Abstract
Previous reports suggest that large numbers of immunoglobulin-secreting cells appear in tissues of NZB strain mice from the time of birth. In this study, we investigated the development of B lineage cells during embryonic life and found that they were present 2-3 d earlier and in higher numbers in NZB embryos than several other strains of mice. That is, liver cell suspensions from NZB embryos contained larger numbers of surface Ig (sIg)- cells that could form B cell colonies in mitogen- dependent semisolid agar culture. Sephadex G-10-adherent cell depletion diminished numbers of colonies and this was partially restored by addition of humoral factors. The latter were partially purified from serum of very young NZB mice. These findings document that abnormal changes take place in B lineage cells and possibly also in cells that regulate their maturation in NZB strain mice at a very early stage of development.
Full Text
The Full Text of this article is available as a PDF (452.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DeHeer D. H., Edgington T. S. Evidence for a B lymphocyte defect underlying the anti-X anti-erythrocyte autoantibody response of NZB mice. J Immunol. 1977 May;118(5):1858–1863. [PubMed] [Google Scholar]
- Johnson G. R., Metcalf D., Wilson J. W. Development of B-lymphocyte colony-forming cells in foetal mouse tissues. Immunology. 1976 Jun;30(6):907–914. [PMC free article] [PubMed] [Google Scholar]
- Jyonouchi H., Kincade P. W. Changes in B lineage cell population in liver and spleen of normal neonatal mice. J Immunol. 1983 Apr;130(4):1616–1621. [PubMed] [Google Scholar]
- Jyonouchi H., Kincade P. W., Good R. A., Gershwin M. E. B lymphocyte lineage cells in newborn and very young NZB mice: evidence for regulatory disorders affecting B cell formation. J Immunol. 1983 Nov;131(5):2219–2225. [PubMed] [Google Scholar]
- Jyonouchi H., Kincade P. W., Landreth K. S., Lee G., Good R. A., Gershwin M. E. Age-dependent deficiency of B lymphocyte lineage precursors in NZB mice. J Exp Med. 1982 Jun 1;155(6):1665–1678. doi: 10.1084/jem.155.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kincade P. W. Formation of B lymphocytes in fetal and adult life. Adv Immunol. 1981;31:177–245. doi: 10.1016/s0065-2776(08)60921-9. [DOI] [PubMed] [Google Scholar]
- Kincade P. W., Lee G., Paige C. J., Scheid M. P. Cellular interactions affecting the maturation of murine B lymphocyte precursors in vitro. J Immunol. 1981 Jul;127(1):255–260. [PubMed] [Google Scholar]
- Kincade P. W., Lee G., Scheid M. P., Blum M. D. Characterization of murine colony-forming B cells. II. Limits to in vitro maturation, Lyb-2 expression, resolution of IgD+ subsets, and further population analysis. J Immunol. 1980 Feb;124(2):947–953. [PubMed] [Google Scholar]
- Landreth K. S., Kincade P. W., Lee G., Medlock E. S. Phenotypic and functional characterization of murine B lymphocyte precursors isolated from fetal and adult tissues. J Immunol. 1983 Aug;131(2):572–580. [PubMed] [Google Scholar]
- Manny N., Datta S. K., Schwartz R. S. Synthesis of IgM by cells of NZB and SWR mice and their crosses. J Immunol. 1979 Apr;122(4):1220–1227. [PubMed] [Google Scholar]
- Milich D. R., Gershwin M. E. The pathogenesis of autoimmunity in New Zealand mice. Semin Arthritis Rheum. 1980 Nov;10(2):111–147. doi: 10.1016/0049-0172(80)90004-9. [DOI] [PubMed] [Google Scholar]
- Mosier D. E., Cohen P. L. Ontogeny of mouse T-lymphocyte function. Fed Proc. 1975 Feb;34(2):137–140. [PubMed] [Google Scholar]
- Moutsopoulos H. M., Boehm-Truitt M., Kassan S. S., Chused T. M. Demonstration of activation of B lymphocytes in New Zealand black mice at birth by an immunoradiometric assay for murine IgM. J Immunol. 1977 Nov;119(5):1639–1644. [PubMed] [Google Scholar]
- Ohsugi Y., Gershwin M. E. Studies of congenitally immunologic mutant New Zealand mice. III. Growth of B lymphocyte clones in congenitally athymic (nude) and hereditarily asplenic (Dh/+) NZB mice: a primary B cell defect. J Immunol. 1979 Sep;123(3):1260–1265. [PubMed] [Google Scholar]
- Playfair J. H. Strain differences in the immune response of mice. I. The neonatal response to sheep red cells. Immunology. 1968 Jul;15(1):35–50. [PMC free article] [PubMed] [Google Scholar]
- Stanley E. R. Colony-stimulating factor (CSF) radioimmunoassay: detection of a CSF subclass stimulating macrophage production. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2969–2973. doi: 10.1073/pnas.76.6.2969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinberg A. D., Huston D. P., Taurog J. D., Cowdery J. S., Ravecheé E. S. The cellular and genetic basis of murine lupus. Immunol Rev. 1981;55:121–154. doi: 10.1111/j.1600-065x.1981.tb00341.x. [DOI] [PubMed] [Google Scholar]
- Taurog J. D., Raveche E. S., Smathers P. A., Glimcher L. H., Huston D. P., Hansen C. T., Steinberg A. D. T cell abnormalities in NZB mice occur independently of autoantibody production. J Exp Med. 1981 Feb 1;153(2):221–234. doi: 10.1084/jem.153.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]