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. 1982 Dec 1;156(6):1577–1586. doi: 10.1084/jem.156.6.1577

Study on cellular events in post-thymectomy autoimmune oophoritis in mice. II. Requirement of Lyt-1 cells in normal female mice for the prevention of oophoritis

PMCID: PMC2186864  PMID: 6983558

Abstract

Autoimmune oophoritis that develops in A/J mice after neonatally thymectomy (NTx) was prevented by a single intraperitoneal injection of spleen cells or thymocytes from normal adult female mice. Prevention of oophoritis was achieved when spleen cells were given within 2 wk after Tx. When spleen cells were obtained from neonatally oophorectomized mice, four times more cells were required for the prevention of oophoritis, but those from the mice oophorectomized on day 7 after birth had equivalent capacity to prevent oophoritis to those from normal female mice. The spleen cells from normal A/J mice that prevented the development of oophoritis in NTx A/J mice were Thy-1+, Lyt-1+,23-, Ia-, Qa-1-, sensitive to in vitro irradiation with 400 rad, resistant to administration of cyclophosphamide or anti-thymocyte serum, and were not eliminated by adult thymectomy. Thymocytes with oophoritis-preventing capacity were also found to be Lyt-1+,23- and TL- 1,2,3-. These results seem to correlate well with the finding that the Lyt-1 subpopulation is substantially decreased in NTx mice. The results suggest that, in this post-thymectomy autoimmune oophoritis, NTx abrogates the Lyt-1 T cell subpopulation that serves as suppressive or regulatory cells over developing self-reactive cells directed toward ovarian antigens, and eventually may cause autoimmune oophoritis.

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Selected References

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  1. Askenase P. W., Hayden B. J., Gershon R. K. Augmentation of delayed-type hypersensitivity by doses of cyclophosphamide which do not affect antibody responses. J Exp Med. 1975 Mar 1;141(3):697–702. doi: 10.1084/jem.141.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOYSE E. A., OLD L. J., CHOUROULINKOV I. CYTOTOXIC TEST FOR DEMONSTRATION OF MOUSE ANTIBODY. Methods Med Res. 1964;10:39–47. [PubMed] [Google Scholar]
  3. Cantor H., McVay-Boudreau L., Hugenberger J., Naidorf K., Shen F. W., Gershon R. K. Immunoregulatory circuits among T-cell sets. II. Physiologic role of feedback inhibition in vivo: absence in NZB mice. J Exp Med. 1978 Apr 1;147(4):1116–1125. doi: 10.1084/jem.147.4.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eardley D. D., Hugenberger J., McVay-Boudreau L., Shen F. W., Gershon R. K., Cantor H. Immunoregulatory circuits among T-cell sets. I. T-helper cells induce other T-cell sets to exert feedback inhibition. J Exp Med. 1978 Apr 1;147(4):1106–1115. doi: 10.1084/jem.147.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Herzenberg L. A., Okumura K., Cantor H., Sato V. L., Shen F. W., Boyse E. A., Herzenberg L. A. T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells. J Exp Med. 1976 Aug 1;144(2):330–344. doi: 10.1084/jem.144.2.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  7. Kojima A., Taguchi O., Nishizuka Y. Experimental production of possible autoimmune castritis followed by macrocytic anemia in athymic nude mice. Lab Invest. 1980 Apr;42(4):387–395. [PubMed] [Google Scholar]
  8. Kojima A., Tanaka-Kojima Y., Sakakura T., Nishizuka Y. Prevention of postthymectomy autoimmune thyroiditis in mice. Lab Invest. 1976 Jun;34(6):601–605. [PubMed] [Google Scholar]
  9. Kojima A., Tanaka-Kojima Y., Sakakura T., Nishizuka Y. Spontaneous development of autoimmune thyroiditis in neonatally thymectomized mice. Lab Invest. 1976 Jun;34(6):550–557. [PubMed] [Google Scholar]
  10. Liew F. Y., Russell S. M. Delayed-type hypersensitivity to influenza virus. Induction of antigen-specific suppressor T cells for delayed-type hypersensitivity to hemagglutinin during influenza virus infection in mice. J Exp Med. 1980 Apr 1;151(4):799–814. doi: 10.1084/jem.151.4.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mathieson B. J., Sharrow S. O., Campbell P. S., Asofsky R. An Lyt differentiated thymocyte subpopulation detected by flow microfluorometry. Nature. 1979 Feb 8;277(5696):478–480. doi: 10.1038/277478a0. [DOI] [PubMed] [Google Scholar]
  12. Nishizuka Y., Sakakura T. Thymus and reproduction: sex-linked dysgenesia of the gonad after neonatal thymectomy in mice. Science. 1969 Nov 7;166(3906):753–755. doi: 10.1126/science.166.3906.753. [DOI] [PubMed] [Google Scholar]
  13. Penhale W. J., Farmer A., Irvine W. J. Thyroiditis in T cell-depleted rats. Influence of strain, radiation dose, adjuvants and antilymphocyte serum. Clin Exp Immunol. 1975 Sep;21(3):362–375. [PMC free article] [PubMed] [Google Scholar]
  14. Penhale W. J., Irvine W. J., Inglis J. R., Farmer A. Thyroiditis in T cell-depleted rats: suppression of the autoallergic response by reconstitution with normal lymphoid cells. Clin Exp Immunol. 1976 Jul;25(1):6–16. [PMC free article] [PubMed] [Google Scholar]
  15. Potter T. A., Hogarth P. M., McKenzie I. F. Flow microfluorometric analysis of alloantigen expression during T cell development. Eur J Immunol. 1980 Dec;10(12):899–903. doi: 10.1002/eji.1830101202. [DOI] [PubMed] [Google Scholar]
  16. Sakakura T., Nishizuka Y. Thymic control mechanism in ovarian development: reconstitution of ovarian dysgenesis in thymectomized mice by replacement with thymic and other lymphoid tissues. Endocrinology. 1972 Feb;90(2):431–437. doi: 10.1210/endo-90-2-431. [DOI] [PubMed] [Google Scholar]
  17. Sy M. S., Dietz M. H., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induce second-order suppressor T cells which express anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1183–1195. doi: 10.1084/jem.151.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taguchi O., Nishizuka Y. Experimental autoimmune orchitis after neonatal thymectomy in the mouse. Clin Exp Immunol. 1981 Nov;46(2):425–434. [PMC free article] [PubMed] [Google Scholar]
  19. Taniguchi M., Saito T., Tada T. Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma. Nature. 1979 Apr 5;278(5704):555–558. doi: 10.1038/278555a0. [DOI] [PubMed] [Google Scholar]
  20. Weigle W. O. Analysis of autoimmunity through experimental models of thyroiditis and allergic encephalomyelitis. Adv Immunol. 1980;30:159–273. doi: 10.1016/s0065-2776(08)60196-0. [DOI] [PubMed] [Google Scholar]
  21. Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]

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