Abstract
The cis-acting gene regulating specifically the inducibility of lambda 1-bearing B cells has been mapped within 2.9 cM of the structural gene. If the lambda 1lo-phenotype is due to the gly leads to val interchange in C lambda 1, then an argument can be made that (a) the lambda 1lo- phenotype is due to inefficient induction of lambda 1lo-bearing B cells and (b) B cell triggering is dependent upon a conformational change in the Ig receptor upon interaction with antigen. If the lambda 1lo- phenotype is due to a regulatory sequence linked to the structural C lambda 1-gene, then it must control the expression of the lambda 1- locus during development into adulthood, e.g., by an effect on methylation.
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Selected References
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- Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Arp B., McMullen M. D., Storb U. Sequences of immunoglobulin lambda 1 genes in a lambda 1 defective mouse strain. Nature. 1982 Jul 8;298(5870):184–187. doi: 10.1038/298184a0. [DOI] [PubMed] [Google Scholar]
- Bailey D. W. Recombinant-inbred strains. An aid to finding identity, linkage, and function of histocompatibility and other genes. Transplantation. 1971 Mar;11(3):325–327. doi: 10.1097/00007890-197103000-00013. [DOI] [PubMed] [Google Scholar]
- Blomberg B., Traunecker A., Eisen H., Tonegawa S. Organization of four mouse lambda light chain immunoglobulin genes. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3765–3769. doi: 10.1073/pnas.78.6.3765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brack C., Hirama M., Lenhard-Schuller R., Tonegawa S. A complete immunoglobulin gene is created by somatic recombination. Cell. 1978 Sep;15(1):1–14. doi: 10.1016/0092-8674(78)90078-8. [DOI] [PubMed] [Google Scholar]
- Cory S., Adams J. M. Deletions are associated with somatic rearrangement of immunoglobulin heavy chain genes. Cell. 1980 Jan;19(1):37–51. doi: 10.1016/0092-8674(80)90386-4. [DOI] [PubMed] [Google Scholar]
- Cotner T., Eisen H. N. The natural abundance of lambda2-light chains in inbred mice. J Exp Med. 1978 Nov 1;148(5):1388–1399. doi: 10.1084/jem.148.5.1388. [DOI] [PMC free article] [PubMed] [Google Scholar]
- D'Eustachio P., Pravtcheva D., Marcu K., Ruddle F. H. Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains. J Exp Med. 1980 Jun 1;151(6):1545–1550. doi: 10.1084/jem.151.6.1545. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elliott B. W., Jr, Eisen H. N., Steiner L. A. Unusual association of V, J and C regions in a mouse immunoglobulin lambda chain. Nature. 1982 Oct 7;299(5883):559–561. doi: 10.1038/299559a0. [DOI] [PubMed] [Google Scholar]
- Geckeler W., Faversham J., Cohn M. On a regulatory gene controlling the expression of the murine lambda1 light chain. J Exp Med. 1978 Nov 1;148(5):1122–1136. doi: 10.1084/jem.148.5.1122. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
- Reilly E. B., Frackelton A. R., Jr, Eisen H. N. Synthesis of lambda 1, lambda 2, and lambda 3 light chains by mouse spleen B cells. Eur J Immunol. 1982 Jul;12(7):552–557. doi: 10.1002/eji.1830120705. [DOI] [PubMed] [Google Scholar]
- Reth M., Imanishi-Kari T., Rajewsky K. Analysis of the repertoire of anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur J Immunol. 1979 Dec;9(12):1004–1013. doi: 10.1002/eji.1830091216. [DOI] [PubMed] [Google Scholar]
- Selsing E., Miller J., Wilson R., Storb U. Evolution of mouse immunoglobulin lambda genes. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4681–4685. doi: 10.1073/pnas.79.15.4681. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Takemori T., Rajewsky K. Lambda chain expression at different stages of ontogeny in C57BL/6, BALB/c and SJL mice. Eur J Immunol. 1981 Aug;11(8):618–625. doi: 10.1002/eji.1830110806. [DOI] [PubMed] [Google Scholar]
- Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weiss S., Lehmann K., Cohn M. Monoclonal antibodies to murine immunoglobulin isotypes. Hybridoma. 1983;2(1):49–54. doi: 10.1089/hyb.1983.2.49. [DOI] [PubMed] [Google Scholar]