl and disulfide levels in dormancy and germination. J Bacteriol. 1969 Dec;100(3):1155–1160. doi: 10.1128/jb.100.3.1155-1160.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores. J Biol Chem. 1970 Jul 25;245(14):3637–3644. [PubMed] [Google Scholar]
  10. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Steiner A. L., Parker C. W., Kipnis D. M. Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides. J Biol Chem. 1972 Feb 25;247(4):1106–1113. [PubMed] [Google Scholar]

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