Abstract
Peripheral T lymphocytes activated in vitro with concanavalin A (Con A) or alloantigens express the thymus leukemia (TL) alloantigen as assessed by staining with the monoclonal antibody TL.m3 and flow cytometric analysis. The determinants detected by TL.m3 on activated cells are encoded within the Tla region and are detected as early as 48 h after activation with Con A. Several long-term cloned cytotoxic T lymphocyte lines were also examined and each expressed TL. By two- dimensional analysis, the TL isolated from activated peripheral cells was indistinguishable from that found on thymocytes and the leukemia cell line ASL-1.
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- Cook R. G., Jenkins R. N., Flaherty L., Rich R. R. The Qa-1 alloantigens. II. Evidence for the expression of two Qa-1 molecules by the Qa-1d genotype and for cross-reactivity between Qa-1 and H-2K. J Immunol. 1983 Mar;130(3):1293–1299. [PubMed] [Google Scholar]
- Goodenow R. S., McMillan M., Nicolson M., Sher B. T., Eakle K., Davidson N., Hood L. Identification of the class I genes of the mouse major histocompatibility complex by DNA-mediated gene transfer. Nature. 1982 Nov 18;300(5889):231–237. doi: 10.1038/300231a0. [DOI] [PubMed] [Google Scholar]
- Kappler J. W., Skidmore B., White J., Marrack P. Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J Exp Med. 1981 May 1;153(5):1198–1214. doi: 10.1084/jem.153.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
- King D. P., Strober S., Kaplan H. S. Immunoregulatory changes induced by total lymphoid irradiation (TLI). I. Appearance of a population of cells bearing the thymus leukemia (TL) surface antigen in the lymph nodes and spleen. J Immunol. 1981 Sep;127(3):1085–1089. [PubMed] [Google Scholar]
- Lemke H., Hämmerling G. J., Hämmerling U. Fine specificity analysis with monoclonal antibodies of antigens controlled by the major histocompatibility complex and by the Qa/TL region in mice. Immunol Rev. 1979;47:175–206. doi: 10.1111/j.1600-065x.1979.tb00293.x. [DOI] [PubMed] [Google Scholar]
- McIntyre K. R., Hämmerling U., Uhr J. W., Vitetta E. S. Structural analysis of thymus-leukemia (TL) antigens in the mouse. J Immunol. 1982 Apr;128(4):1712–1717. [PubMed] [Google Scholar]
- Michaelson J., Tung J. S., Flaherty L., Hämmerling U., Bushkin Y. The biochemical genetics of TL antigens on mouse thymocytes. Eur J Immunol. 1982 Apr;12(4):257–261. doi: 10.1002/eji.1830120402. [DOI] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Pure E., Isakson P. C., Kappler J. W., Marrack P., Krammer P. H., Vitetta E. S. T cell-derived B cell growth and differentiation factors. Dichotomy between the responsiveness of B cells from adult and neonatal mice. J Exp Med. 1983 Feb 1;157(2):600–612. doi: 10.1084/jem.157.2.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ranges G. E., Goldstein G., Boyse E. A., Schield M. P. T cell development in normal and thymopentin-treated nude mice. J Exp Med. 1982 Oct 1;156(4):1057–1064. doi: 10.1084/jem.156.4.1057. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothenberg E., Boyse E. A. Synthesis and processing of molecules bearing thymus leukemia antigen. J Exp Med. 1979 Oct 1;150(4):777–791. doi: 10.1084/jem.150.4.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scollay R., Jacobs S., Jerabek L., Butcher E., Weissman I. T cell maturation: thymocyte and thymus migrant subpopulations defined with monoclonal antibodies to MHC region antigens. J Immunol. 1980 Jun;124(6):2845–2853. [PubMed] [Google Scholar]
- Shen F. W., Chorney M. J., Boyse E. A. Further polymorphism of the Tla locus defined by monoclonal TL antibodies. Immunogenetics. 1982;15(6):573–578. doi: 10.1007/BF00347051. [DOI] [PubMed] [Google Scholar]
- Wysocki L. J., Sato V. L. "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2844–2848. doi: 10.1073/pnas.75.6.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yokoyama K., Stockert E., Old L. J., Nathenson S. G. Structural comparisons of TL antigens derived from normal and leukemia cells of Tl+ and TL- strains and relationship to genetically linked H-2 major histocompatibility complex products. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7078–7082. doi: 10.1073/pnas.78.11.7078. [DOI] [PMC free article] [PubMed] [Google Scholar]