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. 1986 Feb 1;163(2):436–451. doi: 10.1084/jem.163.2.436

Dendritic cells with antigen-presenting capability reside in airway epithelium, lung parenchyma, and visceral pleura

PMCID: PMC2188026  PMID: 3511172

Abstract

In this study, we identified a population of dendritic cells (DC) that exists throughout human and mouse pulmonary tissues, including the trachea, bronchi, alveoli, and visceral pleura. In human tissue, these DC were shown to be positive for HLA-DR and T200 antigens. In the mouse, the DC expressed not only Ia and the T200 antigen, but also Fc- IgG and C3bi receptors. Unlike alveolar macrophages, the DC were negative for nonspecific esterase staining and shared ultrastructural similarities with the DC described by Steinman (1), and with Langerhans' cells, even though they did not contain Birbeck granules. We were able to demonstrate that mouse pulmonary DC function in antigen presentation, as observed with the other DC. Thus, the respiratory tract contains DC that are capable of functioning in antigen presentation and that may be important in pulmonary immune responses.

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Selected References

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  1. Ansfield M. J., Kaltreider H. B., Caldwell J. L., Herskowitz F. N. Hyporesponsiveness of canine bronchoalveolar lymphocytes to mitogens: inhibition of lymphocyte proliferation by alveolar macrophages. J Immunol. 1979 Feb;122(2):542–548. [PubMed] [Google Scholar]
  2. Ashwell J. D., DeFranco A. L., Paul W. E., Schwartz R. H. Can resting B cells present antigen to T cells? Fed Proc. 1985 May;44(8):2475–2479. [PubMed] [Google Scholar]
  3. Bhattacharya A., Dorf M. E., Springer T. A. A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec;127(6):2488–2495. [PubMed] [Google Scholar]
  4. Breeze R. G., Wheeldon E. B. The cells of the pulmonary airways. Am Rev Respir Dis. 1977 Oct;116(4):705–777. doi: 10.1164/arrd.1977.116.4.705. [DOI] [PubMed] [Google Scholar]
  5. Burke C. M., Theodore J., Dawkins K. D., Yousem S. A., Blank N., Billingham M. E., Van Kessel A., Jamieson S. W., Oyer P. E., Baldwin J. C. Post-transplant obliterative bronchiolitis and other late lung sequelae in human heart-lung transplantation. Chest. 1984 Dec;86(6):824–829. doi: 10.1378/chest.86.6.824. [DOI] [PubMed] [Google Scholar]
  6. Daar A. S., Fuggle S. V., Fabre J. W., Ting A., Morris P. J. The detailed distribution of MHC Class II antigens in normal human organs. Transplantation. 1984 Sep;38(3):293–298. doi: 10.1097/00007890-198409000-00019. [DOI] [PubMed] [Google Scholar]
  7. Faustman D. L., Steinman R. M., Gebel H. M., Hauptfeld V., Davie J. M., Lacy P. E. Prevention of rejection of murine islet allografts by pretreatment with anti-dendritic cell antibody. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3864–3868. doi: 10.1073/pnas.81.12.3864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Forsum U., Klareskog L., Peterson P. A. Distribution of Ia-antigen-like molecules on non-lymphoid tissues. Scand J Immunol. 1979;9(4):343–349. doi: 10.1111/j.1365-3083.1979.tb03172.x. [DOI] [PubMed] [Google Scholar]
  9. Glimcher L. H., Shevach E. M. Production of autoreactive I region-restricted T cell hybridomas. J Exp Med. 1982 Aug 1;156(2):640–645. doi: 10.1084/jem.156.2.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirschberg H., Scott H., Thorsby E. Human endothelial cells can present antigen to sensitized T lymphocytes in vitro. Transplant Proc. 1981 Mar;13(1 Pt 1):100–102. [PubMed] [Google Scholar]
  11. Holt P. G. Alveolar macrophages. II. Inhibition of lymphocyte proliferation by purified macrophages from rat lung. Immunology. 1979 Jun;37(2):429–436. [PMC free article] [PubMed] [Google Scholar]
  12. Kawanami O., Basset F., Ferrans V. J., Soler P., Crystal R. G. Pulmonary Langerhans' cells in patients with fibrotic lung disorders. Lab Invest. 1981 Mar;44(3):227–233. [PubMed] [Google Scholar]
  13. Klareskog L., Tjernlund U., Forsum U., Peterson P. A. Epidermal Langerhans cells express Ia antigens. Nature. 1977 Jul 21;268(5617):248–250. doi: 10.1038/268248a0. [DOI] [PubMed] [Google Scholar]
  14. Lanier L. L., Gutman G. A., Lewis D. E., Griswold S. T., Warner N. L. Monoclonal antibodies against rat immunoglobulin kappa chains. Hybridoma. 1982;1(2):125–131. doi: 10.1089/hyb.1.1982.1.125. [DOI] [PubMed] [Google Scholar]
  15. Lyons C. R., Lipscomb M. F. Alveolar macrophages in pulmonary immune responses. I. Role in the initiation of primary immune responses and in the selective recruitment of T lymphocytes to the lung. J Immunol. 1983 Mar;130(3):1113–1119. [PubMed] [Google Scholar]
  16. Malek T. R., Schmidt J. A., Shevach E. M. The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations. J Immunol. 1985 Apr;134(4):2405–2413. [PubMed] [Google Scholar]
  17. Norcross M. A., Bentley D. M., Margulies D. H., Germain R. N. Membrane Ia expression and antigen-presenting accessory cell function of L cells transfected with class II major histocompatibility complex genes. J Exp Med. 1984 Nov 1;160(5):1316–1337. doi: 10.1084/jem.160.5.1316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nunez G., Ball E. J., Stastny P. Accessory cell function of human endothelial cells. I. A subpopulation of Ia positive cells is required for antigen presentation. J Immunol. 1983 Aug;131(2):666–673. [PubMed] [Google Scholar]
  19. Rowden G., Lewis M. G., Sullivan A. K. Ia antigen expression on human epidermal Langerhans cells. Nature. 1977 Jul 21;268(5617):247–248. doi: 10.1038/268247a0. [DOI] [PubMed] [Google Scholar]
  20. Schuler G., Steinman R. M. Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J Exp Med. 1985 Mar 1;161(3):526–546. doi: 10.1084/jem.161.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schuyler M. R., Todd L. S. Accessory cell function of rabbit alveolar macrophages. Am Rev Respir Dis. 1981 Jan;123(1):53–57. doi: 10.1164/arrd.1981.123.1.53. [DOI] [PubMed] [Google Scholar]
  22. Springer T., Galfrè G., Secher D. S., Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978 Aug;8(8):539–551. doi: 10.1002/eji.1830080802. [DOI] [PubMed] [Google Scholar]
  23. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
  24. Stein-Streilein J. Allergic contact dermatitis induced by intratracheal administration of hapten. J Immunol. 1983 Oct;131(4):1748–1753. [PubMed] [Google Scholar]
  25. Steinman R. M., Nussenzweig M. C. Dendritic cells: features and functions. Immunol Rev. 1980;53:127–147. doi: 10.1111/j.1600-065x.1980.tb01042.x. [DOI] [PubMed] [Google Scholar]
  26. Stingl G., Katz S. I., Shevach E. M., Rosenthal A. S., Green I. Analogous functions of macrophages and Langerhans cells in the initiation in the immune response. J Invest Dermatol. 1978 Jul;71(1):59–64. doi: 10.1111/1523-1747.ep12544055. [DOI] [PubMed] [Google Scholar]
  27. Stingl G., Wolff-Schreiner E. C., Pichler W. J., Gschnait F., Knapp W., Wolff K. Epidermal Langerhans cells bear Fc and C3 receptors. Nature. 1977 Jul 21;268(5617):245–246. doi: 10.1038/268245a0. [DOI] [PubMed] [Google Scholar]
  28. Tandler B., Sherman J., Boat T. F. EDTA-mediated separation of cat tracheal lining epithelium. Am Rev Respir Dis. 1981 Oct;124(4):469–475. doi: 10.1164/arrd.1981.124.4.469. [DOI] [PubMed] [Google Scholar]
  29. Toews G. B., Vial W. C., Dunn M. M., Guzzetta P., Nunez G., Stastny P., Lipscomb M. F. The accessory cell function of human alveolar macrophages in specific T cell proliferation. J Immunol. 1984 Jan;132(1):181–186. [PubMed] [Google Scholar]
  30. Unkeless J. C. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3):580–596. doi: 10.1084/jem.150.3.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Volc-Platzer B., Majdic O., Knapp W., Wolff K., Hinterberger W., Lechner K., Stingl G. Evidence of HLA-DR antigen biosynthesis by human keratinocytes in disease. J Exp Med. 1984 Jun 1;159(6):1784–1789. doi: 10.1084/jem.159.6.1784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Weinberg D. S., Unanue E. R. Antigen-presenting function of alveolar macrophages: uptake and presentation of Listeria monocytogenes. J Immunol. 1981 Feb;126(2):794–799. [PubMed] [Google Scholar]
  33. Wiman K., Curman B., Forsum U., Klareskog L., Malmnäs-Tjernlund U., Rask L., Trägårdh L., Peterson P. A. Occurrence of Ia antigens on tissues on non-lymphoid origin. Nature. 1978 Dec 14;276(5689):711–713. doi: 10.1038/276711a0. [DOI] [PubMed] [Google Scholar]
  34. Wyatt S. E., Nunn P., Hows J. M., Yin J., Hayes M. C., Catovsky D., Gordon-Smith E. C., Hughes J. M., Goldman J. M., Galton D. Airways obstruction associated with graft versus host disease after bone marrow transplantation. Thorax. 1984 Dec;39(12):887–894. doi: 10.1136/thx.39.12.887. [DOI] [PMC free article] [PubMed] [Google Scholar]

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