Abstract
During the time of tissue repair that ensues subsequent to tissue injury, blood vessel wall fibronectin increases concomitantly with endothelial proliferation and angiogenesis. However, the source of this blood vessel fibronectin had not been delineated. In this report we have demonstrated that microvascular fibronectin is produced in situ by the proliferating vessels surrounding excisional wounds. This finding was established by extirpating 3 mm of skin from the center of a well- healed rat xenograph on the flanks of immunosuppressed mice, harvesting the injured skin sites at various stages during the healing process, and staining the specimens with reciprocal species-specific anti- fibronectin. The proliferating donor vessels that surrounded the wounded graft had increased fluorescence staining with FITC conjugated mouse anti-rat fibronectin and no staining with rat anti-mouse fibronectin. This finding was taken as direct evidence that the fibronectin was produced in situ by the rat vessels and not derived from circulating mouse plasma.
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Selected References
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- Alitalo K., Hovi T., Vaheri A. Fibronectin is produced by human macrophages. J Exp Med. 1980 Mar 1;151(3):602–613. doi: 10.1084/jem.151.3.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baum B. J., McDonald J. A., Crystal R. G. Metabolic fate of the major cell surface protein of normal human fibroblasts. Biochem Biophys Res Commun. 1977 Nov 7;79(1):8–15. doi: 10.1016/0006-291x(77)90053-5. [DOI] [PubMed] [Google Scholar]
- Birdwell C. R., Gospodarowicz D., Nicolson G. L. Identification, localization, and role of fibronectin in cultured bovine endothelial cells. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3273–3277. doi: 10.1073/pnas.75.7.3273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark R. A., Dvorak H. F., Colvin R. B. Fibronectin in delayed-type hypersensitivity skin reactions: associations with vessel permeability and endothelial cell activation. J Immunol. 1981 Feb;126(2):787–793. [PubMed] [Google Scholar]
- Critchley D. R., England M. A., Wakely J., Hynes R. O. Distribution of fibronectin in the ectoderm of gastrulating chick embryos. Nature. 1979 Aug 9;280(5722):498–500. doi: 10.1038/280498a0. [DOI] [PubMed] [Google Scholar]
- Engvall E., Ruoslahti E. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer. 1977 Jul 15;20(1):1–5. doi: 10.1002/ijc.2910200102. [DOI] [PubMed] [Google Scholar]
- Fujikawa L. S., Foster C. S., Harrist T. J., Lanigan J. M., Colvin R. B. Fibronectin in healing rabbit corneal wounds. Lab Invest. 1981 Aug;45(2):120–129. [PubMed] [Google Scholar]
- Grinnell F., Billingham R. E., Burgess L. Distribution of fibronectin during wound healing in vivo. J Invest Dermatol. 1981 Mar;76(3):181–189. doi: 10.1111/1523-1747.ep12525694. [DOI] [PubMed] [Google Scholar]
- Hayman E. G., Ruoslahti E. Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix. J Cell Biol. 1979 Oct;83(1):255–259. doi: 10.1083/jcb.83.1.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hynes R. O. Alteration of cell-surface proteins by viral transformation and by proteolysis. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170–3174. doi: 10.1073/pnas.70.11.3170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaffe E. A., Mosher D. F. Synthesis of fibronectin by cultured human endothelial cells. J Exp Med. 1978 Jun 1;147(6):1779–1791. doi: 10.1084/jem.147.6.1779. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jooste S. V., Colvin R. B., Soper W. D., Winn H. J. The vascular bed as the primary target in the destruction of skin grafts by antiserum. I. Resistance of freshly placed xenografts of skin to antiserum. J Exp Med. 1981 Nov 1;154(5):1319–1331. doi: 10.1084/jem.154.5.1319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jooste S. V., Colvin R. B., Winn H. J. The vascular bed as the primary target in the destruction of skin grafts by antiserum. II. Loss of sensitivity to antiserum in long-term xenografts of skin. J Exp Med. 1981 Nov 1;154(5):1332–1341. doi: 10.1084/jem.154.5.1332. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
- Klebe R. J. Isolation of a collagen-dependent cell attachment factor. Nature. 1974 Jul 19;250(463):248–251. doi: 10.1038/250248a0. [DOI] [PubMed] [Google Scholar]
- Kurkinen M., Vaheri A., Roberts P. J., Stenman S. Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest. 1980 Jul;43(1):47–51. [PubMed] [Google Scholar]
- Linder E., Vaheri A., Ruoslahti E., Wartiovaara J. Distribution of fibroblast surface antigen in the developing chick embryo. J Exp Med. 1975 Jul 1;142(1):41–49. doi: 10.1084/jem.142.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macarak E. J., Kirby E., Kirk T., Kefalides N. A. Synthesis of cold-insoluble globulin by cultured calf endothelial cells. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2621–2625. doi: 10.1073/pnas.75.6.2621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pearlstein E. Plasma membrane glycoprotein which mediates adhesion of fibroblasts to collagen. Nature. 1976 Aug 5;262(5568):497–500. doi: 10.1038/262497a0. [DOI] [PubMed] [Google Scholar]
- Ruoslahti E., Vaheri A. Novel human serum protein from fibroblast plasma membrane. Nature. 1974 Apr 26;248(5451):789–791. doi: 10.1038/248789a0. [DOI] [PubMed] [Google Scholar]
- Yamada K. M., Weston J. A. Isolation of a major cell surface glycoprotein from fibroblasts. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3492–3496. doi: 10.1073/pnas.71.9.3492. [DOI] [PMC free article] [PubMed] [Google Scholar]