Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1983 Jan 1;157(1):15–23. doi: 10.1084/jem.157.1.15

Passive transfer of the idiotypically suppressed state by serum from suppressed mice and transfer of suppression from mothers to offspring

PMCID: PMC2186897  PMID: 6217277

Abstract

Mice that are suppressed with respect to an idiotype (CRIA) present in A/J anti-p-azophenylarsonate antibodies, hyperimmunized, and allowed to rest were previously found to possess high concentrations of suppressor T cells with anti-idiotypic receptors. We have now observed that the sera of such mice contain soluble factors that can selectively suppress the CRIA component of a humoral response when passively transferred to adult or neonatal recipients. When T cells from suppressed, hyperimmunized mice were transferred into female mice before mating, their offspring, upon immunization, produced anti-Ar antibodies that lacked CRIA. A state of idiotypic suppression was also produced in offspring when the mother was inoculated with serum from suppressed mice a few days before parturition. The results indicate that the suppressor factor is not an immunoglobulin.

Full Text

The Full Text of this article is available as a PDF (668.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernabé R. R., Coutinho A., Cazenave P. A., Forni L. Suppression of a "recurrent" idiotype results in profound alterations of the whole B-cell compartment. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6416–6420. doi: 10.1073/pnas.78.10.6416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bona C., Stein K. E., Lieberman R., Paul W. E. Direct and indirect suppression induced by anti-idiotype antibody in the inulin-bacterial levan antigenic system. Mol Immunol. 1979 Dec;16(12):1093–1101. doi: 10.1016/0161-5890(79)90043-9. [DOI] [PubMed] [Google Scholar]
  3. Cosenza H., Julius M. H., Augustin A. A. Idiotypes as variable region markers: analogies between receptors on phosphorylcholine-specific T and B lymphocytes. Immunol Rev. 1977;34:3–33. doi: 10.1111/j.1600-065x.1977.tb00366.x. [DOI] [PubMed] [Google Scholar]
  4. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  5. DRAY S. Effect of maternal isoantibodies on the quantitative expression of two allelic genes controlling gamma-globulin allotypic specificities. Nature. 1962 Aug 18;195:677–680. doi: 10.1038/195677a0. [DOI] [PubMed] [Google Scholar]
  6. Dohi Y., Nisonoff A. Suppression of idiotype and generation of suppressor T cells with idiotype-conjugated thymocytes. J Exp Med. 1979 Oct 1;150(4):909–918. doi: 10.1084/jem.150.4.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hart D. A., Wang A. L., Pawlak L. L., Nisonoff A. Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody. J Exp Med. 1972 Jun 1;135(6):1293–1300. doi: 10.1084/jem.135.6.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirai Y., Nisonoff A. Selective suppression of the major idiotypic component of an antihapten response by soluble T cell-derived factors with idiotypic or anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1213–1231. doi: 10.1084/jem.151.5.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacobson E. B., Herzenberg L. A. Active suppression of immunoglobulin allotype synthesis. I. Chronic suppression after perinatal exposure to maternal antibody to paternal allotype in (SJL x BALB-c)F 1 mice. J Exp Med. 1972 May 1;135(5):1151–1162. doi: 10.1084/jem.135.5.1151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klinman N. R., Pickard A. R., Sigal N. H., Gearhart P. J., Metcalf E. S., Pierce S. K. Assessing B cell diversification by antigen receptor and precursor cell analysis. Ann Immunol (Paris) 1976 Jun-Jul;127(3-4):489–502. [PubMed] [Google Scholar]
  11. Kuettner M. G., Wang A. L., Nisonoff A. Quantitative investigations of idiotypic antibodies. VI. Idiotypic specificity as a potential genetic marker for the variable regions of mouse immunoglobulin polypeptide chains. J Exp Med. 1972 Mar 1;135(3):579–595. doi: 10.1084/jem.135.3.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mage M. G., McHugh L. L., Rothstein T. L. Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods. 1977;15(1):47–56. doi: 10.1016/0022-1759(77)90016-3. [DOI] [PubMed] [Google Scholar]
  13. Mage R. G. Allotype suppression in rabbits: effects of anti-allotype antisera upon expression of immunoglobulin genes. Transplant Rev. 1975;27:84–99. doi: 10.1111/j.1600-065x.1975.tb00185.x. [DOI] [PubMed] [Google Scholar]
  14. Nelles M. J., Gill-Pazaris L. A., Nisonoff A. Monoclonal ant-idiotypic antibodies reactive with a highly conserved determinant on A/J serum anti-para-azophenylarsonate antibodies. J Exp Med. 1981 Dec 1;154(6):1752–1763. doi: 10.1084/jem.154.6.1752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Owen F. L., Ju S. T., Nisonoff A. Binding to idiotypic determinants of large proportions of thymus-derived lymphocytes in idiotypically suppressed mice. Proc Natl Acad Sci U S A. 1977 May;74(5):2084–2088. doi: 10.1073/pnas.74.5.2084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Owen F. L., Ju S. T., Nisonoff A. Presence on idiotype-specific suppressor T cells of receptors that interact with molecules bearing the idiotype. J Exp Med. 1977 Jun 1;145(6):1559–1566. doi: 10.1084/jem.145.6.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pawlak L. L., Hart D. A., Nisonoff A. Requirements for prolonged suppression of an idiotypic specificity in adult mice. J Exp Med. 1973 Jun 1;137(6):1442–1458. doi: 10.1084/jem.137.6.1442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. I. Stimulation by phytohaemagglutinin. Biochim Biophys Acta. 1970 Dec 29;222(3):565–582. doi: 10.1016/0304-4165(70)90182-0. [DOI] [PubMed] [Google Scholar]
  19. SPIEGELBERG H. L., WEIGLE W. O. THE CATABOLISM OF HOMOLOGOUS AND HETEROLOGOUS 7S GAMMA GLOBULIN FRAGMENTS. J Exp Med. 1965 Mar 1;121:323–338. doi: 10.1084/jem.121.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sy M. S., Dietz M. H., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induce second-order suppressor T cells which express anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1183–1195. doi: 10.1084/jem.151.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thèze J., Waltenbaugh C., Dorf M. E., Benacerraf B. Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyrosine50 (GT) II. Presence of I-J determinants on the GT-suppressive factor. J Exp Med. 1977 Jul 1;146(1):287–292. doi: 10.1084/jem.146.1.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tuffrey M., Bishun N. P., Barnes R. D. Porosity of the mouse placenta to maternal cells. Nature. 1969 Mar 15;221(5185):1029–1030. doi: 10.1038/2211029a0. [DOI] [PubMed] [Google Scholar]
  23. Tung A. S., Ju S. T., Sato S., Nisonoff A. Production of large amounts of antibodies in individual mice. J Immunol. 1976 Mar;116(3):676–681. [PubMed] [Google Scholar]
  24. Weiler I. J., Weiler E., Sprenger R., Cosenza H. Idiotype suppression by maternal influence. Eur J Immunol. 1977 Sep;7(9):591–597. doi: 10.1002/eji.1830070903. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES