Abstract
Schistosoma mansoni schistosomula recovered from the lungs of mice have previously been shown to express host-derived class I and class II major histocompatibility complex (MHC) antigens. To investigate the tissue origin of parasite-acquired MHC products, lung-stage schistosomula were obtained from a series of parent leads to F1 and F1 leads to parent bone marrow chimeras and the parasites typed by immunofluorescence for the presence of haplotype-specific K region and I region MHC determinants. The results of these experiments indicated that, despite their intravascular residence in the host, schistosomula derive all of their class I antigen from a nonhemapoietic tissue source. In contrast, the class II antigens expressed on the surface of schistosomula were found to originate from bone marrow-derived donor cells. These results support the hypothesis that MHC product acquisition by schistosomes involves selective and specific interactions with host tissue and, in the case of class I antigens, suggest that the endothelium may be a major site of host molecule uptake for the parasite.
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Selected References
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- Butterworth A. E., Vadas M. A., Martz E., Sher A. Cytolytic T lymphocytes recognize alloantigens on schistosomula of Schistosoma mansoni, but fail to induce damage. J Immunol. 1979 Apr;122(4):1314–1321. [PubMed] [Google Scholar]
- Emerson S. G., Cone R. E. I-Kk and H-2Kk antigens are shed as supramolecular particles in association with membrane lipids. J Immunol. 1981 Aug;127(2):482–486. [PubMed] [Google Scholar]
- Gitter B. D., Damian R. T. Murine alloantigen acquisition by schistosomula of Schistosoma mansoni: further evidence for the presence of K, D, and I region gene products on the tegumental surface. Parasite Immunol. 1982 Nov;4(6):383–393. doi: 10.1111/j.1365-3024.1982.tb00450.x. [DOI] [PubMed] [Google Scholar]
- Gitter B. D., McCormick S. L., Damian R. T. Murine alloantigen acquisition by Schistosoma mansoni: presence of H-2K determinants on adult worms and failure of allogeneic lymphocytes to recognize acquired MHC gene products on schistosomula. J Parasitol. 1982 Aug;68(4):513–518. [PubMed] [Google Scholar]
- Kvist S., Peterson P. A. Isolation and partial characterization of a beta2-microglobulin-containing, H-2 antigen-like murine serum protein. Biochemistry. 1978 Oct 31;17(22):4794–4801. doi: 10.1021/bi00615a029. [DOI] [PubMed] [Google Scholar]
- Lazdins J. K., Stein M. J., David J. R., Sher A. Schistosoma mansoni: rapid isolation and purification of schistosomula of different developmental stages by centrifugation on discontinuous density gradients of Percoll. Exp Parasitol. 1982 Feb;53(1):39–44. doi: 10.1016/0014-4894(82)90090-x. [DOI] [PubMed] [Google Scholar]
- McLaren D. J., Terry R. J. The protective role of acquired host antigens during schistosome maturation. Parasite Immunol. 1982 Mar;4(2):129–148. doi: 10.1111/j.1365-3024.1982.tb00426.x. [DOI] [PubMed] [Google Scholar]
- Pober J. S., Gimbrone M. A., Jr, Cotran R. S., Reiss C. S., Burakoff S. J., Fiers W., Ault K. A. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon. J Exp Med. 1983 Apr 1;157(4):1339–1353. doi: 10.1084/jem.157.4.1339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Samuelson J. C., Sher A., Caulfield J. P. Newly transformed schistosomula spontaneously lose surface antigens and C3 acceptor sites during culture. J Immunol. 1980 Apr;124(4):2055–2057. [PubMed] [Google Scholar]
- Sher A., Hall B. F., Vadas M. A. Acquisition of murine major histocompatibility complex gene products by schistosomula of Schistosoma mansoni. J Exp Med. 1978 Jul 1;148(1):46–57. doi: 10.1084/jem.148.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simpson A. J., Singer D., McCutchan T. F., Sacks D. L., Sher A. Evidence that schistosome MHC antigens are not synthesized by the parasite but are acquired from the host as intact glycoproteins. J Immunol. 1983 Aug;131(2):962–965. [PubMed] [Google Scholar]
- Singer A., Hathcock K. S., Hodes R. J. Cellular and genetic control of antibody responses. V. Helper T-cell recognition of H-2 determinants on accessory cells but not B cells. J Exp Med. 1979 May 1;149(5):1208–1226. doi: 10.1084/jem.149.5.1208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smithers S. R., Terry R. J., Hockley D. J. Host antigens in schistosomiasis. Proc R Soc Lond B Biol Sci. 1969 Feb 25;171(1025):483–494. doi: 10.1098/rspb.1969.0007. [DOI] [PubMed] [Google Scholar]
- Vadas M. A., Butterworth A. E., Burakoff S., Sher A. Major histocompatibility complex products restrict the adherence of cytolytic T lymphocytes to minor histocompatibility antigens or to trinitrophenyl determinants on schistosomula of Schistosoma mansoni. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1982–1985. doi: 10.1073/pnas.76.4.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]