Abstract
It is known that polycations bind to and neutralize glomerular polyanions. Here we examine the effect of the polycation polyethyleneimine (PEI) on glomerular deposition of preformed immune complexes. Bovine serum albumin (BSA)-anti-BSA immune complexes made in 40 times antigen excess were administered following intravenous injection of PEI. Glomerular localization of immune deposits was assessed by quantitative immunofluorescence and electron microscopy and compared to controls receiving diluent without PEI followed by the same dose in immune complexes. In rats receiving PEI, deposits were localized within the glomerular basement membrane (GBM) of all peripheral capillary walls and in the mesangium. In controls, deposits localized exclusively within the mesangium in smaller amounts than after PEI. Thus, neutralization of glomerular polyanion by a circulating polycation enhances the deposition and alters the distribution of immune complexes in glomeruli.
Full Text
The Full Text of this article is available as a PDF (849.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnes J. L., Levine S. P., Venkatachalam M. A. Binding of platelet factor four (PF 4) to glomerular polyanion. Kidney Int. 1984 May;25(5):759–765. doi: 10.1038/ki.1984.87. [DOI] [PubMed] [Google Scholar]
- Barnes J. L., Radnik R. A., Gilchrist E. P., Venkatachalam M. A. Size and charge selective permeability defects induced in glomerular basement membrane by a polycation. Kidney Int. 1984 Jan;25(1):11–19. doi: 10.1038/ki.1984.2. [DOI] [PubMed] [Google Scholar]
- Border W. A., Ward H. J., Kamil E. S., Cohen A. H. Induction of membranous nephropathy in rabbits by administration of an exogenous cationic antigen. J Clin Invest. 1982 Feb;69(2):451–461. doi: 10.1172/JCI110469. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Camussi G., Tetta C., Segoloni G., Coda R., Vercellone A. Localization of neutrophil cationic proteins and loss of anionic charges in glomeruli of patients with systemic lupus erythematosus glomerulonephritis. Clin Immunol Immunopathol. 1982 Sep;24(3):299–314. doi: 10.1016/0090-1229(82)90001-0. [DOI] [PubMed] [Google Scholar]
- Courtoy P. J., Picton D. H., Farquhar M. G. Resolution and limitations of the immunoperoxidase procedure in the localization of extracellular matrix antigens. J Histochem Cytochem. 1983 Jul;31(7):945–951. doi: 10.1177/31.7.6304184. [DOI] [PubMed] [Google Scholar]
- Gallo G. R., Caulin-Glaser T., Lamm M. E. Charge of circulating immune complexes as a factor in glomerular basement membrane localization in mice. J Clin Invest. 1981 May;67(5):1305–1313. doi: 10.1172/JCI110159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Germuth F. G., Jr, Rodriguez E., Lorelle C. A., Trump E. I., Milano L., Wise O. Passive immune complex glomerulonephritis in mice: models for various lesions found in human disease. I. High avidity complexes and mesangiopathic glomerulonephritis. Lab Invest. 1979 Oct;41(4):360–365. [PubMed] [Google Scholar]
- Hunsicker L. G., Shearer T. P., Shaffer S. J. Acute reversible proteinuria induced by infusion of the polycation hexadimethrine. Kidney Int. 1981 Jul;20(1):7–17. doi: 10.1038/ki.1981.98. [DOI] [PubMed] [Google Scholar]
- Kelley V. E., Cavallo T. Glomerular permeability: transfer of native ferritin in glomeruli with decreased anionic sites. Lab Invest. 1978 Dec;39(6):547–553. [PubMed] [Google Scholar]
- Melnick G. F., Ladoulis C. T., Cavallo T. Decreased anionic groups and increased permeability precedes deposition of immune complexes in the glomerular capillary wall. Am J Pathol. 1981 Nov;105(2):114–120. [PMC free article] [PubMed] [Google Scholar]
- Oite T., Batsford S. R., Mihatsch M. J., Takamiya H., Vogt A. Quantitative studies of in situ immune complex glomerulonephritis in the rat induced by planted, cationized antigen. J Exp Med. 1982 Feb 1;155(2):460–474. doi: 10.1084/jem.155.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rennke H. G., Cotran R. S., Venkatachalam M. A. Role of molecular charge in glomerular permeability. Tracer studies with cationized ferritins. J Cell Biol. 1975 Dec;67(3):638–646. doi: 10.1083/jcb.67.3.638. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schurer J. W., Hoedemaeker J., Molenaar I. Polyethyleneimine as tracer particle for (immuno) electron microscopy. J Histochem Cytochem. 1977 May;25(5):384–387. doi: 10.1177/25.5.325123. [DOI] [PubMed] [Google Scholar]
- Vehaskari V. M., Root E. R., Germuth F. G., Jr, Robson A. M. Glomerular charge and urinary protein excretion: effects of systemic and intrarenal polycation infusion in the rat. Kidney Int. 1982 Aug;22(2):127–135. doi: 10.1038/ki.1982.144. [DOI] [PubMed] [Google Scholar]
- Venkatachalam M. A., Rennke H. G. The structural and molecular basis of glomerular filtration. Circ Res. 1978 Sep;43(3):337–347. doi: 10.1161/01.res.43.3.337. [DOI] [PubMed] [Google Scholar]