Abstract
Sexual dimorphism of mouse complement component 5 (C5) was detected by isoelectric focusing of desialated ethylenediamine tetraacetic acid (EDTA)-plasma on agarose gel, followed by immunofixation with anti- mouse C5. Male plasma displayed two C5 bands, basic and acidic, while female plasma had only a basic C5 band. In all mouse strains tested except C5-deficient strains, the identical patterns of the dimorphism were obtained. The basic and acidic C5 were antigenically and hemolytically indistinguishable from each other. In the neonatal mice of both sexes, the basic C5 was observed at the same levels, but the acidic C5 was only slightly detected. Injection of testosterone into mice resulted in an appearance of the acidic C5 in females and its increase in males. By contrast, injection of estradiol decreased the acidic C5. These results indicate that the expression of the acidic C5 is under the control of testosterone.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Borsos T., Rapp H. J. Immune hemolysis: a simplified method for the preparation of EAC'4 with guinea pig or with human complement. J Immunol. 1967 Aug;99(2):263–268. [PubMed] [Google Scholar]
- CINADER B., DUBISKI S., WARDLAW A. C. DISTRIBUTION, INHERITANCE, AND PROPERTIES OF AN ANTIGEN, MUB1, AND ITS RELATION TO HEMOLYTIC COMPLEMENT. J Exp Med. 1964 Nov 1;120:897–924. doi: 10.1084/jem.120.5.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Churchill W. H., Jr, Weintraub R. M., Borsos T., Rapp H. J. Mouse complement: the effect of sex hormones and castration on two of the late-acting components. J Exp Med. 1967 Apr 1;125(4):657–672. doi: 10.1084/jem.125.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubiski S., Cinader B. Gene dosage effect of serum concentration of a complement component, MuBl. Proc Soc Exp Biol Med. 1966 Jul;122(3):775–778. doi: 10.3181/00379727-122-31249. [DOI] [PubMed] [Google Scholar]
- Ferreira A., Nussenzweig V., Gigli I. Structural and functional differences between the H-2 controlled Ss and Slp proteins. J Exp Med. 1978 Nov 1;148(5):1186–1197. doi: 10.1084/jem.148.5.1186. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferreira A., Weisz-Carrington P., Nussenzweig V. Testosterone control of serum levels of C4-binding protein in mice. J Immunol. 1978 Sep;121(3):1213–1215. [PubMed] [Google Scholar]
- Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
- Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
- Ooi Y. M., Colten H. R. Biosynthesis and post-synthetic modification of a precursor (pro-C5) of the fifth component of mouse complement (C5). J Immunol. 1979 Dec;123(6):2494–2498. [PubMed] [Google Scholar]
- Passmore H. C., Shreffler D. C. A sex-limited serum protein variant in the mouse: hormonal control of phenotypic expression. Biochem Genet. 1971 Apr;5(2):201–209. doi: 10.1007/BF00485645. [DOI] [PubMed] [Google Scholar]
- Shreffler D. C. The S region of the mouse major histocompatibility complex (H-2): genetic variation and functional role in complement system. Transplant Rev. 1976;32:140–167. doi: 10.1111/j.1600-065x.1976.tb00232.x. [DOI] [PubMed] [Google Scholar]
- Tamura N. An incompatibility in the reaction of the second component of human complement with the fourth component of guinea-pig complement. Immunology. 1970 Feb;18(2):203–212. [PMC free article] [PubMed] [Google Scholar]
- Urbach G., Cinader B. Hormonal control of MuBl concentration. Proc Soc Exp Biol Med. 1966 Jul;122(3):779–782. doi: 10.3181/00379727-122-31250. [DOI] [PubMed] [Google Scholar]