Abstract
DNA sequences homologous to adult alpha-globin genes are dispersed in the mouse. Two functional genes are tightly linked on chromosome 11. Pseudogenes have been assigned to chromosomes 15 and 17 by analysis of interspecies somatic cell hybrids. We have now further characterized the second of these pseudogenes, Hba-a4. The gene is highly polymorphic, with three forms occurring in a panel of 15 inbred strains and a fourth occurring in an inbred strain derived from M. m. molossinus. Analysis of Hba-a4 alleles in CXB, BXH, and AKXL recombinant inbred strains placed Hba-a4 6.60 +/- 3.14 cM centromeric to H-2. Analysis of congenic mouse strains confirmed the linkage and the gene order. Hba-a4 is the first mammalian dispersed pseudogene to be localized in a linkage map, and should provide a useful marker for the region of chromosome 17 proximal to H-2.
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- Childs G., Maxson R., Cohn R. H., Kedes L. Orphons: dispersed genetic elements derived from tandem repetitive genes of eucaryotes. Cell. 1981 Mar;23(3):651–663. doi: 10.1016/0092-8674(81)90428-1. [DOI] [PubMed] [Google Scholar]
- D'Eustachio P., Bothwell A. L., Takaro T. K., Baltimore D., Ruddle F. H. Chromosomal location of structural genes encoding murine immunoglobulin lambda light chains. Genetics of murine lambda light chains. J Exp Med. 1981 Apr 1;153(4):793–800. doi: 10.1084/jem.153.4.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
- D'Eustachio P., Ruddle F. H. Somatic cell genetics and gene families. Science. 1983 May 27;220(4600):919–924. doi: 10.1126/science.6573776. [DOI] [PubMed] [Google Scholar]
- Klein J., Figueroa F., Klein D. H-2 haplotypes, genes, and antigens: second listing. I. Non-H-2 loci on chromosome 17. Immunogenetics. 1982;16(4):285–317. doi: 10.1007/BF00372302. [DOI] [PubMed] [Google Scholar]
- Leder A., Swan D., Ruddle F., D'Eustachio P., Leder P. Dispersion of alpha-like globin genes of the mouse to three different chromosomes. Nature. 1981 Sep 17;293(5829):196–200. doi: 10.1038/293196a0. [DOI] [PubMed] [Google Scholar]
- Leder P., Hansen J. N., Konkel D., Leder A., Nishioka Y., Talkington C. Mouse globin system: a functional and evolutionary analysis. Science. 1980 Sep 19;209(4463):1336–1342. doi: 10.1126/science.7414319. [DOI] [PubMed] [Google Scholar]
- Marcu K. B., Banerji J., Penncavage N. A., Lang R., Arnheim N. 5' flanking region of immunoglobulin heavy chain constant region genes displays length heterogeneity in germlines of inbred mouse strains. Cell. 1980 Nov;22(1 Pt 1):187–196. doi: 10.1016/0092-8674(80)90167-1. [DOI] [PubMed] [Google Scholar]
- Michaelson J. Genetics of beta-2 microglobulin in the mouse. Immunogenetics. 1983;17(3):219–260. doi: 10.1007/BF00364409. [DOI] [PubMed] [Google Scholar]
- Popp R. A., Lalley P. A., Whitney J. B., 3rd, Anderson W. F. Mouse alpha-globin genes and alpha-globin-like pseudogenes are not syntenic. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6362–6366. doi: 10.1073/pnas.78.10.6362. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Rinchik E. M., Amos D. B. Genetic analysis of the T-H-2 region in non-t chromosomes. I. Two new congenic strains isolate Qglo-1 from H-2. Immunogenetics. 1983;17(5):445–455. doi: 10.1007/BF00696868. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Szymura J. M., Taylor B. A., Klein J. Upg-2: a urinary pepsinogen variant located on chromosome 1 of the mouse. Biochem Genet. 1982 Dec;20(11-12):1211–1219. doi: 10.1007/BF00498944. [DOI] [PubMed] [Google Scholar]
- Vanin E. F., Goldberg G. I., Tucker P. W., Smithies O. A mouse alpha-globin-related pseudogene lacking intervening sequences. Nature. 1980 Jul 17;286(5770):222–226. doi: 10.1038/286222a0. [DOI] [PubMed] [Google Scholar]