Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1983 Mar 1;157(3):947–956. doi: 10.1084/jem.157.3.947

Synthetic Fc peptide-mediated regulation of the immune response. I. Characterization of the immunomodulating properties of a synthetic 23- amino acid peptide derived from the sequence of the CH3 domain of human IgG1

PMCID: PMC2186970  PMID: 6403651

Abstract

A synthetic 23-amino acid peptide derived from the CH3 domain of human IgG1 was found to be a potent adjuvant as well as a polyclonal activator. The Fc peptide was found to enhance human and murine humoral, and T cell-mediated immune responses. Moreover, in vivo administration of Fc peptide enhanced murine natural killer cell activity. The synthetic Fc peptide was found to be more potent, on a molar basis, than native Fc fragments in inducing polyclonal antibody production and potentiating immune responses.

Full Text

The Full Text of this article is available as a PDF (662.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broff M. D., Jonsen M. E., Geha R. S. Nature of the immunogenic moiety recognized by the human T cell proliferating in response to tetanus toxoid antigen. Eur J Immunol. 1981 May;11(5):365–371. doi: 10.1002/eji.1830110504. [DOI] [PubMed] [Google Scholar]
  2. Diener E., Feldmann M. Relationship between antigen and antibody-induced suppression of immunity. Transplant Rev. 1972;8:76–103. doi: 10.1111/j.1600-065x.1972.tb01565.x. [DOI] [PubMed] [Google Scholar]
  3. Edelman G. M., Cunningham B. A., Gall W. E., Gottlieb P. D., Rutishauser U., Waxdal M. J. The covalent structure of an entire gammaG immunoglobulin molecule. Proc Natl Acad Sci U S A. 1969 May;63(1):78–85. doi: 10.1073/pnas.63.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fauci A. S., Whalen G., Burch C. Activation of human B lymphocytes XVI. Cellular requirements, interactions, and immunoregulation of pokeweed mitogen-induced total-immunoglobulin producing plaque-forming cells in peripheral blood. Cell Immunol. 1980 Aug 15;54(1):230–240. doi: 10.1016/0008-8749(80)90204-x. [DOI] [PubMed] [Google Scholar]
  5. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  6. Misiti J., Waldmann T. A. In vitro generation of antigen-specific hemolytic plaque-forming cells from human peripheral blood mononuclear cells. J Exp Med. 1981 Oct 1;154(4):1069–1084. doi: 10.1084/jem.154.4.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morgan E. L., Thoman M. L., Walker S. M., Weigle W. O. Regulation of the immune response. II. Characterization of the cell population(s) involved in the Fc fragment-induced adjuvant effect. J Immunol. 1980 Sep;125(3):1275–1279. [PubMed] [Google Scholar]
  8. Morgan E. L., Thoman M. L., Weigle W. O. Enhancement of T lymphocyte functions by Fc fragments of immunoglobulins. I. Augmentation of allogeneic mixed lymphocyte culture reactions requires I-A- or I-B-subregion differences between effector and stimulator cell populations. J Exp Med. 1981 May 1;153(5):1161–1172. doi: 10.1084/jem.153.5.1161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morgan E. L., Thoman M. L., Weigle W. O. Enhancement of T. lymphocyte functions by Fc fragments of immunoglobulin. II. Augmentation of the cell-mediated lympholysis response occurs through an Lyt-1+2- helper T cell. J Immunol. 1981 Dec;127(6):2526–2530. [PubMed] [Google Scholar]
  10. Morgan E. L., Walker S. M., Thoman M. L., Weigle W. O. Regulation of the immune response. I. The potentiation of in vivo and in vitro immune responses by Fc fragments. J Exp Med. 1980 Jul 1;152(1):113–123. doi: 10.1084/jem.152.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morgan E. L., Weigle W. O. Aggregated human gamma-globulin-induced proliferation and polyclonal activation of murine B lymphocytes. J Immunol. 1980 Jul;125(1):226–231. [PubMed] [Google Scholar]
  12. Morgan E. L., Weigle W. O. Polyclonal activation of human B lymphocytes by Fc fragments. I. Characterization of the cellular requirements for Fc fragment-mediated polyclonal antibody secretion by human peripheral blood B lymphocytes. J Exp Med. 1981 Sep 1;154(3):778–790. doi: 10.1084/jem.154.3.778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morgan E. L., Weigle W. O. Polyclonal activation of murine B lymphocytes by Fc fragments. I. The requirement for two signals in the generation of the polyclonal antibody response induced by Fc fragments. J Immunol. 1980 Mar;124(3):1330–1335. [PubMed] [Google Scholar]
  14. Morgan E. L., Weigle W. O. Regulation of B lymphocyte activation by the Fc portion of immunoglobulin. J Supramol Struct. 1980;14(2):201–208. doi: 10.1002/jss.400140208. [DOI] [PubMed] [Google Scholar]
  15. Morgan E. L., Weigle W. O. Regulation of Fc fragment-induced murine spleen cell proliferation. J Exp Med. 1980 Jan 1;151(1):1–11. doi: 10.1084/jem.151.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morgan E. L., Weigle W. O. The requirement for adherent cells in the Fc fragment-induced proliferative response of murine spleen cells. J Exp Med. 1979 Aug 1;150(2):256–266. doi: 10.1084/jem.150.2.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Möller G., Coutinho A. Role of C'3 and Fc receptors in B-lymphocyte activation. J Exp Med. 1975 Mar 1;141(3):647–663. doi: 10.1084/jem.141.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sjöberg O., Andersson J., Möller G. Lipopolysaccharide can substitute for helper cells in the antibody response in vitro. Eur J Immunol. 1972 Aug;2(4):326–331. doi: 10.1002/eji.1830020406. [DOI] [PubMed] [Google Scholar]
  19. Soderberg L. S., Coons A. H. Complement-dependent stimulation of normal lymphocytes by immune complexes. J Immunol. 1978 Mar;120(3):806–811. [PubMed] [Google Scholar]
  20. Thoman M. L., Morgan E. L., Weigle W. O. Fc fragment activation of T lymphocytes. I. Fc fragments trigger Lyt 1+23- T lymphocytes to release a helper T cell-replacing activity. J Immunol. 1981 Feb;126(2):632–635. [PubMed] [Google Scholar]
  21. Walker J. G., Siskind G. W. Studies on the control of antibody synthesis. Effect of antibody affinity upon its ability to suppress antibody formation. Immunology. 1968 Jan;14(1):21–28. [PMC free article] [PubMed] [Google Scholar]
  22. Yin E. T., Galanos C., Kinsky S., Bradshaw R. A., Wessler S., Lüderitz O., Sarmiento M. E. Picogram-sensitive assay for endotoxin: gelation of Limulus polyphemus blood cell lysate induced by purified lipopolysaccharides and lipid A from Gram-negative bacteria. Biochim Biophys Acta. 1972 Jan 28;261(1):284–289. doi: 10.1016/0304-4165(72)90340-6. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES