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. 1983 Nov 1;158(5):1444–1458. doi: 10.1084/jem.158.5.1444

A cloned major histocompatibility complex-restricted trinitrophenyl- reactive human helper T cell line that activates B cell subsets via two distinct pathways

PMCID: PMC2187142  PMID: 6195284

Abstract

A cloned, trinitrophenyl (TNP)-specific helper T cell line (TCL), termed E-11, has been established in long-term, interleukin 2-dependent culture and used to study human T helper (Th)-B cell collaboration. Co- culture of E-11 with TNP-modified, but not unmodified or FITC-modified, autologous B cells results in a vigorous, polyclonally plaque-forming cell (PFC) response. E-11 helper activity is not constitutive, but requires antigen-specific, major histocompatibility complex-restricted activation of the TCL cells by interaction with TNP-modified autologous or DR 5+ allogeneic macrophages. Using B cell subsets isolated by discontinuous density gradient cengrifugation as responder populations, we determined that E-11 activates B cell subsets via two distinct mechanisms: (a) E-11 polyclonally activates large B cells in an unrestricted and nonspecific manner; and (b) E-11 preferentially induces a PFC response by TNP-modified small B cells. These results suggest that the large B cell subset is activated by helper signals generated during the Th-antigen-presenting cell interaction, while small B cells require an additional stimulus that is provided by antigen-specific Th-B cell contact.

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Selected References

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  1. Andersson J., Schreier M. H., Melchers F. T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1612–1616. doi: 10.1073/pnas.77.3.1612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Asano Y., Shigeta M., Fathman C. G., Singer A., Hodes R. J. Role of the major histocompatibility complex in T cell activation of B cell subpopulations. A single monoclonal T helper cell population activates different B cell subpopulations by distinct pathways. J Exp Med. 1982 Aug 1;156(2):350–360. doi: 10.1084/jem.156.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Breard J., Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol. 1980 Apr;124(4):1943–1948. [PubMed] [Google Scholar]
  4. Friedman S. M., Hunter S. B., Thomas Y., Chess L. Hapten-specific human T cell lines. I. Functional analysis with respect to cytotoxic and helper activity. J Immunol. 1980 Nov;125(5):2202–2207. [PubMed] [Google Scholar]
  5. Friedman S. M., Principato M. A., Thompson G. S., Teichman F. Antigen-specific and polyclonal immunoglobulin production induced by a cloned tetanus toxoid-specific T cell line. J Immunol. 1983 Mar;130(3):1164–1170. [PubMed] [Google Scholar]
  6. Friedman S. M., Thompson G. S., Principato M. A. Allospecific human T cell lines and clones which mediate HLA-DR restricted helper activity. J Immunol. 1982 Dec;129(6):2451–2457. [PubMed] [Google Scholar]
  7. Friedman S. M., Thompson G., Halper J. P., Knowles D. M. OT-CLL: a human T cell chronic lymphocytic leukemia that produces IL 2 in high titer. J Immunol. 1982 Feb;128(2):935–940. [PubMed] [Google Scholar]
  8. Gleichmann E., Van Elven E. H., Van der Veen J. P. A systemic lupus erythematosus (SLE)-like disease in mice induced by abnormal T-B cell cooperation. Preferential formation of autoantibodies characteristic of SLE. Eur J Immunol. 1982 Feb;12(2):152–159. doi: 10.1002/eji.1830120210. [DOI] [PubMed] [Google Scholar]
  9. Julius M. H., von Boehmer H., Sidman C. L. Dissociation of two signals required for activation of resting B cells. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1989–1993. doi: 10.1073/pnas.79.6.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keightley R. G., Cooper M. D., Lawton A. R. The T cell dependence of B cell differentiation induced by pokeweed mitogen. J Immunol. 1976 Nov;117(5 Pt 1):1538–1544. [PubMed] [Google Scholar]
  11. Kung P., Goldstein G., Reinherz E. L., Schlossman S. F. Monoclonal antibodies defining distinctive human T cell surface antigens. Science. 1979 Oct 19;206(4416):347–349. doi: 10.1126/science.314668. [DOI] [PubMed] [Google Scholar]
  12. Kuritani T., Cooper M. D. Human B cell differentiation. II. Pokeweed mitogen-responsive B cells belong to a surface immunoglobulin D-negative subpopulation. J Exp Med. 1982 May 1;155(5):1561–1566. doi: 10.1084/jem.155.5.1561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lucivero G., Lawton A. R., Cooper M. D. Rosette formation with mouse erythrocytes defines a population of human B lymphocytes unresponsive to pokeweed mitogen. Clin Exp Immunol. 1981 Jul;45(1):185–190. [PMC free article] [PubMed] [Google Scholar]
  14. Muraguchi A., Butler J. L., Kehrl J. H., Fauci A. S. Differential sensitivity of human B cell subsets to activation signals delivered by anti-mu antibody and proliferative signals delivered by a monoclonal B cell growth factor. J Exp Med. 1983 Feb 1;157(2):530–546. doi: 10.1084/jem.157.2.530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pettersson S., Pobor G., Coutinho A. MHC restriction of male-antigen-specific T helper cells collaborating in antibody responses. Immunogenetics. 1982;15(2):129–138. doi: 10.1007/BF00621946. [DOI] [PubMed] [Google Scholar]
  16. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol. 1980 Mar;124(3):1301–1307. [PubMed] [Google Scholar]
  17. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. Further characterization of the human inducer T cell subset defined by monoclonal antibody. J Immunol. 1979 Dec;123(6):2894–2896. [PubMed] [Google Scholar]
  18. Reinherz E. L., Kung P. C., Pesando J. M., Ritz J., Goldstein G., Schlossman S. F. Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression. J Exp Med. 1979 Dec 1;150(6):1472–1482. doi: 10.1084/jem.150.6.1472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rolink A. G., Radaszkiewicz T., Pals S. T., van der Meer W. G., Gleichmann E. Allosuppressor and allohelper T cells in acute and chronic graft-vs-host disease. I. Alloreactive suppressor cells rather than killer T cells appear to be the decisive effector cells in lethal graft-vs.-host disease. J Exp Med. 1982 May 1;155(5):1501–1522. doi: 10.1084/jem.155.5.1501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shulman H. M., Sullivan K. M., Weiden P. L., McDonald G. B., Striker G. E., Sale G. E., Hackman R., Tsoi M. S., Storb R., Thomas E. D. Chronic graft-versus-host syndrome in man. A long-term clinicopathologic study of 20 Seattle patients. Am J Med. 1980 Aug;69(2):204–217. doi: 10.1016/0002-9343(80)90380-0. [DOI] [PubMed] [Google Scholar]
  21. Verbi W., Greaves M. F., Schneider C., Koubek K., Janossy G., Stein H., Kung P., Goldstein G. Monoclonal antibodies OKT 11 and OKT 11A have pan-T reactivity and block sheep erythrocyte "receptors". Eur J Immunol. 1982 Jan;12(1):81–86. doi: 10.1002/eji.1830120115. [DOI] [PubMed] [Google Scholar]
  22. Zubler R. H., Kanagawa O. Requirement for three signals in B cell responses. II. Analysis of antigen- and Ia-restricted T helper cell-B cell interaction. J Exp Med. 1982 Aug 1;156(2):415–429. doi: 10.1084/jem.156.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

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