Abstract
We describe a radioimmunoassay for rabbit C5a and its use to obtain evidence of extravascular C5a generation in two inflammatory reactions in the peritoneal cavity. These observations, together with the potent activity of C5a in inducing increased microvascular permeability involving circulating PMN leukocytes, strengthen the case for considering C5a an important inflammatory mediator. These findings offer an explanation for the many different experimental inflammatory reactions where oedema formation can be suppressed either by systemic depletion of complement or by depletion of circulating PMN leukocytes.
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Selected References
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- Fantone J. C., Kunkel S. L., Ward P. A. Chemotactic mediators in neutrophil-dependent lung injury. Annu Rev Physiol. 1982;44:283–293. doi: 10.1146/annurev.ph.44.030182.001435. [DOI] [PubMed] [Google Scholar]
- Fernandez H. N., Hugli T. E. Primary structural analysis of the polypeptide portion of human C5a anaphylatoxin. Polypeptide sequence determination and assignment of the oligosaccharide attachment site in C5a. J Biol Chem. 1978 Oct 10;253(19):6955–6964. [PubMed] [Google Scholar]
- Johnson A. R., Hugli T. E., Müller-Eberhard H. J. Release of histamine from rat mast cells by the complement peptides C3a and C5a. Immunology. 1975 Jun;28(6):1067–1080. [PMC free article] [PubMed] [Google Scholar]
- Orr F. W., Varani J., Kreutzer D. L., Senior R. M., Ward P. A. Digestion of the fifth component of complement by leukocyte enzymes. Sequential generation of chemotactic activities for leukocytes and for tumor cells. Am J Pathol. 1979 Jan;94(1):75–83. [PMC free article] [PubMed] [Google Scholar]
- Ward P. A., Hill J. H. Biologic role of complement products. Complement-derived leukotactic activity extractable from lesions of immunologic vasculitis. J Immunol. 1972 May;108(5):1137–1145. [PubMed] [Google Scholar]
- Wedmore C. V., Williams T. J. Control of vascular permeability by polymorphonuclear leukocytes in inflammation. Nature. 1981 Feb 19;289(5799):646–650. doi: 10.1038/289646a0. [DOI] [PubMed] [Google Scholar]
- Williams T. J., Jose P. J. Mediation of increased vascular permeability after complement activation. Histamine-independent action of rabbit C5a. J Exp Med. 1981 Jan 1;153(1):136–153. doi: 10.1084/jem.153.1.136. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams T. J., Peck M. J. Role of prostaglandin-mediated vasodilatation in inflammation. Nature. 1977 Dec 8;270(5637):530–532. doi: 10.1038/270530a0. [DOI] [PubMed] [Google Scholar]