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. 1983 Aug 1;158(2):303–316. doi: 10.1084/jem.158.2.303

Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing

PMCID: PMC2187345  PMID: 6193218

Abstract

We examined the ability of a set of cloned chicken ovalbumin (cOVA)- specific, Id-restricted, T cell hybridomas to produce interleukin-2 in response to cOVA presented by the Ia+ B cell lymphoma line, A20-2J. Although viable A20-2J cells presented native, denatured, and fragmented cOVA more or less equally well, A20-2J cells that were glutaraldehyde-fixed could present only enzymatically or chemically fragmented cOVA. These results suggest that antigen fragmentation may be both necessary and sufficient to define accessory cell processing of soluble antigens so that they may be recognized in association with I- region molecules by T cells.

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Selected References

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