Abstract
Two T4 cell clones (TLC) specific for antigenic epitopes on Chlamydia trachomatis were studied. Using a panel of allogeneic antigen- presenting cells (APC), both TLC were found to be restricted by HLA class II elements closely associated with, but not identical to the DRw5S specificity, as determined by highly selected alloantisera, a monoclonal antibody (mAb), 109d6, and confirmed on the DNA level by determination of restriction fragment length polymorphisms (RFLP) with a DR beta probe. Furthermore, HLA-DR-specific mAb, including 109d6, but not other HLA class II- or class I-specific antibodies inhibited the two TLC, strongly suggesting that the restriction element is expressed by a DR molecule. Using digestion with Hind III restriction enzyme and a DR beta probe, we found a complete concordance between the appearance of a 9.3 kilobase band and the ability of allogeneic APC to restimulate the T cell clones. Thus, the restriction element for these T cell clones appear to be expressed by DR molecules, but can, at present, only be detected at the genomic level.
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Selected References
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- Ball E. J., Stastny P. Antigen-specific HLA-restricted human T-cell lines. II. A GAT-specific T-cell line restricted by a determinant carried by an HLA-DQ molecule. Immunogenetics. 1984;20(5):547–564. doi: 10.1007/BF00364357. [DOI] [PubMed] [Google Scholar]
- Gustafsson K., Wiman K., Emmoth E., Larhammar D., Böhme J., Hyldig-Nielsen J. J., Ronne H., Peterson P. A., Rask L. Mutations and selection in the generation of class II histocompatibility antigen polymorphism. EMBO J. 1984 Jul;3(7):1655–1661. doi: 10.1002/j.1460-2075.1984.tb02026.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heber-Katz E., Hansburg D., Schwartz R. H. The Ia molecule of the antigen-presenting cell plays a critical role in immune response gene regulation of T cell activation. J Mol Cell Immunol. 1983;1(1):3–18. [PubMed] [Google Scholar]
- Hurley C. K., Giles R. C., Nunez G., DeMars R., Nadler L., Winchester R., Stastny P., Capra J. D. Molecular localization of human class II MT2 and MT3 determinants. J Exp Med. 1984 Aug 1;160(2):472–493. doi: 10.1084/jem.160.2.472. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moen T., Bratlie A., Kiss E., Bruserud O., Thorsby E. Identification of four SB antigens by cloned cells. Population studies of Norwegians. Tissue Antigens. 1983 Oct;22(4):298–309. doi: 10.1111/j.1399-0039.1983.tb01207.x. [DOI] [PubMed] [Google Scholar]
- Qvigstad E., Digranes S., Thorsby E. Antigen-specific proliferative human T-lymphocyte clones with specificity for Chlamydia trachomatis. Scand J Immunol. 1983 Oct;18(4):291–297. doi: 10.1111/j.1365-3083.1983.tb01800.x. [DOI] [PubMed] [Google Scholar]
- Qvigstad E., Gaudernack G., Thorsby E. Antigen-specific T cell clones restricted by DR, DRw53 (MT), or DP (SB) Class II HLA molecules. Inhibition studies with monoclonal HLA-specific antibodies. Hum Immunol. 1984 Dec;11(4):207–217. doi: 10.1016/0198-8859(84)90060-0. [DOI] [PubMed] [Google Scholar]
- Qvigstad E., Moen T., Thorsby E. T-cell clones with similar antigen specificity may be restricted by DR, MT(DC), or SB class II HLA molecules. Immunogenetics. 1984;19(5):455–460. doi: 10.1007/BF00364648. [DOI] [PubMed] [Google Scholar]
- Qvigstad E., Scott H., Thorsby E. HLA class-II restriction of antigen-specific T-cell activation. With special reference to the fine specificity and immunoregulation of HLA class-II restriction elements. Prog Allergy. 1985;36:73–94. [PubMed] [Google Scholar]
- Qvigstad E., Thorsby E. Class-II HLA restriction of antigen-specific human T-lymphocyte clones. Evidence of restriction elements on both DR and MT molecules. Scand J Immunol. 1983 Oct;18(4):299–306. doi: 10.1111/j.1365-3083.1983.tb01801.x. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Toguchi T., Burmester G., Nunez-Roldan A., Gregersen P., Seremetis S., Lee S., Szer I., Winchester R. Evidence for the separate molecular expression of four distinct polymorphic Ia epitopes on cells of DR4 homozygous individuals. Hum Immunol. 1984 Jun;10(2):69–81. doi: 10.1016/0198-8859(84)90074-0. [DOI] [PubMed] [Google Scholar]