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. 1984 Feb 1;159(2):635–640. doi: 10.1084/jem.159.2.635

Sequence of a cDNA encoding Basilea kappa light chains (K2 isotype) suggests a possible relationship of protein structure to limited expression

PMCID: PMC2187219  PMID: 6420504

Abstract

We present the complete sequence of a cDNA encoding rabbit immunoglobulin kappa light chains of the Basilea isotype (K2). Although all rabbits seem to possess a K2 constant region gene, expression of this gene in most rabbits is minimal if present at all. Even in Basilea rabbits the majority of expressed immunoglobulins are of lambda type. We find that the sequence of our Basilea cDNA constant region and the sequence of a "silent" K2 gene from b4 rabbits (bas-N4) are almost identical. The bas (K2) isotype lacks cysteine at position 171 in the constant region that is present in all K1 constant regions and usually forms an interdomain disulfide bond, with a cysteine at position 80 of the variable region. We postulate that one factor contributing to the low expression of the bas (K2) isotype could be a paucity of V kappa regions lacking cysteine at position 80. If a typical rabbit V kappa encoding Cys at position 80 is rearranged and expressed with th K2 isotype. B cells with mRNAs encoding light chains with free sulfhydryl groups would result. These cells may fail to form functional immunoglobulin receptors. Only a small subset of rabbit variable regions that lack the cysteine at position 80 would rearrange and encode K2 light chains lacking a free sulfhydryl group.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arp B., McMullen M. D., Storb U. Sequences of immunoglobulin lambda 1 genes in a lambda 1 defective mouse strain. Nature. 1982 Jul 8;298(5870):184–187. doi: 10.1038/298184a0. [DOI] [PubMed] [Google Scholar]
  2. Benammar A., Cazenave P. A. A second rabbit kappa isotype. J Exp Med. 1982 Aug 1;156(2):585–595. doi: 10.1084/jem.156.2.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bernstein K. E., Pavirani A., Alexander C., Jacobsen F., Fitzmaurice L., Mage R. Use of Trypanosoma equiperdum infected rabbits as a source of splenic mRNA; construction of cDNA clones and identification of a rabbit mu heavy chain clone. Mol Immunol. 1983 Jan;20(1):89–99. doi: 10.1016/0161-5890(83)90108-6. [DOI] [PubMed] [Google Scholar]
  4. Bernstein K. E., Skurla R. M., Jr, Mage R. G. The sequences of rabbit kappa light chains of b4 and b5 allotypes differ more in their constant regions than in their 3' untranslated regions. Nucleic Acids Res. 1983 Oct 25;11(20):7205–7214. doi: 10.1093/nar/11.20.7205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Emorine L., Dreher K., Kindt T. J., Max E. E. Rabbit immunoglobulin kappa genes: structure of a germline b4 allotype J-C locus and evidence for several b4-related sequences in the rabbit genome. Proc Natl Acad Sci U S A. 1983 Sep;80(18):5709–5713. doi: 10.1073/pnas.80.18.5709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Garcia I., Brandt D. C., Benammar A., Cazenave P. A., Jaton J. C. BASILEA rabbits express two types of immunoglobulin light chains: lambda and kappa-like. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4391–4394. doi: 10.1073/pnas.79.14.4391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Good P. W., Notenboom R., Dubiski S., Cinader B. Basilea rabbit immunoglobulins: detection and characterization by specific alloantiserum. J Immunol. 1980 Sep;125(3):1293–1297. [PubMed] [Google Scholar]
  8. Heidmann O., Auffray C., Cazenave P. A., Rougeon F. Nucleotide sequence of constant and 3' untranslated regions of a kappa immunoglobulin light chain mRNA of a homozygous b4 rabbit. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5802–5806. doi: 10.1073/pnas.78.9.5802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Heidmann O., Rougeon F. Multiple sequences related to a constant-region kappa light chain gene in the rabbit genome. Cell. 1982 Mar;28(3):507–513. doi: 10.1016/0092-8674(82)90205-7. [DOI] [PubMed] [Google Scholar]
  10. Heidmann O., Rougeon F. Multiplicity of constant kappa light chain genes in the rabbit genome: a b4b4 homozygous rabbit contains a kappa-bas gene. EMBO J. 1983;2(3):437–441. doi: 10.1002/j.1460-2075.1983.tb01441.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hutchison K. W., Halvorson H. O. Cloning of randomly sheared DNA fragments from a phi 105 lysogen of Bacillus subtilis identification of prophage-containing clones. Gene. 1980 Feb;8(3):267–278. doi: 10.1016/0378-1119(80)90004-9. [DOI] [PubMed] [Google Scholar]
  12. Kelus A. S., Weiss S. Variant strain of rabbits lacking immunoglobulin kappa polypeptide chain. Nature. 1977 Jan 13;265(5590):156–158. doi: 10.1038/265156a0. [DOI] [PubMed] [Google Scholar]
  13. Kwan S. P., Max E. E., Seidman J. G., Leder P., Scharff M. D. Two kappa immunoglobulin genes are expressed in the myeloma S107. Cell. 1981 Oct;26(1 Pt 1):57–66. doi: 10.1016/0092-8674(81)90033-7. [DOI] [PubMed] [Google Scholar]
  14. Mage R. G., Dray S., Gilman-Sachs A., Hamers-Casterman C., Hamers R., Hanly W. C., Kindt T. J., Knight K. L., Mandy W. J., Naessens J. Rabbit heavy chain haplotypes--allotypic determinants expressed by VH-CH recombinants. Immunogenetics. 1982 Mar;15(3):287–297. doi: 10.1007/BF00364337. [DOI] [PubMed] [Google Scholar]
  15. McCartney-Francis N., Mandy W. J. Control of latent allotype expression by rabbit splenocytes. J Immunol. 1981 Jul;127(1):352–357. [PubMed] [Google Scholar]
  16. Otsuka A. Recovery of DNA fragments inserted by the "tailing" method: regeneration of PstI restriction sites. Gene. 1981 May;13(4):339–346. doi: 10.1016/0378-1119(81)90013-5. [DOI] [PubMed] [Google Scholar]
  17. Roux K. H. Rabbit latent allotypes: current status and future directions. Surv Immunol Res. 1983;2(4):342–350. doi: 10.1007/BF02918451. [DOI] [PubMed] [Google Scholar]
  18. Sheppard H. W., Gutman G. A. Allelic forms of rat kappa chain genes: evidence for strong selection at the level of nucleotide sequence. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7064–7068. doi: 10.1073/pnas.78.11.7064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thayer R. E. An improved method for detecting foreign DNA in plasmids of Escherichia coli. Anal Biochem. 1979 Sep 15;98(1):60–63. doi: 10.1016/0003-2697(79)90705-x. [DOI] [PubMed] [Google Scholar]
  20. Wabl M., Steinberg C. A theory of allelic and isotypic exclusion for immunoglobulin genes. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6976–6978. doi: 10.1073/pnas.79.22.6976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weiss S., Garcia I., Jaton J. C., Kelus A. S. Allotypes in Basilea rabbits. Eur J Immunol. 1982 Feb;12(2):170–173. doi: 10.1002/eji.1830120213. [DOI] [PubMed] [Google Scholar]
  22. Wu G. E., Hozumi N., Murialdo H. Secretion of a lambda 2 immunoglobulin chain is prevented by a single amino acid substitution in its variable region. Cell. 1983 May;33(1):77–83. doi: 10.1016/0092-8674(83)90336-7. [DOI] [PubMed] [Google Scholar]

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