Abstract
The chromosomal location of Ti alpha was determined by hybridization of a radiolabeled cDNA for the alpha chain of human T cell receptor with 12 human X mouse cell hybrid DNAs cleaved with BamHI. Seven hybrids contained human Ti alpha, while the remaining five lacked it. Only human chromosome 14 matched the distribution of human Ti alpha signal across the mapping panel. Hybrids segregating a chromosome 14 translocation were used to demonstrate that Ti alpha is in the region 14pter greater than 14q21. Thus, the alpha and beta chain genes that contribute structural components to the Ti moiety of the human T cell receptor lie on different chromosomes. In humans, the immunoglobulin heavy chain locus and Ti alpha are in different regions of chromosome 14, with Ti alpha more proximal and the immunoglobulin heavy chain locus more distal.
Full Text
The Full Text of this article is available as a PDF (531.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Acuto O., Reinherz E. L. The human T-cell receptor. Structure and function. N Engl J Med. 1985 Apr 25;312(17):1100–1111. doi: 10.1056/NEJM198504253121706. [DOI] [PubMed] [Google Scholar]
- Barker P. E., Rabin M., Watson M., Breg W. R., Ruddle F. H., Verma I. M. Human c-fos oncogene mapped within chromosomal region 14q21----q31. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5826–5830. doi: 10.1073/pnas.81.18.5826. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barker P. E., Ruddle F. H., Royer H. D., Acuto O., Reinherz E. L. Chromosomal location of human T-cell receptor gene Ti beta. Science. 1984 Oct 19;226(4672):348–349. doi: 10.1126/science.6435246. [DOI] [PubMed] [Google Scholar]
- Caccia N., Kronenberg M., Saxe D., Haars R., Bruns G. A., Goverman J., Malissen M., Willard H., Yoshikai Y., Simon M. The T cell receptor beta chain genes are located on chromosome 6 in mice and chromosome 7 in humans. Cell. 1984 Jul;37(3):1091–1099. doi: 10.1016/0092-8674(84)90443-4. [DOI] [PubMed] [Google Scholar]
- Collins M. K., Goodfellow P. N., Dunne M. J., Spurr N. K., Solomon E., Owen M. J. A human T-cell antigen receptor beta chain gene maps to chromosome 7. EMBO J. 1984 Oct;3(10):2347–2349. doi: 10.1002/j.1460-2075.1984.tb02137.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Croce C. M., Shander M., Martinis J., Cicurel L., D'Ancona G. G., Dolby T. W., Koprowski H. Chromosomal location of the genes for human immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3416–3419. doi: 10.1073/pnas.76.7.3416. [DOI] [PMC free article] [PubMed] [Google Scholar]
- D'Eustachio P. A genetic map of mouse chromosome 12 composed of polymorphic DNA fragments. J Exp Med. 1984 Sep 1;160(3):827–838. doi: 10.1084/jem.160.3.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- D'Eustachio P., Pravtcheva D., Marcu K., Ruddle F. H. Chromosomal location of the structural gene cluster encoding murine immunoglobulin heavy chains. J Exp Med. 1980 Jun 1;151(6):1545–1550. doi: 10.1084/jem.151.6.1545. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dalla-Favera R., Bregni M., Erikson J., Patterson D., Gallo R. C., Croce C. M. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc Natl Acad Sci U S A. 1982 Dec;79(24):7824–7827. doi: 10.1073/pnas.79.24.7824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donlon T. A., Litt M., Newcom S. R., Magenis R. E. Localization of the restriction fragment length polymorphism D14S1 (pAW-101) to chromosome 14q32.1 leads to 32.2 by in situ hybridization. Am J Hum Genet. 1983 Nov;35(6):1097–1106. [PMC free article] [PubMed] [Google Scholar]
- Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
- Giblett E. R., Ammann A. J., Wara D. W., Sandman R., Diamond L. K. Nucleoside-phosphorylase deficiency in a child with severely defective T-cell immunity and normal B-cell immunity. Lancet. 1975 May 3;1(7914):1010–1013. doi: 10.1016/s0140-6736(75)91950-9. [DOI] [PubMed] [Google Scholar]
- Hecht F., Morgan R., Hecht B. K., Smith S. D. Common region on chromosome 14 in T-cell leukemia and lymphoma. Science. 1984 Dec 21;226(4681):1445–1447. doi: 10.1126/science.6438800. [DOI] [PubMed] [Google Scholar]
- Kranz D. M., Saito H., Disteche C. M., Swisshelm K., Pravtcheva D., Ruddle F. H., Eisen H. N., Tonegawa S. Chromosomal locations of the murine T-cell receptor alpha-chain gene and the T-cell gamma gene. Science. 1985 Feb 22;227(4689):941–945. doi: 10.1126/science.3918347. [DOI] [PubMed] [Google Scholar]
- Lee N. E., D'Eustachio P., Pravtcheva D., Ruddle F. H., Hedrick S. M., Davis M. M. Murine T cell receptor beta chain is encoded on chromosome 6. J Exp Med. 1984 Sep 1;160(3):905–913. doi: 10.1084/jem.160.3.905. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swan D., D'Eustachio P., Leinwand L., Seidman J., Keithley D., Ruddle F. H. Chromosomal assignment of the mouse kappa light chain genes. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2735–2739. doi: 10.1073/pnas.76.6.2735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams D. L., Look A. T., Melvin S. L., Roberson P. K., Dahl G., Flake T., Stass S. New chromosomal translocations correlate with specific immunophenotypes of childhood acute lymphoblastic leukemia. Cell. 1984 Jan;36(1):101–109. doi: 10.1016/0092-8674(84)90078-3. [DOI] [PubMed] [Google Scholar]
- Yunis J. J., Soreng A. L. Constitutive fragile sites and cancer. Science. 1984 Dec 7;226(4679):1199–1204. doi: 10.1126/science.6239375. [DOI] [PubMed] [Google Scholar]
- de Klein A., van Kessel A. G., Grosveld G., Bartram C. R., Hagemeijer A., Bootsma D., Spurr N. K., Heisterkamp N., Groffen J., Stephenson J. R. A cellular oncogene is translocated to the Philadelphia chromosome in chronic myelocytic leukaemia. Nature. 1982 Dec 23;300(5894):765–767. doi: 10.1038/300765a0. [DOI] [PubMed] [Google Scholar]