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. 1984 May 1;159(5):1455–1463. doi: 10.1084/jem.159.5.1455

Heterogeneity among human collagenases demonstrated by monoclonal antibody that selectively recognizes and inhibits human neutrophil collagenase

PMCID: PMC2187304  PMID: 6325574

Abstract

The heterogeneity of human collagenases has been examined using a monoclonal antibody to neutrophil collagenase. This antibody inhibited collagenase activity and, when covalently coupled to Sepharose, bound both latent and active enzyme. Although human neutrophil collagenase was inhibited by the antibody, the activity of human skin and rheumatoid synovial collagenase was not significantly diminished in the presence of the antibody. Competitive inhibition studies also differentiated between these collagenases. Only human neutrophil collagenase effectively blocked the antibody in a competitive enzyme- linked immunosorbent assay while skin and rheumatoid synovial collagenase again failed to interact with the antibody. The unequivocal recognition of neutrophil collagenase as an immunologically distinct entity from other collagenases supports the hypothesis that neutrophil collagenase is a separate gene product from fibroblast or synovial collagenase.

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Selected References

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  1. Bauer E. A., Eisen A. Z., Jeffrey J. J. Radioimmunoassay of human collagenase. Specificity of the assay and quantitative determination of in vivo and in vitro human skin collagenase. J Biol Chem. 1972 Oct 25;247(20):6679–6685. [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. Cawston T. E., Barrett A. J. A rapid and reproducible assay for collagenase using [1-14C]acetylated collagen. Anal Biochem. 1979 Nov 1;99(2):340–345. doi: 10.1016/s0003-2697(79)80017-2. [DOI] [PubMed] [Google Scholar]
  4. Christner P., Damato D., Reinhart M., Abrams W. Purification of human neutrophil collagenase and production of a monospecific antiserum. Biochemistry. 1982 Nov 9;21(23):6005–6011. doi: 10.1021/bi00266a043. [DOI] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  6. GLIMCHER M. J., FRANCOIS C. J., RICHARDS L., KRANE S. M. THE PRESENCE OF ORGANIC PHOSPHORUS IN COLLAGENS AND GELATINS. Biochim Biophys Acta. 1964 Dec 9;93:585–602. doi: 10.1016/0304-4165(64)90342-3. [DOI] [PubMed] [Google Scholar]
  7. Gadek J. E., Kelman J. A., Fells G., Weinberger S. E., Horwitz A. L., Reynolds H. Y., Fulmer J. D., Crystal R. G. Collagenase in the lower respiratory tract of patients with idiopathic pulmonary fibrosis. N Engl J Med. 1979 Oct 4;301(14):737–742. doi: 10.1056/NEJM197910043011401. [DOI] [PubMed] [Google Scholar]
  8. Harris E. D., Jr, DiBona D. R., Krane S. M. Collagenases in human synovial fluid. J Clin Invest. 1969 Nov;48(11):2104–2113. doi: 10.1172/JCI106177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harris E. D., Jr, Faulkner C. S., 2nd, Brown F. E. Collagenolytic systems in rheumatoid arthritis. Clin Orthop Relat Res. 1975 Jul-Aug;(110):303–316. doi: 10.1097/00003086-197507000-00041. [DOI] [PubMed] [Google Scholar]
  10. Hasty D. L., Beachey E. H., Simpson W. A., Dale J. B. Hybridoma antibodies against protective and nonprotective antigenic determinants of a structurally defined polypeptide fragment of streptococcal M protein. J Exp Med. 1982 Apr 1;155(4):1010–1018. doi: 10.1084/jem.155.4.1010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Horwitz A. L., Hance A. J., Crystal R. G. Granulocyte collagenase: selective digestion of type I relative to type III collagen. Proc Natl Acad Sci U S A. 1977 Mar;74(3):897–901. doi: 10.1073/pnas.74.3.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lazarus G. S., Brown R. S., Daniels J. R., Fullmer H. M. Human granulocyte collagenase. Science. 1968 Mar 29;159(3822):1483–1485. doi: 10.1126/science.159.3822.1483. [DOI] [PubMed] [Google Scholar]
  14. Mainardi C. L., Seyer J. M., Kang A. H. Type-specific collagenolysis: a type V collagen-degrading enzyme from macrophages. Biochem Biophys Res Commun. 1980 Dec 16;97(3):1108–1115. doi: 10.1016/0006-291x(80)91490-4. [DOI] [PubMed] [Google Scholar]
  15. Murphy G., Bretz U., Baggiolini M., Reynolds J. J. The latent collagenase and gelatinase of human polymorphonuclear neutrophil leucocytes. Biochem J. 1980 Nov 15;192(2):517–525. doi: 10.1042/bj1920517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murphy G., Reynolds J. J., Bretz U., Baggiolini M. Partial purification of collagenase and gelatinase from human polymorphonuclear leucocytes. Analysis of their actions on soluble and insoluble collagens. Biochem J. 1982 Apr 1;203(1):209–221. doi: 10.1042/bj2030209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nagai Y., Lapiere C. M., Gross J. Tadpole collagenase. Preparation and purification. Biochemistry. 1966 Oct;5(10):3123–3130. doi: 10.1021/bi00874a007. [DOI] [PubMed] [Google Scholar]
  18. Ohlsson K., Olsson I. The neutral proteases of human granulocytes. Isolation and partial characterization of two granulocyte collagenases. Eur J Biochem. 1973 Jul 16;36(2):473–481. doi: 10.1111/j.1432-1033.1973.tb02932.x. [DOI] [PubMed] [Google Scholar]
  19. Reed C. F. Incorporation of orthophosphate-32P into erythrocyte phospholipids in normal subjects and in patients with hereditary spherocytosis. J Clin Invest. 1968 Dec;47(12):2630–2638. doi: 10.1172/JCI105946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rennard S. I., Berg R., Martin G. R., Foidart J. M., Robey P. G. Enzyme-linked immunoassay (ELISA) for connective tissue components. Anal Biochem. 1980 May 1;104(1):205–214. doi: 10.1016/0003-2697(80)90300-0. [DOI] [PubMed] [Google Scholar]
  21. Sellers A., Cartwright E., Murphy G., Reynolds J. J. Evidence that latent collagenases are enzyme-inhibitor complexes. Biochem J. 1977 May 1;163(2):303–307. doi: 10.1042/bj1630303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stricklin G. P., Bauer E. A., Jeffrey J. J., Eisen A. Z. Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ cultures. Biochemistry. 1977 Apr 19;16(8):1607–1615. doi: 10.1021/bi00627a013. [DOI] [PubMed] [Google Scholar]
  23. Vater C. A., Mainardi C. L., Harris E. D., Jr Activation in vitro of rheumatoid synovial collagenase from cell cultures. J Clin Invest. 1978 Nov;62(5):987–992. doi: 10.1172/JCI109228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Werb Z., Burleigh M. C., Barrett A. J., Starkey P. M. The interaction of alpha2-macroglobulin with proteinases. Binding and inhibition of mammalian collagenases and other metal proteinases. Biochem J. 1974 May;139(2):359–368. doi: 10.1042/bj1390359. [DOI] [PMC free article] [PubMed] [Google Scholar]

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