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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1984 Jan 1;159(1):103–113. doi: 10.1084/jem.159.1.103

Physiology of IgD. IV. Enhancement of antibody production in mice bearing IgD-secreting plasmacytomas

PMCID: PMC2187210  PMID: 6363593

Abstract

Immune responses to trinitrophenylated hemocyanin (TNP-KLH), Ficoll (TNP-Ficoll), and Brucella abortus (TNP-BA) were examined in BALB/c mice bearing subcutaneous transplants of TEPC-1017 and TEPC-1033, the two known IgD-secreting BALB/c plasmacytomas. Both primary and secondary 19S and 7S splenic plaque-forming cell (PFC) responses in spleen to intravenously injected TNP-KLH were enhanced three to fivefold. Primary responses to TNP-Ficoll were 1.5-2 times higher than in control mice (particularly the 7S PFC response). Primary responses to TNP-BA were enhanced by TEPC-1017 but suppressed by TEPC-1033, while secondary responses to TNP-BA were enhanced three to sevenfold by both tumors. Intraperitoneal injections of ascites fluid from mice bearing TEPC-1017 or TEPC-1033, or of IgD isolated from such ascites fluid, caused a similar enhancement of the primary response to TNP-KLH, as did the tumor itself, particularly when injected approximately 1 wk before antigen injection. IgD-containing ascites fluid had no effect on the response of athymic (nu/nu) BALB/c mice to TNP-KLH. These findings suggest the existence of an IgD-responsive immunoregulatory T cell.

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Selected References

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  1. Fridman W. H., Rabourdin-Combe C., Neauport-Sautes C., Gisler R. H. Characterization and function of T cell Fc gamma receptor. Immunol Rev. 1981;56:51–88. doi: 10.1111/j.1600-065x.1981.tb01047.x. [DOI] [PubMed] [Google Scholar]
  2. Fridman W. H., Rabourdin-Combe C., Neauport-Sautes C., Gisler R. H. Characterization and function of T cell Fc gamma receptor. Immunol Rev. 1981;56:51–88. doi: 10.1111/j.1600-065x.1981.tb01047.x. [DOI] [PubMed] [Google Scholar]
  3. Goidl E. A., Romano T. J., Siskind G. W., Thorbecke G. J. Changes in affinity of 19 and 7S antibodies at the cellular level in responses to hapten conjugates of varying T dependency. Cell Immunol. 1978 Feb;35(2):231–241. doi: 10.1016/0008-8749(78)90145-4. [DOI] [PubMed] [Google Scholar]
  4. Herzenberg L. A. Epitope-specific regulation of memory B-cell expression. Ann N Y Acad Sci. 1982;399:368–374. doi: 10.1111/j.1749-6632.1982.tb25690.x. [DOI] [PubMed] [Google Scholar]
  5. Hirano T., Kumagai Y., Okumura K., Ovary Z. Regulation of murine IgE production: importance of a not-yet-described T cell for IgE secretion demonstrated in SJA9 mice. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3435–3438. doi: 10.1073/pnas.80.11.3435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hoover R. G., Lynch R. G. Lymphocyte surface membrane immunoglobulin in myeloma. II. T cells with IgA-Fc receptors are markedly increased in mice with IgA plasmacytomas. J Immunol. 1980 Sep;125(3):1280–1288. [PubMed] [Google Scholar]
  7. Jacobson E. B., Baine Y., Chen Y. W., Flotte T., O'Neil M. J., Pernis B., Siskind G. W., Thorbecke G. J., Tonda P. Physiology of IgD. I. Compensatory phenomena in B lymphocyte activation in mice treated with anti-IgD antibodies. J Exp Med. 1981 Aug 1;154(2):318–332. doi: 10.1084/jem.154.2.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jacobson E. B., Caporale L. H., Thorbecke G. J. Effect of thymus cell injections on germinal center formation in lymphoid tissues of nude (thymusless) mice. Cell Immunol. 1974 Sep;13(3):416–430. doi: 10.1016/0008-8749(74)90261-5. [DOI] [PubMed] [Google Scholar]
  9. Kanowith-Klein S., Vitetta E. S., Korn E. L., Ashman R. F. Antigen-induced changes in the proportion of antigen-binding cells expressing IgM, IgG, and IgD receptors. J Immunol. 1979 Jun;122(6):2349–2355. [PubMed] [Google Scholar]
  10. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Monroe J. G., Havran W. L., Cambier J. C. B lymphocyte activation: entry into cell cycle is accompanied by decreased expression of IgD but not IgM. Eur J Immunol. 1983 Mar;13(3):208–213. doi: 10.1002/eji.1830130306. [DOI] [PubMed] [Google Scholar]
  12. Moretta L., Ferrarini M., Durante M. L., Mingari M. C. Expression of a receptor for IgM by human T cells in vitro. Eur J Immunol. 1975 Aug;5(8):565–569. doi: 10.1002/eji.1830050812. [DOI] [PubMed] [Google Scholar]
  13. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nagylaki T. Random genetic drift in a cline. Proc Natl Acad Sci U S A. 1978 Jan;75(1):423–426. doi: 10.1073/pnas.75.1.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  16. Sidman C. L., Unanue E. R. Control of proliferation and differentiation in B lymphocytes by anti-Ig antibodies and a serum-derived cofactor. Proc Natl Acad Sci U S A. 1978 May;75(5):2401–2405. doi: 10.1073/pnas.75.5.2401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ullrich S., Zolla-Pazner S. Immunoregulatory circuits in myeloma. Clin Haematol. 1982 Feb;11(1):87–111. [PubMed] [Google Scholar]
  18. Vitetta E., Puré E., Isakson P., Buck L., Uhr J. The activation of murine B cells: the role of surface immunoglobulins. Immunol Rev. 1980;52:211–231. doi: 10.1111/j.1600-065x.1980.tb00336.x. [DOI] [PubMed] [Google Scholar]
  19. Yoshida T. O., Andersson B. Evidence for a receptor recognizing antigen complexed immunoglobulin on the surface of activated mouse thymus lymphocytes. Scand J Immunol. 1972;1(4):401–408. doi: 10.1111/j.1365-3083.1972.tb03306.x. [DOI] [PubMed] [Google Scholar]
  20. Zolla-Pazner S., Sullivan B., Richardson D. Cellular specificity of plasmacytoma-induced immunosuppression. J Immunol. 1976 Aug;117(2):563–568. [PubMed] [Google Scholar]
  21. Zolla S. The effect of plasmacytomas on the immune response of mice. J Immunol. 1972 Apr;108(4):1039–1048. [PubMed] [Google Scholar]

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