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. 1984 Aug 1;160(2):624–629. doi: 10.1084/jem.160.2.624

The three major antigens on the surface of Plasmodium falciparum merozoites are derived from a single high molecular weight precursor

PMCID: PMC2187444  PMID: 6381636

Abstract

A 195,000 mol wt Plasmodium falciparum protein and processing fragments derived from it have been purified by monoclonal antibody affinity chromatography. A polyvalent antiserum has been raised against the purified protein and used to identify the terminal processing products associated with the merozoite. Three unique fragments of 83,000, 42,000, and 19,000 mol wt are present and they represent the major surface antigens of P. falciparum merozoites.

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Selected References

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  1. Cohen S., Butcher G. A., Crandall R. B. Action of malarial antibody in vitro. Nature. 1969 Jul 26;223(5204):368–371. doi: 10.1038/223368a0. [DOI] [PubMed] [Google Scholar]
  2. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  3. Freeman R. R., Holder A. A. Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83,000 mol wt form expressed on the surface of Plasmodium falciparum merozoites. J Exp Med. 1983 Nov 1;158(5):1647–1653. doi: 10.1084/jem.158.5.1647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holder A. A., Freeman R. R. Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody. J Exp Med. 1982 Nov 1;156(5):1528–1538. doi: 10.1084/jem.156.5.1528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Holder A. A., Freeman R. R. Characterization of a high molecular weight protective antigen of Plasmodium yoelii. Parasitology. 1984 Apr;88(Pt 2):211–219. [PubMed] [Google Scholar]
  6. Holder A. A., Freeman R. R., Newbold C. I. Serological cross-reaction between high molecular weight proteins synthesized in blood schizonts of Plasmodium yoelii, Plasmodium chabaudi and Plasmodium falciparum. Mol Biochem Parasitol. 1983 Nov;9(3):191–196. doi: 10.1016/0166-6851(83)90096-8. [DOI] [PubMed] [Google Scholar]
  7. Miller L. H., Aikawa M., Dvorak J. A. Malaria (Plasmodium knowlesi) merozoites: immunity and the surface coat. J Immunol. 1975 Apr;114(4):1237–1242. [PubMed] [Google Scholar]
  8. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]

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