Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1982 Sep 1;156(3):719–730. doi: 10.1084/jem.156.3.719

Immunoglobulin idiotopes expressed by T cells. I. Expression of distinct idiotopes detected by monoclonal antibodies on antigen- specific suppressor T cells

PMCID: PMC2186786  PMID: 6213731

Abstract

The idiotopic repertoire expressed by antigen-specific suppressor T cells (Ts) generated by Streptococcus pneumoniae strain R36a (Pn) in BALB/c strain mice was investigated using a panel of five monoclonal anti-idiotopic antibodies against TEPC-15/HOPC-8 myeloma proteins. Previous studies suggested that the anti-idiotopic antibodies recognize distinct idiotopic determinants within the T15 idiotype, and that Pn- reactive B cells express all of those idiotopes as shown by a specific inhibitory effect of the anti-idiotopic antibodies on induction of anti- Pn response in vitro as well as on the mature antibody plaque-forming cells. In this study we asked the question of whether anti-idiotopic (Id) can block the inductive and/or effector phases of generation of Ts which act on the Pn-reactive B cells. The presence of anti-Id during the activation of T cells with Pn did not prevent the generation of Ts. However, suppression mediated by Ts on responder lymphocytes (cultures of spleen cells or B cels) was inhibited (reversed) by four out of five anti-Id. Some of the antibodies recognize hapten (phosphorylcholine)- inhibitable Id in the paratope of Ig whereas others are directed against nonparatopic Id. These data indicate that the antigen receptor on Ts includes VH sequences both within and without the immunoglobulin in paratope, and that the Id repertoir of Ts overlaps with that of B cells.

Full Text

The Full Text of this article is available as a PDF (985.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Augustin A., Cosenza H. Expression of new idiotypes following neonatal idiotypic suppression of a dominant clone. Eur J Immunol. 1976 Jul;6(7):497–501. doi: 10.1002/eji.1830060710. [DOI] [PubMed] [Google Scholar]
  2. Benca R., Quintáns J., Kearney J. F., Flood P. M., Schreiber H. Studies on phosphorylcholine-specific T cell idiotypes and idiotype-specific immunity. Mol Immunol. 1980 Jul;17(7):823–831. doi: 10.1016/0161-5890(80)90031-0. [DOI] [PubMed] [Google Scholar]
  3. Binz H., Wigzell H. Antigen-binding, idiotypic T-lymphocyte receptors. Contemp Top Immunobiol. 1977;7:113–177. doi: 10.1007/978-1-4684-3054-7_4. [DOI] [PubMed] [Google Scholar]
  4. Caulfield M. J., Cerny J. Cell interactions in leukemia-associated immunosuppression: suppression of thymus-independent antibody responses by leukemia spleen cells (Moloney) in vitro is mediated by normal T cells. J Immunol. 1980 Jan;124(1):255–260. [PubMed] [Google Scholar]
  5. Cerny J., Caulfield M. J., Siat M. Regulation of the T 15 idiotype-positive, T-independent immune response to Streptococcus pneumoniae R 36 a (Pn). I. Specific suppressor T cells induced by Pn in vitro are antigen-specific and idiotype-positive. Eur J Immunol. 1980 Sep;10(9):661–664. doi: 10.1002/eji.1830100902. [DOI] [PubMed] [Google Scholar]
  6. Cerny J., Wallich R., Hammerling G. J. Analysis of T15 idiotopes by monoclonal antibodies: variability of idiotopic expression on phosphorylcholine-specific lymphocytes from individual inbred mice. J Immunol. 1982 Apr;128(4):1885–1891. [PubMed] [Google Scholar]
  7. Chang S. P., Rittenberg M. B. Immunologic memory to phosphorylcholine in vitro. I. Asymmetric expression of clonal dominance. J Immunol. 1981 Mar;126(3):975–980. [PubMed] [Google Scholar]
  8. Dzierzak E. A., Rosenstein R. W., Janeway C. A., Jr Expression of an idiotype (Id-460) during in vivo anti-dinitrophenyl antibody responses. II. Transient idiotypic dominance. J Exp Med. 1981 Nov 1;154(5):1432–1441. doi: 10.1084/jem.154.5.1432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eardley D. D., Gershon R. K. Induction of specific suppressor T cells in vitro. J Immunol. 1976 Jul;117(1):313–318. [PubMed] [Google Scholar]
  10. Eichmann K. Expression and function of idiotypes of lymphocytes. Adv Immunol. 1978;26:195–254. doi: 10.1016/s0065-2776(08)60231-x. [DOI] [PubMed] [Google Scholar]
  11. Estess P., Lamoyi E., Nisonoff A., Capra J. D. Structural studies on induced antibodies with defined idiotypic specificities. IX. Framework differences in the heavy- and light-chain-variable regions of monoclonal anti-p-azophenylarsonate antibodies from A/J mice differing with respect to a cross-reactive idiotype. J Exp Med. 1980 Apr 1;151(4):863–875. doi: 10.1084/jem.151.4.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grinwich K. D., Alexander T. S., Cerny J. Evidence for a role for calcium in immunosuppression by tumor cells in vitro. Cell Immunol. 1978 May;37(2):285–297. doi: 10.1016/0008-8749(78)90197-1. [DOI] [PubMed] [Google Scholar]
  13. Harvey M. A., Adorini L., Miller A., Sercarz E. E. Lysozyme-induced T-suppressor cells and antibodies have a predominant idiotype. Nature. 1979 Oct 18;281(5732):594–596. doi: 10.1038/281594a0. [DOI] [PubMed] [Google Scholar]
  14. Kearney J. F., Barletta R., Quan Z. S., Quintáns J. Monoclonal vs. heterogeneous anti-H-8 antibodies in the analysis of the anti-phosphorylcholine response in BALB/c mice. Eur J Immunol. 1981 Nov;11(11):877–883. doi: 10.1002/eji.1830111106. [DOI] [PubMed] [Google Scholar]
  15. Kelsoe G., Isaak D., Cerny J. Thymic requirement for cyclical idiotypic and reciprocal anti-idiotypic immune responses to a T-independent antigen. J Exp Med. 1980 Feb 1;151(2):289–300. doi: 10.1084/jem.151.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kelsoe G., Reth M., Rajewsky K. Control idiotope expression by monoclonal anti-idiotope antibodies. Immunol Rev. 1980;52:75–88. doi: 10.1111/j.1600-065x.1980.tb00331.x. [DOI] [PubMed] [Google Scholar]
  17. Krawinkel U., Cramer M., Melchers I., Imanishi-Kari T., Rajewsky K. Isolated hapten-binding receptors of sensitized lymphocytes. III. Evidence for idiotypic restriction of T-cell receptors. J Exp Med. 1978 May 1;147(5):1341–1347. doi: 10.1084/jem.147.5.1341. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Köhler G., Howe S. C., Milstein C. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur J Immunol. 1976 Apr;6(4):292–295. doi: 10.1002/eji.1830060411. [DOI] [PubMed] [Google Scholar]
  19. Köhler H., Kaplan D. R., Strayer D. S. Clonal depletion in neonatal tolerance. Science. 1974 Nov 15;186(4164):643–644. doi: 10.1126/science.186.4164.643. [DOI] [PubMed] [Google Scholar]
  20. Lamoyi E., Estess P., Capra J. D., Nisonoff A. Heterogeneity of an intrastrain cross-reactive idiotype associated with anti-p-azophenylarsonate antibodies of A/J mice. J Immunol. 1980 Jun;124(6):2834–2840. [PubMed] [Google Scholar]
  21. Lewis G. K., Goodman J. W. Purification of functional, determinant-specific, idiotype-bearing murine T cells. J Exp Med. 1978 Oct 1;148(4):915–924. doi: 10.1084/jem.148.4.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Marshak-Rothstein A., Fink P., Gridley T., Raulet D. H., Bevan M. J., Gefter M. L. Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol. 1979 Jun;122(6):2491–2497. [PubMed] [Google Scholar]
  23. Marshak-Rothstein A., Siekevitz M., Margolies M. N., Mudgett-Hunter M., Gefter M. L. Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1120–1124. doi: 10.1073/pnas.77.2.1120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pillemer E., Weissman I. L. A monoclonal antibody that detects a V kappa-TEPC15 idiotypic determinant cross-reactive with a Thy-1 determinant. J Exp Med. 1981 May 1;153(5):1068–1079. doi: 10.1084/jem.153.5.1068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Reth M., Imanishi-Kari T., Rajewsky K. Analysis of the repertoire of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur J Immunol. 1979 Dec;9(12):1004–1013. doi: 10.1002/eji.1830091216. [DOI] [PubMed] [Google Scholar]
  26. Reth M., Kelsoe G., Rajewsky K. Idiotypic regulation by isologous monoclonal anti-idiotope antibodies. Nature. 1981 Mar 19;290(5803):257–259. doi: 10.1038/290257a0. [DOI] [PubMed] [Google Scholar]
  27. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  28. Rubin A. S., Coons A. H. Specific heterologous enhancement of immune responses. 3. Partial characterization of supernatant material with enhancing activity. J Immunol. 1972 Jun;108(6):1597–1604. [PubMed] [Google Scholar]
  29. Schreiber A. B., Couraud P. O., Andre C., Vray B., Strosberg A. D. Anti-alprenolol anti-idiotypic antibodies bind to beta-adrenergic receptors and modulate catecholamine-sensitive adenylate cyclase. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7385–7389. doi: 10.1073/pnas.77.12.7385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sege K., Peterson P. A. Use of anti-idiotypic antibodies as cell-surface receptor probes. Proc Natl Acad Sci U S A. 1978 May;75(5):2443–2447. doi: 10.1073/pnas.75.5.2443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Weinberger J. Z., Germain R. N., Ju S. T., Greene M. I., Benacerraf B., Dorf M. E. Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. II. Demonstration of idiotypic determinants on suppressor T cells. J Exp Med. 1979 Oct 1;150(4):761–776. doi: 10.1084/jem.150.4.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES