Abstract
The expression of HLA-DC/DS antigen detected by the monoclonal antibody Leu 10 was studied in three human precursor and pre-B cell lines (Josh 7, Reh, and Nalm 12). Flow cytometric analysis showed that none of these cell lines stained for the HLA-DC/DS antigen. In the presence of 1.6 X 10(-9) M of 12-O-tetradecanoylporbol-13-acetate (TPA), expression of this antigen was detected. The expression was completed after 168 h of incubation. Iodination of cell surface, immunoprecipitation by Leu 10 antibody, and two-dimensional gel analysis revealed that TPA-treated Josh 7 cells synthesized and expressed a 29,34 kD bimolecular complex with both alpha and beta chains different from those of HLA-DR antigen. Quantitative absorption experiments with cell lysates indicated a greater than 25-fold increase in HLA-DC/DS antigen in TPA-treated cells. With the induction of HLA-DC/DS antigen expression, there are concomitant decreases in the expression of the common acute lymphoblastic leukemia antigen (CALLA) and the enzymatic activity of terminal deoxynucleotidyl transferase. No appreciable changes in HLA-DR and Ig expression were observed. There was also no change in HLA-SB expression as detected by antibody ILR-1. However, DNA synthesis was markedly inhibited by TPA treatment. These results indicate that precursor and pre-B cell lines can be induced to mature in vitro. They also suggest that the expression of HLA-DC/DS antigen which precedes the expression of membrane Ig and follows the HLA-DR expression is relevant to human B cell development and cell interaction.
Full Text
The Full Text of this article is available as a PDF (517.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brodsky F. M., Parham P., Bodmer W. F. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens. 1980 Jul;16(1):30–48. doi: 10.1111/j.1399-0039.1980.tb00285.x. [DOI] [PubMed] [Google Scholar]
- Diamond L., O'Brien T. G., Baird W. M. Tumor promoters and the mechanism of tumor promotion. Adv Cancer Res. 1980;32:1–74. doi: 10.1016/s0065-230x(08)60360-7. [DOI] [PubMed] [Google Scholar]
- Fu S. M., Hurley J. N. Human cell lines containing Epstein-Barr virus but distinct from the common B cell lymphoblastoid lines. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6637–6640. doi: 10.1073/pnas.76.12.6637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goyert S. M., Shively J. E., Silver J. Biochemical characterization of a second family of human Ia molecules, HLA-DS, equivalent to murine I-A subregion molecules. J Exp Med. 1982 Aug 1;156(2):550–566. doi: 10.1084/jem.156.2.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LeBien T. W., Bollum F. J., Yasmineh W. G., Kersey J. H. Phorbol ester-induced differentiation of a non-T, non-B leukemic cell line: model for human lymphoid progenitor cell development. J Immunol. 1982 Mar;128(3):1316–1320. [PubMed] [Google Scholar]
- Minowada J., Koshiba H., Sagawa K., Kubonishi I., Lok M. S., Tatsumi E., Han T., Srivastava B. I., Ohnuma T. Marker profiles of human leukemia and lymphoma cell lines. J Cancer Res Clin Oncol. 1981;101(1):91–100. doi: 10.1007/BF00405069. [DOI] [PubMed] [Google Scholar]
- Nadler L. M., Ritz J., Bates M. P., Park E. K., Anderson K. C., Sallan S. E., Schlossman S. F. Induction of human B cell antigens in non-T cell acute lymphoblastic leukemia. J Clin Invest. 1982 Aug;70(2):433–442. doi: 10.1172/JCI110633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nadler L. M., Stashenko P., Hardy R., Tomaselli K. J., Yunis E. J., Schlossman S. F., Pesando J. M. Monoclonal antibody identifies a new Ia-like (p29,34) polymorphic system linked to the HLA-D/DR region. Nature. 1981 Apr 16;290(5807):591–593. doi: 10.1038/290591a0. [DOI] [PubMed] [Google Scholar]
- Newman R. A., Delia D., Greaves M. F., Navarrete C., Fainboim L., Festenstein H. Differential expression of HLA-DR and DR-linked determinants on human leukemias and lymphoid cells. Eur J Immunol. 1983 Feb;13(2):172–176. doi: 10.1002/eji.1830130215. [DOI] [PubMed] [Google Scholar]
- O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
- Shackelford D. A., Mann D. L., van Rood J. J., Ferrara G. B., Strominger J. L. Human B-cell alloantigens DC1, MT1, and LB12 are identical to each other but distinct from the HLA-DR antigen. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4566–4570. doi: 10.1073/pnas.78.7.4566. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sieff C., Bicknell D., Caine G., Robinson J., Lam G., Greaves M. F. Changes in cell surface antigen expression during hemopoietic differentiation. Blood. 1982 Sep;60(3):703–713. [PubMed] [Google Scholar]
- Tötterman T. H., Nilsson K., Sundström C. Phorbol ester-induced differentiation of chronic lymphocytic leukaemia cells. Nature. 1980 Nov 13;288(5787):176–178. doi: 10.1038/288176a0. [DOI] [PubMed] [Google Scholar]
- Wang C. Y., Fu S. M., Kunkel H. G. Isolation and immunological characterization of a major surface glycoprotein (gp54) preferentially expressed on certain human B cells. J Exp Med. 1979 Jun 1;149(6):1424–1437. doi: 10.1084/jem.149.6.1424. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winchester R. J., Kunkel H. G. The human Ia system. Adv Immunol. 1979;28:221–292. [PubMed] [Google Scholar]
- Yasmineh W. G., Smith B. M., Bloomfield C. D. DNA nucleotidylexotransferase of normal persons and leukemic patients. Clin Chem. 1980 Jun;26(7):891–895. [PubMed] [Google Scholar]