Abstract
Spleen cells derived from aged (30 mo) C57Bl/6 mice are shown to be deficient in the ability to generate suppressor cell activity in vitro. The addition of IL-2 to cultures containing aged cells restores this function to a large extent.
Full Text
The Full Text of this article is available as a PDF (351.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anaclerio A., Moras M. L., Honorati C., Ruggeri A., Conti G., Spreafico F. In vitro generation of nonspecific suppressor cells and their characterization. Transplantation. 1979 May;27(5):329–332. doi: 10.1097/00007890-197905000-00008. [DOI] [PubMed] [Google Scholar]
- Bach M. A. Lymphocyte-mediated cytotoxicity: effects of ageing, adult thymectomy and thymic factor. J Immunol. 1977 Aug;119(2):641–647. [PubMed] [Google Scholar]
- DeKruyff R. H., Kim Y. T., Siskind G. W., Weksler M. E. Age related changes in the in vitro immune response: increased suppressor activity in immature and aged mice. J Immunol. 1980 Jul;125(1):142–147. [PubMed] [Google Scholar]
- Doria G., Mancini C., Adorini L. Immunoregulation in senescence: increased inducibility of antigen-specific suppressor T cells and loss of cell sensitivity to immunosuppression in aging mice. Proc Natl Acad Sci U S A. 1982 Jun;79(12):3803–3807. doi: 10.1073/pnas.79.12.3803. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Farrar J. J., Mizel S. B., Fuller-Farrar J., Farrar W. L., Hilfiker M. L. Macrophage-independent activation of helper T cells. I. Production of Interleukin 2. J Immunol. 1980 Aug;125(2):793–798. [PubMed] [Google Scholar]
- Ferguson R. M., Anderson S. M., Simmons R. L. Characterization of suppressor lymphocytes generated in vitro. Transplantation. 1978 Nov;26(5):331–339. doi: 10.1097/00007890-197811000-00013. [DOI] [PubMed] [Google Scholar]
- Gillis S., Baker P. E., Ruscetti F. W., Smith K. A. Long-term culture of human antigen-specific cytotoxic T-cell lines. J Exp Med. 1978 Oct 1;148(4):1093–1098. doi: 10.1084/jem.148.4.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gillis S., Smith K. A. Long term culture of tumour-specific cytotoxic T cells. Nature. 1977 Jul 14;268(5616):154–156. doi: 10.1038/268154a0. [DOI] [PubMed] [Google Scholar]
- Goidl E. A., Innes J. B., Weksler M. E. Immunological studies of aging. II. Loss of IgG and high avidity plaque-forming cells and increased suppressor cell activity in aging mice. J Exp Med. 1976 Oct 1;144(4):1037–1048. doi: 10.1084/jem.144.4.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green D. R., Gershon R. K., Eardley D. D. Functional deletion of different Ly-1 T-cell-inducer subset activities by Ly-2 suppressor T lymphocytes. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3819–3823. doi: 10.1073/pnas.78.6.3819. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hallgren H. M., Yunis E. J. Suppressor lymphocytes in young and aged humans. J Immunol. 1977 Jun;118(6):2004–2008. [PubMed] [Google Scholar]
- Heidrick M. L., Makinodan T. Presence of impairment of humoral immunity in nonadherent spleen cells of old mice. J Immunol. 1973 Nov;111(5):1502–1506. [PubMed] [Google Scholar]
- Hodes R. J., Hathcock K. S. In vitro generation of suppressor cell activity: suppression of in vitro induction if cell-mediated cytotoxicity. J Immunol. 1976 Jan;116(1):167–177. [PubMed] [Google Scholar]
- Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
- Kay M. M., Makinodan T. Relationship between aging and immune system. Prog Allergy. 1981;29:134–181. [PubMed] [Google Scholar]
- Konen T. G., Smith G. S., Walford R. L. Decline in mixed lymphocyte reactivity of spleen cells from aged mice of a long-lived strain. J Immunol. 1973 May;110(5):1216–1221. [PubMed] [Google Scholar]
- Krogsrud R. L., Perkins E. H. Age-related changes in T cell function. J Immunol. 1977 May;118(5):1607–1611. [PubMed] [Google Scholar]
- Liu J. J., Segre M., Segre D. Changes in suppressor, helper, and B-cell functions in aging mice. Cell Immunol. 1982 Jan 15;66(2):372–382. doi: 10.1016/0008-8749(82)90187-3. [DOI] [PubMed] [Google Scholar]
- Makinodan T., Kay M. M. Age influence on the immune system. Adv Immunol. 1980;29:287–330. doi: 10.1016/s0065-2776(08)60047-4. [DOI] [PubMed] [Google Scholar]
- Miller R. A., Stutman O. Decline, in aging mice, of the anti-2,4,6-trinitrophenyl (TNP) cytotoxic T cell response attributable to loss of Lyt-2-, interleukin 2-producing helper cell function. Eur J Immunol. 1981 Oct;11(10):751–756. doi: 10.1002/eji.1830111004. [DOI] [PubMed] [Google Scholar]
- Morgan E. L., Weigle W. O. The immune response in aged C57BL/6 mice. II. Characterization and reversal of a defect in the ability of aged spleen cells to respond to the adjuvant properties of Fc fragments. J Immunol. 1982 Jul;129(1):36–39. [PubMed] [Google Scholar]
- Roder J. C., Bell D. A., Singhal S. K. Regulation of the immune response in autoimmune NZB/NZW F1 mice. I. The spontaneous generation of splenic suppressor cells. Cell Immunol. 1977 Mar 15;29(2):272–284. doi: 10.1016/0008-8749(77)90322-7. [DOI] [PubMed] [Google Scholar]
- Segre D., Segre M. Humoral immunity in aged mice. II. Increased suppressor T cell activity in immunologically deficient old mice. J Immunol. 1976 Mar;116(3):735–738. [PubMed] [Google Scholar]
- Thoman M. L., Weigle W. O. Cell-mediated immunity in aged mice: an underlying lesion in IL 2 synthesis. J Immunol. 1982 May;128(5):2358–2361. [PubMed] [Google Scholar]
- Thoman M. L., Weigle W. O. Lymphokines and aging: interleukin-2 production and activity in aged animals. J Immunol. 1981 Nov;127(5):2102–2106. [PubMed] [Google Scholar]
- Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J Exp Med. 1979 Dec 1;150(6):1510–1519. doi: 10.1084/jem.150.6.1510. [DOI] [PMC free article] [PubMed] [Google Scholar]