Abstract
Three distinct molecular subsets with different structures and alloantigenic determinants were identified in human Ia antigens from cells of an HLA-Dw7 homozygous cell line. The subsets carried DR7 specificity, BR4X7 supertypic specificity and MB2 supertypic specificity, respectively, and were immunospecifically separated by the use of operationally monospecific alloantisera. These specificities showed HLA-linked segregation in families and they were distributed in the population according to different but partially overlapping patterns. On peptide mapping analysis, the three subsets showed marked differences in the beta-chains. The alpha-chains of DR7 and BR4X7 subsets were very similar to each other, whereas the alpha-chains of MB2 subset were distinctive from those of DR7 and BR4X7. These data indicate the presence of a minimum of three HLA-linked loci; DR locus, a locus that encodes BR4X7, and a locus that encodes MB2, and substantiate the three-loci concept for the genetic control of human I antigens.
Full Text
The Full Text of this article is available as a PDF (1.5 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cook R. G., Siegelman M. H., Capra J. D., Uhr J. W., Vitetta E. S. Structural studies on the murine Ia alloantigens. IV. NH2-terminal sequence analysis of allelic products of the I-A and I-E/C subregions. J Immunol. 1979 Jun;122(6):2232–2237. [PubMed] [Google Scholar]
- Corte G., Calabi F., Damiani G., Bargellesi A., Tosi R., Sorrentino R. Human Ia molecules carrying DC1 determinants differ in both alpha- and beta-subunits from Ia molecules carrying DR determinants. Nature. 1981 Jul 23;292(5821):357–360. doi: 10.1038/292357a0. [DOI] [PubMed] [Google Scholar]
- Corte G., Damiani G., Calabi F., Fabbi M., Bargellesi A. Analysis of HLA-DR polymorphism by two-dimensional peptide mapping. Proc Natl Acad Sci U S A. 1981 Jan;78(1):534–538. doi: 10.1073/pnas.78.1.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cullen S. E., Shreffler D. C., Kindle C. S., David C. S. Identity of molecules bearing murine Ia alloantigenic specificities Ia.7 and Ia.22: evidence for a single type of I-E molecule in homozygotes. Immunogenetics. 1980;11(6):535–547. doi: 10.1007/BF01567823. [DOI] [PubMed] [Google Scholar]
- Duquesnoy R. J., Marrari M., Annen K. Identification of an HLA-DR-associated system of B-cell alloantigens. Transplant Proc. 1979 Dec;11(4):1757–1760. [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayman M. J., Crumpton M. J. Isolation of glycoproteins from pig lymphocyte plasma membrane using Lens culinaris phytohemagglutinin. Biochem Biophys Res Commun. 1972 May 26;47(4):923–930. doi: 10.1016/0006-291x(72)90581-5. [DOI] [PubMed] [Google Scholar]
- Kaufman J. F., Andersen R. L., Strominger J. L. HLA-DR antigens have polymorphic light chains and invariant heavy chains as assessed by lysine-containing tryptic peptide analysis. J Exp Med. 1980 Aug 1;152(2 Pt 2):37s–53s. [PubMed] [Google Scholar]
- Koyama K., Nakamuro K., Tanigaki N., Pressman D. Alloantigens of human lymphoid cell lines; 'human Ia-like antigens'. Alloantigenic activity and cell line, organ and tissue distribution as determined by radioimmunoassay. Immunology. 1977 Aug;33(2):217–230. [PMC free article] [PubMed] [Google Scholar]
- Markert M. L., Cresswell P. Polymorphism of human B-cell alloantigens: evidence for three loci within the HLA system. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6101–6104. doi: 10.1073/pnas.77.10.6101. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shackelford D. A., Mann D. L., van Rood J. J., Ferrara G. B., Strominger J. L. Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigen. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4566–4570. doi: 10.1073/pnas.78.7.4566. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanigaki N., Tosi R. Assessment of the specificity of human Ia alloantisera and alloantigens by the use of radioiodinated human Ia antigens. Tissue Antigens. 1982 Jul;20(1):1–21. doi: 10.1111/j.1399-0039.1982.tb00324.x. [DOI] [PubMed] [Google Scholar]
- Tanigaki N., Tosi R., Koyama K., Pressman D. Purification and separation of subsets of human Ia molecules by papain digestion. Immunology. 1980 Apr;39(4):615–622. [PMC free article] [PubMed] [Google Scholar]
- Tanigaki N., Tosi R., Pressman D., Ferrara G. B. Molecular identification of human Ia antigens coded for by a gene locus closely linked to HLA-DR locus. Immunogenetics. 1980;10(2):151–167. doi: 10.1007/BF01561564. [DOI] [PubMed] [Google Scholar]
- Tanigaki N., Tosi R. The genetic control of human Ia alloantigens: a three-loci model derived from the immunochemical analysis of 'supertypic' specificities. Immunol Rev. 1982;66:5–37. doi: 10.1111/j.1600-065x.1982.tb00432.x. [DOI] [PubMed] [Google Scholar]
- Tosi R., Tanigaki N., Centis D., Ferrara G. B., Pressman D. Immunological dissection of human Ia molecules. J Exp Med. 1978 Dec 1;148(6):1592–1611. doi: 10.1084/jem.148.6.1592. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tosi R., Tanigaki N., Centis D., Rossi P. L., Alfano G., Ferrara G. B., Pressman D. HLA-DR typing by radioimmunoassay. Transplantation. 1980 Apr;29(4):302–305. doi: 10.1097/00007890-198004000-00008. [DOI] [PubMed] [Google Scholar]