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. 1983 Oct 1;158(4):1344–1349. doi: 10.1084/jem.158.4.1344

Human Ia beta chains and the invariant chain share a common antigenic determinant

PMCID: PMC2187380  PMID: 6194245

Abstract

We have presented evidence that a mouse monoclonal antibody 1,227, which was previously shown to recognize a determinant on the light chains beta 1 and beta 2 of human Ia antigens, also recognizes a determinant on the invariant chain (1) associated with these molecules. Ia-specific proteins were resolved by two-dimensional (2D) PAGE and electrophoretically transferred onto nitrocellulose filters. The presence of a determinant shared between beta 1, beta 2 and I was established using "Western blotting" technique. In addition, we demonstrated that 1,227 can immunoprecipitate isolated beta 1, beta 2, and the invariant chain proteins following their separate elution from SDS-PAGE.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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