Abstract
We present here the molecular characterization of a new activation- induced surface structure on human T lymphocytes, termed LA45, with high homology (93% at protein level) to MHC class I molecules. Antigen modulation and sequential immunoprecipitation experiments revealed that LA45 and HLA class I proteins do not crossreact with the corresponding antibodies. Furthermore, LA45 is not associated with beta 2-m. On the other hand, we could show that the separation of HLA-A,B,C and beta 2m molecules, induced by SDS-denaturation, leads to a conformational change in the heavy chain in such a way that it becomes reactive with LA45. The 90/45 kD LA45 proteins thus appear to be non-beta 2m- associated MHC class I alpha chains that are selectively expressed by activated but not by resting human T lymphocytes.
Full Text
The Full Text of this article is available as a PDF (753.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allen H., Fraser J., Flyer D., Calvin S., Flavell R. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7447–7451. doi: 10.1073/pnas.83.19.7447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aruffo A., Seed B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8573–8577. doi: 10.1073/pnas.84.23.8573. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beck J. C., Hansen T. H., Cullen S. E., Lee D. R. Slower processing, weaker beta 2-M association, and lower surface expression of H-2Ld are influenced by its amino terminus. J Immunol. 1986 Aug 1;137(3):916–923. [PubMed] [Google Scholar]
- Bernabeu C., van de Rijn M., Lerch P. G., Terhorst C. P. Beta 2-microglobulin from serum associates with MHC class I antigens on the surface of cultured cells. Nature. 1984 Apr 12;308(5960):642–645. doi: 10.1038/308642a0. [DOI] [PubMed] [Google Scholar]
- Bjorkman P. J., Saper M. A., Samraoui B., Bennett W. S., Strominger J. L., Wiley D. C. Structure of the human class I histocompatibility antigen, HLA-A2. Nature. 1987 Oct 8;329(6139):506–512. doi: 10.1038/329506a0. [DOI] [PubMed] [Google Scholar]
- Due C., Simonsen M., Olsson L. The major histocompatibility complex class I heavy chain as a structural subunit of the human cell membrane insulin receptor: implications for the range of biological functions of histocompatibility antigens. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6007–6011. doi: 10.1073/pnas.83.16.6007. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fauchet R., Boscher M., Bouhallier O., Merdrignac G., Genetet B., Turmel P., Charron D. J. New class I in man: serological and molecular characterization. Hum Immunol. 1986 Sep;17(1):3–20. doi: 10.1016/0198-8859(86)90069-8. [DOI] [PubMed] [Google Scholar]
- Gazit E., Gothelf Y., Gil R., Orgad S., Pitman T. B., Watson A. L., Yang S. Y., Yunis E. J. Alloantibodies to PHA-activated lymphocytes detect human Qa-like antigens. J Immunol. 1984 Jan;132(1):165–169. [PubMed] [Google Scholar]
- Gubler U., Hoffman B. J. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. doi: 10.1016/0378-1119(83)90230-5. [DOI] [PubMed] [Google Scholar]
- Holter W., Grunow R., Stockinger H., Knapp W. Recombinant interferon-gamma induces interleukin 2 receptors on human peripheral blood monocytes. J Immunol. 1986 Mar 15;136(6):2171–2175. [PubMed] [Google Scholar]
- Koller B. H., Geraghty D. E., Shimizu Y., DeMars R., Orr H. T. HLA-E. A novel HLA class I gene expressed in resting T lymphocytes. J Immunol. 1988 Aug 1;141(3):897–904. [PubMed] [Google Scholar]
- Krangel M. S., Orr H. T., Strominger J. L. Assembly and maturation of HLA-A and HLA-B antigens in vivo. Cell. 1979 Dec;18(4):979–991. doi: 10.1016/0092-8674(79)90210-1. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lancet D., Parham P., Strominger J. L. Heavy chain of HLA-A and HLA-B antigens is conformationally labile: a possible role for beta 2-microglobulin. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3844–3848. doi: 10.1073/pnas.76.8.3844. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipman D. J., Pearson W. R. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. doi: 10.1126/science.2983426. [DOI] [PubMed] [Google Scholar]
- Miller J., Germain R. N. Efficient cell surface expression of class II MHC molecules in the absence of associated invariant chain. J Exp Med. 1986 Nov 1;164(5):1478–1489. doi: 10.1084/jem.164.5.1478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitsuishi Y., Falkenrodt A., Urlacher A., Tongio M. M., Mayer S. New human MHC class I antigens segregating with HLA-A antigens detected on some lymphocyte subpopulations. Hum Immunol. 1986 Feb;15(2):175–197. doi: 10.1016/0198-8859(86)90025-x. [DOI] [PubMed] [Google Scholar]
- Mizuno S., Trapani J. A., Koller B. H., Dupont B., Yang S. Y. Isolation and nucleotide sequence of a cDNA clone encoding a novel HLA class I gene. J Immunol. 1988 Jun 1;140(11):4024–4030. [PubMed] [Google Scholar]
- Parham P., Barnstable C. J., Bodmer W. F. Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C, antigens. J Immunol. 1979 Jul;123(1):342–349. [PubMed] [Google Scholar]
- Parham P., Lawlor D. A., Lomen C. E., Ennis P. D. Diversity and diversification of HLA-A,B,C alleles. J Immunol. 1989 Jun 1;142(11):3937–3950. [PubMed] [Google Scholar]
- Parham P., Lomen C. E., Lawlor D. A., Ways J. P., Holmes N., Coppin H. L., Salter R. D., Wan A. M., Ennis P. D. Nature of polymorphism in HLA-A, -B, and -C molecules. Proc Natl Acad Sci U S A. 1988 Jun;85(11):4005–4009. doi: 10.1073/pnas.85.11.4005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paul P., Fauchet R., Boscher M. Y., Sayagh B., Masset M., Medrignac G., Dausset J., Cohen D. Isolation of a human major histocompatibility complex class I gene encoding a nonubiquitous molecule expressed on activated lymphocytes. Proc Natl Acad Sci U S A. 1987 May;84(9):2872–2876. doi: 10.1073/pnas.84.9.2872. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ploegh H. L., Orr H. T., Strominger J. L. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules. Cell. 1981 May;24(2):287–299. doi: 10.1016/0092-8674(81)90318-4. [DOI] [PubMed] [Google Scholar]
- Robb R. J., Greene W. C., Rusk C. M. Low and high affinity cellular receptors for interleukin 2. Implications for the level of Tac antigen. J Exp Med. 1984 Oct 1;160(4):1126–1146. doi: 10.1084/jem.160.4.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Samson M., Cousin J. L., Fehlmann M. Cross-linking of insulin receptors to MHC antigens in human B lymphocytes: evidence for selective molecular interactions. J Immunol. 1986 Oct 1;137(7):2293–2298. [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schreiber A. B., Schlessinger J., Edidin M. Interaction between major histocompatibility complex antigens and epidermal growth factor receptors on human cells. J Cell Biol. 1984 Feb;98(2):725–731. doi: 10.1083/jcb.98.2.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Short J. M., Fernandez J. M., Sorge J. A., Huse W. D. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res. 1988 Aug 11;16(15):7583–7600. doi: 10.1093/nar/16.15.7583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Srivastava R., Chorney M. J., Lawrance S. K., Pan J., Smith Z., Smith C. L., Weissman S. M. Structure, expression, and molecular mapping of a divergent member of the class I HLA gene family. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4224–4228. doi: 10.1073/pnas.84.12.4224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stam N. J., Spits H., Ploegh H. L. Monoclonal antibodies raised against denatured HLA-B locus heavy chains permit biochemical characterization of certain HLA-C locus products. J Immunol. 1986 Oct 1;137(7):2299–2306. [PubMed] [Google Scholar]