Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1984 Apr 1;159(4):1105–1116. doi: 10.1084/jem.159.4.1105

Rat lymphoid cell lines with human T cell leukemia virus production. I. Biological and serological characterization

PMCID: PMC2187267  PMID: 6323614

Abstract

Cocultivation of spleen cells, lymph node cells, and thymocytes of female Wistar-King-Aptekman rats with short-term cultured male adult T cell leukemia (ATL) cells in the presence of 5-bromo-2'-deoxyuridine (BrdUrd) resulted in the establishment of rat lymphoid cell lines, TARS- 1, TARL-2, and TART-1. Cytogenetic analysis of the three cell lines showed a female rat karyotype with 42 chromosomes. The surface phenotypes of TARS-1 and TART-1 were those of rat T cells. TARL-2 was only positive for rat Ia and leukocyte common antigens. The cell lines continuously produced a type C retrovirus, human T cell leukemia virus (HTLV) and expressed ATL-associated antigens. TARS-1 and TART-1, but not TARL-2 were transplantable into newborn syngeneic rats and nude mice. These results strongly indicate that HTLV not only immortalizes, but also transforms rat T cells in vitro. Adult rats immunized with either TARS-1 or TARL-2 produced antibodies specific for HTLV. The biochemical analysis of the antigens that reacted with rat sera revealed that they are the two HTLV-specific polypeptides, p24 and p28.

Full Text

The Full Text of this article is available as a PDF (1.8 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Haynes B. F., Miller S. E., Palker T. J., Moore J. O., Dunn P. H., Bolognesi D. P., Metzgar R. S. Identification of human T cell leukemia virus in a Japanese patient with adult T cell leukemia and cutaneous lymphomatous vasculitis. Proc Natl Acad Sci U S A. 1983 Apr;80(7):2054–2058. doi: 10.1073/pnas.80.7.2054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hinuma Y., Nagata K., Hanaoka M., Nakai M., Matsumoto T., Kinoshita K. I., Shirakawa S., Miyoshi I. Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476–6480. doi: 10.1073/pnas.78.10.6476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ikeda H., Pincus T., Yoshiki T., Strand M., August J. T., Boyse E. A., Mellors R. C. Biological expression of antigenic determinants of murine leukemia virus proteins gp69-71 and p30. J Virol. 1974 Nov;14(5):1274–1280. doi: 10.1128/jvi.14.5.1274-1280.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ishii Y., Matsuura A., Yuasa H., Narita H., Takami T., Kikuchi K. Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies. Immunology. 1983 Apr;48(4):743–754. [PMC free article] [PubMed] [Google Scholar]
  5. Kalyanaraman V. S., Sarngadharan M. G., Nakao Y., Ito Y., Aoki T., Gallo R. C. Natural antibodies to the structural core protein (p24) of the human T-cell leukemia (lymphoma) retrovirus found in sera of leukemia patients in Japan. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1653–1657. doi: 10.1073/pnas.79.5.1653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Miyoshi I., Kubonishi I., Sumida M., Hiraki S., Tsubota T., Kimura I., Miyamoto K., Sato J. A novel T-cell line derived from adult T-cell leukemia. Gan. 1980 Feb;71(1):155–156. [PubMed] [Google Scholar]
  7. Miyoshi I., Kubonishi I., Yoshimoto S., Akagi T., Ohtsuki Y., Shiraishi Y., Nagata K., Hinuma Y. Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells. Nature. 1981 Dec 24;294(5843):770–771. doi: 10.1038/294770a0. [DOI] [PubMed] [Google Scholar]
  8. Miyoshi I., Taguchi H., Fujishita M., Yoshimoto S., Kubonishi I., Ohtsuki Y., Shiraishi Y., Akagi T. Transformation of monkey lymphocytes with adult T-cell leukaemia virus. Lancet. 1982 May 1;1(8279):1016–1016. doi: 10.1016/s0140-6736(82)92012-8. [DOI] [PubMed] [Google Scholar]
  9. Miyoshi I., Yoshimoto S., Kubonishi I., Taguchi H., Shiraishi Y., Ohtsuki Y., Akagi T. Transformation of normal human cord lymphocytes by co-cultivation with a lethally irradiated human T-cell line carrying type C virus particles. Gan. 1981 Dec;72(6):997–998. [PubMed] [Google Scholar]
  10. Miyoshi I., Yoshimoto S., Ohtsuki Y., Fujishita M., Akagi T., Yoshiki T., Koike T. Adult T-cell leukaemia antigen in renal glomerulus. Lancet. 1983 Apr 2;1(8327):768–769. doi: 10.1016/s0140-6736(83)92060-3. [DOI] [PubMed] [Google Scholar]
  11. Miyoshi I., Yoshimoto S., Taguchi H., Kubonishi I., Fujishita M., Ohtsuki Y., Shiraishi Y., Akagi T. Transformation of rabbit lymphocytes with T-cell leukemia virus. Gan. 1983 Feb;74(1):1–4. [PubMed] [Google Scholar]
  12. Poiesz B. J., Ruscetti F. W., Gazdar A. F., Bunn P. A., Minna J. D., Gallo R. C. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415–7419. doi: 10.1073/pnas.77.12.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Poiesz B. J., Ruscetti F. W., Reitz M. S., Kalyanaraman V. S., Gallo R. C. Isolation of a new type C retrovirus (HTLV) in primary uncultured cells of a patient with Sézary T-cell leukaemia. Nature. 1981 Nov 19;294(5838):268–271. doi: 10.1038/294268a0. [DOI] [PubMed] [Google Scholar]
  14. Popovic M., Sarin P. S., Robert-Gurroff M., Kalyanaraman V. S., Mann D., Minowada J., Gallo R. C. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science. 1983 Feb 18;219(4586):856–859. doi: 10.1126/science.6600519. [DOI] [PubMed] [Google Scholar]
  15. Reitz M. S., Jr, Poiesz B. J., Ruscetti F. W., Gallo R. C. Characterization and distribution of nucleic acid sequences of a novel type C retrovirus isolated from neoplastic human T lymphocytes. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1887–1891. doi: 10.1073/pnas.78.3.1887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Robert-Guroff M., Fahey K. A., Maeda M., Nakao Y., Ito Y., Gallo R. C. Identification of HTLV p19 specific natural human antibodies by competition with monoclonal antibody. Virology. 1982 Oct 30;122(2):297–305. doi: 10.1016/0042-6822(82)90229-x. [DOI] [PubMed] [Google Scholar]
  17. Robert-Guroff M., Kalyanaraman V. S., Blattner W. A., Popovic M., Sarngadharan M. G., Maeda M., Blayney D., Catovsky D., Bunn P. A., Shibata A. Evidence for human T cell lymphoma-leukemia virus infection of family members of human T cell lymphoma-leukemia virus positive T cell leukemia-lymphoma patients. J Exp Med. 1983 Jan 1;157(1):248–258. doi: 10.1084/jem.157.1.248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sarin P. S., Aoki T., Shibata A., Ohnishi Y., Aoyagi Y., Miyakoshi H., Emura I., Kalyanaraman V. S., Robert-Guroff M., Popovic M. High incidence of human type-C retrovirus (HTLV) in family members of a HTLV-positive Japanese T-cell leukemia patient. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2370–2374. doi: 10.1073/pnas.80.8.2370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Seiki M., Hattori S., Hirayama Y., Yoshida M. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618–3622. doi: 10.1073/pnas.80.12.3618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shirai T., Yoshiki T., Mellors R. C. Thymus dependence of cells in peripheral lymphoid tissues and in the circulation sensitive to natural thymocytotoxic autoantibody in NZB mice. J Immunol. 1972 Jul;109(1):32–37. [PubMed] [Google Scholar]
  21. Tanaka Y., Koyanagi Y., Chosa T., Yamamoto N., Hinuma Y. Monoclonal antibody reactive with both p28 and p19 of adult T-cell leukemia virus-specific polypeptides. Gan. 1983 Jun;74(3):327–330. [PubMed] [Google Scholar]
  22. Uchiyama T., Yodoi J., Sagawa K., Takatsuki K., Uchino H. Adult T-cell leukemia: clinical and hematologic features of 16 cases. Blood. 1977 Sep;50(3):481–492. [PubMed] [Google Scholar]
  23. Yamamoto N., Hinuma Y. Antigens in an adult T-cell leukemia virus-producer cell line: reactivity with human serum antibodies. Int J Cancer. 1982 Sep 15;30(3):289–293. doi: 10.1002/ijc.2910300306. [DOI] [PubMed] [Google Scholar]
  24. Yamamoto N., Okada M., Koyanagi Y., Kannagi M., Hinuma Y. Transformation of human leukocytes by cocultivation with an adult T cell leukemia virus producer cell line. Science. 1982 Aug 20;217(4561):737–739. doi: 10.1126/science.6980467. [DOI] [PubMed] [Google Scholar]
  25. Yoshida M., Miyoshi I., Hinuma Y. Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2031–2035. doi: 10.1073/pnas.79.6.2031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yoshiki T., Mellors R. C., Hardy W. D., Jr, Fleissner E. Common cell surface antigen associated with mammalian C-type RNA viruses. Cell membrane-bound gs antigen. J Exp Med. 1974 Apr 1;139(4):925–942. doi: 10.1084/jem.139.4.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yoshiki T., Mellors R. C., Strand M., August J. T. The viral envelope glycoprotein of murine leukemia virus and the pathogenesis of immune complex glomerulonephritis of New Zealand mice. J Exp Med. 1974 Oct 1;140(4):1011–1027. doi: 10.1084/jem.140.4.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES