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. 1983 Apr 1;157(4):1137–1148. doi: 10.1084/jem.157.4.1137

Surface alterations of erythrocytes in Plasmodium falciparum malaria. Antigenic variation, antigenic diversity, and the role of the spleen

PMCID: PMC2186973  PMID: 6187885

Abstract

The surface of erythrocytes infected with late developmental stages of Plasmodium falciparum is profoundly altered and new antigenic determinants can be detected by surface immunofluorescence using immune squirrel monkey serum. The expression of these parasite-specific antigenic determinants on the surface of the host erythrocyte can be modulated by the presence or absence of the spleen and by immune pressure. An antigenic switch occurred when a cloned population of the Ugandan Palo Alto strain of P. falciparum was transferred from a splenectomized into an intact monkey and this switch was reversible. In another strain (Indochina-1), we showed that the parasites isolated during secondary and recrudescent peaks expressed erythrocyte- associated surface antigens different from the parasites isolated during the primary infection; six variant antigenic types distinct from the original population were isolated in this way. The passive transfer of immune serum can induce antigenic variation and this can occur in a cloned parasite. The various mechanisms of antigenic variation in P. falciparum are discussed in the context of strain-specific diversity and the role of antigenic diversity in acquired immunity.

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Selected References

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  1. BRUCE-CHWATT L. J. A LONGITUDINAL LONGITUDINAL SURVEY OF NATURAL MALARIA INFECTION IN A GROUP OF WEST AFRICAN ADULTS. I. West Afr Med J. 1963 Aug;12:141–173. [PubMed] [Google Scholar]
  2. Barnwell J. W., Howard R. J., Miller L. H. Altered expression of Plasmodium knowlesi variant antigen on the erythrocyte membrane in splenectomized rhesus monkeys. J Immunol. 1982 Jan;128(1):224–226. [PubMed] [Google Scholar]
  3. Brown G. V., Anders R. F., Stace J. D., Alpers M. P., Mitchell G. F. Immunoprecipitation of biosynthetically-labelled proteins from different Papua New Guinea Plasmodium falciparum isolates by sera from individuals in the endemic area. Parasite Immunol. 1981 Winter;3(4):283–298. doi: 10.1111/j.1365-3024.1981.tb00407.x. [DOI] [PubMed] [Google Scholar]
  4. Brown I. N., Brown K. N., Hills L. A. Immunity to malaria: the antibody response to antigenic variation by Plasmodium knowlesi. Immunology. 1968 Jan;14(1):127–138. [PMC free article] [PubMed] [Google Scholar]
  5. Brown K. N., Brown I. N. Immunity to malaria: antigenic variation in chronic infections of Plasmodium knowlesi. Nature. 1965 Dec 25;208(5017):1286–1288. doi: 10.1038/2081286a0. [DOI] [PubMed] [Google Scholar]
  6. David P. H., Hommel M., Oligino L. D. Interactions of Plasmodium falciparum infected erythrocytes with ligand-coated agarose beads. Mol Biochem Parasitol. 1981 Dec;4(3-4):195–204. doi: 10.1016/0166-6851(81)90018-9. [DOI] [PubMed] [Google Scholar]
  7. Diggs C., Joseph K., Flemmings B., Snodgrass R., Hines F. Protein synthesis in vitro by cryopreserved Plasmodium falciparum. Am J Trop Med Hyg. 1975 Sep;24(5):760–763. doi: 10.4269/ajtmh.1975.24.760. [DOI] [PubMed] [Google Scholar]
  8. Gysin J., Hommel M., da Silva L. P. Experimental infection of the squirrel monkey (Saimiri sciureus) with Plasmodium falciparum. J Parasitol. 1980 Dec;66(6):1003–1009. [PubMed] [Google Scholar]
  9. Hommel M., David P. H., Oligino L. D., David J. R. Expression of strain-specific surface antigens on Plasmodium falciparum-infected erythrocytes. Parasite Immunol. 1982 Nov;4(6):409–419. doi: 10.1111/j.1365-3024.1982.tb00452.x. [DOI] [PubMed] [Google Scholar]
  10. Hommel M., David P. H. Plasmodium knowlesi variant antigens are found on schizont-infected erythrocytes but not on merozoites. Infect Immun. 1981 Jul;33(1):275–284. doi: 10.1128/iai.33.1.275-284.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hommel M. Malaria: immunity and prospects for vaccination. West J Med. 1981 Oct;135(4):285–299. [PMC free article] [PubMed] [Google Scholar]
  12. James S. P., Nicol W. D., Shute P. G. A Study of Induced Malignant Tertian Malaria. Proc R Soc Med. 1932 Jun;25(8):1153–1186. [PMC free article] [PubMed] [Google Scholar]
  13. Jeffery G. M. Epidemiological significance of repeated infections with homologous and heterologous strains and species of Plasmodium. Bull World Health Organ. 1966;35(6):873–882. [PMC free article] [PubMed] [Google Scholar]
  14. Kilejian A. Characterization of a protein correlated with the production of knob-like protrusions on membranes of erythrocytes infected with Plasmodium falciparum. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4650–4653. doi: 10.1073/pnas.76.9.4650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Langreth S. G., Reese R. T. Antigenicity of the infected-erythrocyte and merozoite surfaces in Falciparum malaria. J Exp Med. 1979 Nov 1;150(5):1241–1254. doi: 10.1084/jem.150.5.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Luse S. A., Miller L. H. Plasmodium falciparum malaria. Ultrastructure of parasitized erythrocytes in cardiac vessels. Am J Trop Med Hyg. 1971 Sep;20(5):655–660. [PubMed] [Google Scholar]
  17. McBride J. S., Walliker D., Morgan G. Antigenic diversity in the human malaria parasite Plasmodium falciparum. Science. 1982 Jul 16;217(4556):254–257. doi: 10.1126/science.6178159. [DOI] [PubMed] [Google Scholar]
  18. Perkins M. Surface proteins of schizont-infected erythrocytes and merozoites of Plasmodium falciparum. Mol Biochem Parasitol. 1982 Jan;5(1):55–64. doi: 10.1016/0166-6851(82)90006-8. [DOI] [PubMed] [Google Scholar]
  19. Sadun E. H., Hickman R. L., Wellde B. T., Moon A. P., Udeozo I. O. Active and passive immunization of chimpanzees infected with West African and Southeast Asian strains of Plasmodium falciparum. Mil Med. 1966 Sep;131(9 Suppl):1250–1262. [PubMed] [Google Scholar]
  20. Sanderson A., Walliker D., Molez J. F. Enzyme typing of Plasmodium falciparum from African and some other Old World countries. Trans R Soc Trop Med Hyg. 1981;75(2):263–267. doi: 10.1016/0035-9203(81)90331-x. [DOI] [PubMed] [Google Scholar]
  21. Tait A. Analysis of protein variation in Plasmodium falciparum by two-dimensional gel electrophoresis. Mol Biochem Parasitol. 1981 Feb;2(3-4):205–218. doi: 10.1016/0166-6851(81)90101-8. [DOI] [PubMed] [Google Scholar]
  22. Trager W., Rudzinska M. A., Bradbury P. C. The fine structure of Plasmodium falciparum and its host erythrocytes in natural malarial infections in man. Bull World Health Organ. 1966;35(6):883–885. [PMC free article] [PubMed] [Google Scholar]
  23. Van Meirvenne N., Janssens P. G., Magnus E. Antigenic variation in syringe passaged populations of Trypanosoma (Trypanozoon) brucei. 1. Rationalization of the experimental approach. Ann Soc Belg Med Trop. 1975;55(1):1–23. [PubMed] [Google Scholar]
  24. Voller A., Richards W. H. Immunity to Plasmodium falciparum in owl monkeys (Aotus trivirgatus). Z Tropenmed Parasitol. 1970 Jun;21(2):159–166. [PubMed] [Google Scholar]
  25. Wilson R. J. Serotyping Plasmodium falciparum malaria with S-antigens. Nature. 1980 Apr 3;284(5755):451–452. doi: 10.1038/284451a0. [DOI] [PubMed] [Google Scholar]

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