Abstract
Children with autoimmune chronic active hepatitis may have high titers of antibodies detected by immunofluorescence staining of hepatocytes and tubular cells in rat liver and kidney sections, respectively. These antibodies are directed against antigens contained in microsomal fractions prepared from these two organs. We have found that sera from these patients recognized a 50,000 mol wt protein present in higher concentration in smooth microsome subfractions compared with rough microsome subfractions. This protein is an integral membrane protein and is not glycosylated. It is exposed on the cytoplasmic face of the endoplasmic reticulum and is rather resistant to proteolysis with proteinase K. Since patients with liver disease of different etiology and similar severity of cell lysis do not give rise to liver-kidney microsome antibody (LKMA), lysis of hepatocytes is apparently not a sufficient condition for their development.
Full Text
The Full Text of this article is available as a PDF (612.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Amar-Costesec A., Todd J. A., Kreibich G. Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. I. Functional tests on rat liver microsomal subfractions. J Cell Biol. 1984 Dec;99(6):2247–2253. doi: 10.1083/jcb.99.6.2247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ballardini G., Landi P., Busachi C. A., Bianchi F. B., Pisi E. HBsAg-induced hypertrophic smooth endoplasmic reticulum as a target for liver-kidney microsomal (LKM) antibodies. Clin Exp Immunol. 1981 Mar;43(3):599–604. [PMC free article] [PubMed] [Google Scholar]
- Crivelli O., Lavarini C., Chiaberge E., Amoroso A., Farci P., Negro F., Rizzetto M. Microsomal autoantibodies in chronic infection with the HBsAg associated delta (delta) agent. Clin Exp Immunol. 1983 Oct;54(1):232–238. [PMC free article] [PubMed] [Google Scholar]
- Frey A. B., Rosenfeld M. G., Dolan W. J., Adesnik M., Kreibich G. Induction of cytochrome P-450 isozymes in rat hepatoma-derived cell cultures. J Cell Physiol. 1984 Aug;120(2):169–180. doi: 10.1002/jcp.1041200210. [DOI] [PubMed] [Google Scholar]
- Fujiki Y., Hubbard A. L., Fowler S., Lazarow P. B. Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biol. 1982 Apr;93(1):97–102. doi: 10.1083/jcb.93.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Homberg J. C., Andre C., Abuaf N. A new anti-liver-kidney microsome antibody (anti-LKM2) in tienilic acid-induced hepatitis. Clin Exp Immunol. 1984 Mar;55(3):561–570. [PMC free article] [PubMed] [Google Scholar]
- Kreibich G., Debey P., Sabatini D. D. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations. J Cell Biol. 1973 Aug;58(2):436–462. doi: 10.1083/jcb.58.2.436. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kruppa J., Sabatini D. D. Release of poly A(+) messenger RNA from rat liver rough microsomes upon disassembly of bound polysomes. J Cell Biol. 1977 Aug;74(2):414–427. doi: 10.1083/jcb.74.2.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Obrink B. Hepatocyte--collagen adhesion. Methods Enzymol. 1982;82(Pt A):513–529. doi: 10.1016/0076-6879(82)82083-1. [DOI] [PubMed] [Google Scholar]
- Odièvre M., Maggiore G., Homberg J. C., Saadoun F., Couroucé A. M., Yvart J., Hadchouel M., Alagille D. Seroimmunologic classification of chronic hepatitis in 57 children. Hepatology. 1983 May-Jun;3(3):407–409. doi: 10.1002/hep.1840030320. [DOI] [PubMed] [Google Scholar]
- Rizzetto M., Bianchi F. B., Doniach D. Characterization of the microsomal antigen related to a subclass of active chronic hepatitis. Immunology. 1974 Mar;26(3):589–601. [PMC free article] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]