Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1984 Oct 1;160(4):1195–1205. doi: 10.1084/jem.160.4.1195

Syngeneic monoclonal antiidiotype can induce cellular immunity to reovirus

PMCID: PMC2187481  PMID: 6332875

Abstract

A syngeneic monoclonal antiidiotypic antibody was generated in BALB/c mice after repeated immunization with a BALB/c monoclonal anti-reovirus hemagglutinin (HA) antibody. The resultant syngeneic monoclonal antiidiotypic antibody, in the absence of adjuvant, was found to be capable of priming both BALB/c (H-2d, Igh-1a) and C3H/Hej (H-2k, Igh- 1j) mice for Lyt-1+- and Lyt-2+-dependent responses against the mammalian reovirus. By the use of intertypic reassortants and variant virus analysis, the specificity of the response was finely mapped to the neutralization domain of the viral hemagglutinin (HA). Using purified monoclonal antiidiotype, we were able to compare the potency of antiidiotype to virus in terms of induction of immunity. 8 X 10(8) protein molecules were able to prime for cellular responses to reovirus. These studies indicate that in the reovirus system, T cells and B cells share idiotypic configurations, and that antiidiotypic antibodies of the type described herein may be useful in the development of vaccines against certain viral infections.

Full Text

The Full Text of this article is available as a PDF (658.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bluestone J. A., Sharrow S. O., Epstein S. L., Ozato K., Sachs D. H. Induction of anti-H-2 antibodies without alloantigen exposure by in vivo administration of anti-idiotype. Nature. 1981 May 21;291(5812):233–235. doi: 10.1038/291233a0. [DOI] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  3. Burstin S. J., Spriggs D. R., Fields B. N. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology. 1982 Feb;117(1):146–155. doi: 10.1016/0042-6822(82)90514-1. [DOI] [PubMed] [Google Scholar]
  4. Drayna D., Fields B. N. Genetic studies on the mechanism of chemical and physical inactivation of reovirus. J Gen Virol. 1982 Nov;63(Pt 1):149–159. doi: 10.1099/0022-1317-63-1-149. [DOI] [PubMed] [Google Scholar]
  5. Enegren B. J., Burness A. T. Chemical structure of attachment sites for viruses on human erythrocytes. Nature. 1977 Aug 11;268(5620):536–537. doi: 10.1038/268536a0. [DOI] [PubMed] [Google Scholar]
  6. Ertl H. C., Greene M. I., Noseworthy J. H., Fields B. N., Nepom J. T., Spriggs D. R., Finberg R. W. Identification of idiotypic receptors on reovirus-specific cytolytic T cells. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7479–7483. doi: 10.1073/pnas.79.23.7479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ertl H. C., Homans E., Tournas S., Finberg R. W. Sendai virus-specific T cell clones. V. Induction of a virus-specific response by antiidiotypic antibodies directed against a T helper cell clone. J Exp Med. 1984 Jun 1;159(6):1778–1783. doi: 10.1084/jem.159.6.1778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Finberg R., Spriggs D. R., Fields B. N. Host immune response to reovirus: CTL recognize the major neutralization domain of the viral hemagglutinin. J Immunol. 1982 Nov;129(5):2235–2238. [PubMed] [Google Scholar]
  9. Finberg R., Weiner H. L., Fields B. N., Benacerraf B., Burakoff S. J. Generation of cytolytic T lymphocytes after reovirus infection: role of S1 gene. Proc Natl Acad Sci U S A. 1979 Jan;76(1):442–446. doi: 10.1073/pnas.76.1.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Greene M. I., Sugimoto M., Benacerraf B. Mechanisms of regulation of cell-mediated immune responses. I. Effect of the route of immunization with TNP-coupled syngeneic cells on the induction and suppression of contact sensitivity to picryl chloride. J Immunol. 1978 May;120(5):1604–1611. [PubMed] [Google Scholar]
  11. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  12. Kauffman R. S., Noseworthy J. H., Nepom J. T., Finberg R., Fields B. N., Greene M. I. Cell receptors for the mammalian reovirus. II. Monoclonal anti-idiotypic antibody blocks viral binding to cells. J Immunol. 1983 Nov;131(5):2539–2541. [PubMed] [Google Scholar]
  13. Kennedy R. C., Adler-Storthz K., Henkel R. D., Sanchez Y., Melnick J. L., Dreesman G. R. Immune response to hepatitis B surface antigen: enhancement by prior injection of antibodies to the idiotype. Science. 1983 Aug 26;221(4613):853–855. doi: 10.1126/science.6603657. [DOI] [PubMed] [Google Scholar]
  14. Kennedy R. C., Dreesman G. R. Enhancement of the immune response to hepatitis B surface antigen. In vivo administration of antiidiotype induces anti-HBs that expresses a similar idiotype. J Exp Med. 1984 Mar 1;159(3):655–665. doi: 10.1084/jem.159.3.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lee P. W., Hayes E. C., Joklik W. K. Protein sigma 1 is the reovirus cell attachment protein. Virology. 1981 Jan 15;108(1):156–163. doi: 10.1016/0042-6822(81)90535-3. [DOI] [PubMed] [Google Scholar]
  16. McClintock P. R., Billups L. C., Notkins A. L. Receptors for encephalomyocarditis virus on murine and human cells. Virology. 1980 Oct 30;106(2):261–272. doi: 10.1016/0042-6822(80)90249-4. [DOI] [PubMed] [Google Scholar]
  17. McLAREN L. C., HOLLAND J. J., SYVERTON J. T. The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J Exp Med. 1959 May 1;109(5):475–485. doi: 10.1084/jem.109.5.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nisonoff A., Lamoyi E. Implications of the presence of an internal image of the antigen in anti-idiotypic antibodies: possible application to vaccine production. Clin Immunol Immunopathol. 1981 Dec;21(3):397–406. doi: 10.1016/0090-1229(81)90228-2. [DOI] [PubMed] [Google Scholar]
  19. Noseworthy J. H., Fields B. N., Dichter M. A., Sobotka C., Pizer E., Perry L. L., Nepom J. T., Greene M. I. Cell receptors for the mammalian reovirus. I. Syngeneic monoclonal anti-idiotypic antibody identifies a cell surface receptor for reovirus. J Immunol. 1983 Nov;131(5):2533–2538. [PubMed] [Google Scholar]
  20. Philipson L., Lonberg-Holm K., Pettersson U. Virus-receptor interaction in an adenovirus system. J Virol. 1968 Oct;2(10):1064–1075. doi: 10.1128/jvi.2.10.1064-1075.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ramig R. F., Cross R. K., Fields B. N. Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3. J Virol. 1977 Jun;22(3):726–733. doi: 10.1128/jvi.22.3.726-733.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roth R. A., Cassell D. J., Wong K. Y., Maddux B. A., Goldfine I. D. Monoclonal antibodies to the human insulin receptor block insulin binding and inhibit insulin action. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7312–7316. doi: 10.1073/pnas.79.23.7312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sharpe A. H., Fields B. N. Reovirus inhibition of cellular RNA and protein synthesis: role of the S4 gene. Virology. 1982 Oct 30;122(2):381–391. doi: 10.1016/0042-6822(82)90237-9. [DOI] [PubMed] [Google Scholar]
  24. Spriggs D. R., Bronson R. T., Fields B. N. Hemagglutinin variants of reovirus type 3 have altered central nervous system tropism. Science. 1983 Apr 29;220(4596):505–507. doi: 10.1126/science.6301010. [DOI] [PubMed] [Google Scholar]
  25. Spriggs D. R., Kaye K., Fields B. N. Topological analysis of the reovirus type 3 hemagglutinin. Virology. 1983 May;127(1):220–224. doi: 10.1016/0042-6822(83)90385-9. [DOI] [PubMed] [Google Scholar]
  26. Tardieu M., Weiner H. L. Viral receptors on isolated murine and human ependymal cells. Science. 1982 Jan 22;215(4531):419–421. doi: 10.1126/science.6276976. [DOI] [PubMed] [Google Scholar]
  27. Weiner H. L., Ault K. A., Fields B. N. Interaction of reovirus with cell surface receptors. I. Murine and human lymphocytes have a receptor for the hemagglutinin of reovirus type 3. J Immunol. 1980 May;124(5):2143–2148. [PubMed] [Google Scholar]
  28. Weiner H. L., Drayna D., Averill D. R., Jr, Fields B. N. Molecular basis of reovirus virulence: role of the S1 gene. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5744–5748. doi: 10.1073/pnas.74.12.5744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Weiner H. L., Greene M. I., Fields B. N. Delayed hypersensitivity in mice infected with reovirus. I. Identification of host and viral gene products responsible for the immune response. J Immunol. 1980 Jul;125(1):278–282. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES