Abstract
A plastic adherent variant line (ESb-M) of a highly invasive and metastatic murine T cell lymphoma (ESb) was found to have lost its metastatic potential while still being tumorigenic in normal syngeneic hosts. The variant retained most of its ESb-derived antigenic and biochemical characteristics but differed at binding sites for certain lectins with specificity for terminal N-acetylgalactosamine residues. Whereas such sites were masked by sialic acid on metastatic ESb cells, they became unmasked on the adherent variant line. Metastatic revertants of ESb-M cells did not express the respective lectin receptor sites because these were again masked by sialic acid. It is suggested that the masking of specific lectin receptors sites on the tumor cell surface is of crucial importance for metastatis. If freely exposed, these sites may change adherence characteristics of the cells possibly not only in vitro (to plastic) but also in vivo.
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Selected References
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- Altevogt P., Kurnick J. T., Kimura A. K., Bosslet K., Schirrmacher V. Different expression of Lyt differentiation antigens and cell surface glycoproteins by a murine T lymphoma line and its highly metastatic variant. Eur J Immunol. 1982 Apr;12(4):300–307. doi: 10.1002/eji.1830120409. [DOI] [PubMed] [Google Scholar]
- Dennis J. W., Kerbel R. S. Characterization of a deficiency in fucose metabolism in lectin-resistant variants of a murine tumor showing altered tumorigenic and metastatic capacities in vivo. Cancer Res. 1981 Jan;41(1):98–104. [PubMed] [Google Scholar]
- Hochman J., Katz A., Levy E., Eshel S. Substrate-adhering lymphoid cells show impaired tumorigenicity and increased immunogenicity. Nature. 1981 Mar 19;290(5803):248–249. doi: 10.1038/290248a0. [DOI] [PubMed] [Google Scholar]
- Reading C. L., Belloni P. N., Nicolson G. L. Selection and in vivo properties of lectin-attachment variants of malignant murine lymphosarcoma cell lines. J Natl Cancer Inst. 1980 May;64(5):1241–1249. [PubMed] [Google Scholar]
- Schirrmacher V., Bosslet K. Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice. X. Immunoselection of tumor variants differing in tumor antigen expression and metastatic capacity. Int J Cancer. 1980 Jun 15;25(6):781–788. doi: 10.1002/ijc.2910250614. [DOI] [PubMed] [Google Scholar]
- Schirrmacher V., Cheingsong-Popov R., Arnheiter H. Hepatocyte-tumor cell interaction in vitro. I. Conditions for rosette formation and inhibition by anti-H-2 antibody. J Exp Med. 1980 Apr 1;151(4):984–989. doi: 10.1084/jem.151.4.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schirrmacher V., Fogel M., Russmann E., Bosslet K., Altevogt P., Beck L. Antigenic variation in cancer metastasis: immune escape versus immune control. Cancer Metastasis Rev. 1982;1(3):241–274. doi: 10.1007/BF00046830. [DOI] [PubMed] [Google Scholar]
- Tao T. W., Burger M. M. Non-metastasising variants selected from metastasising melanoma cells. Nature. 1977 Dec 1;270(5636):437–438. doi: 10.1038/270437a0. [DOI] [PubMed] [Google Scholar]
- Yogeeswaran G., Salk P. L. Metastatic potential is positively correlated with cell surface sialylation of cultured murine tumor cell lines. Science. 1981 Jun 26;212(4502):1514–1516. doi: 10.1126/science.7233237. [DOI] [PubMed] [Google Scholar]