Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1986 Mar 1;163(3):588–602. doi: 10.1084/jem.163.3.588

Antibodies to basement membrane collagen and to laminin are present in sera from patients with poststreptococcal glomerulonephritis

PMCID: PMC2188061  PMID: 3950543

Abstract

Sera from patients with poststreptococcal glomerulonephritis (PSGN) known to have antibodies to proteoglycans were studied for the presence of antibodies against other basement membrane (BM) components. BM collagen (type IV) was isolated in the native state by extracting bovine anterior lens capsule (ALC) with 0.5 M acetic acid. The 7-S (collagenous) domain and the NC-1 (noncollagenous) domain of type IV collagen were obtained after bacterial collagenase digestion of ALC followed by gel filtration. Laminin was isolated from the mouse EHS tumor and fibronectin from human plasma. Immunologic studies, using an ELISA and electroimmunoblot, revealed the presence of antibodies that reacted with intact, native type IV collagen and the 7-S collagenous domain of this molecule. Reaction with the NC-1 (noncollagenous) domain was minimal, and not higher than that obtained with control sera. Laminin reaction strongly with the patients' sera, but fibronectin did not. Unlike sera from patients with Goodpasture syndrome, which contain antibodies primarily against the NC-1 (noncollagenous) domain of type IV collagen, sera from patients with acute PSGN contain antibodies against all the major macromolecular components of BM. This difference in immunologic reactivity may account for the observed differences in the pathologic picture at the glomerular level.

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andres G. A., Accinni L., Hsu K. C., Zabriskie J. B., Seegal B. C. Electron microscopic studies of human glomerulonephritis with ferritin-conjugated antibody. Localization of antigen-antibody complexes in glomerular structures of patients with acute glomerulonephritis. J Exp Med. 1966 Feb 1;123(2):399–412. doi: 10.1084/jem.123.2.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brinker J. M., Pegg M. T., Howard P. S., Kefalides N. A. Immunochemical characterization of type IV procollagen from anterior lens capsule. Coll Relat Res. 1985 Jun;5(3):233–244. doi: 10.1016/s0174-173x(85)80013-3. [DOI] [PubMed] [Google Scholar]
  3. Clayman M. D., Martinez-Hernandez A., Michaud L., Alper R., Mann R., Kefalides N. A., Neilson E. G. Isolation and characterization of the nephritogenic antigen producing anti-tubular basement membrane disease. J Exp Med. 1985 Feb 1;161(2):290–305. doi: 10.1084/jem.161.2.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dehm P., Kefalides N. A. The collagenous component of lens basement membrane. The isolation and characterization of an alpha chain size collagenous peptide and its relationship to newly synthesized lens components. J Biol Chem. 1978 Oct 10;253(19):6680–6686. [PubMed] [Google Scholar]
  5. Dixit S. N. Isolation and characterization of two collagenous components from anterior lens capsule. FEBS Lett. 1978 Jan 1;85(1):153–157. doi: 10.1016/0014-5793(78)81269-1. [DOI] [PubMed] [Google Scholar]
  6. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  7. Engvall E., Ruoslahti E. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen. Int J Cancer. 1977 Jul 15;20(1):1–5. doi: 10.1002/ijc.2910200102. [DOI] [PubMed] [Google Scholar]
  8. Fessler L. I., Fessler J. H. Identification of the carboxyl peptides of mouse procollagen IV and its implications for the assembly and structure of basement membrane procollagen. J Biol Chem. 1982 Aug 25;257(16):9804–9810. [PubMed] [Google Scholar]
  9. Fillit H. M., Zabriskie J. B. Cellular immunity in glomerulonephritis. Am J Pathol. 1982 Nov;109(2):227–243. [PMC free article] [PubMed] [Google Scholar]
  10. Fillit H., Damle S. P., Gregory J. D., Volin C., Poon-King T., Zabriskie J. Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan. J Exp Med. 1985 Feb 1;161(2):277–289. doi: 10.1084/jem.161.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gay S., Miller E. J. Characterization of lens capsule collagen: evidence for the presence of two unique chains in molecules derived from major basement membrane structures. Arch Biochem Biophys. 1979 Dec;198(2):370–378. doi: 10.1016/0003-9861(79)90509-5. [DOI] [PubMed] [Google Scholar]
  12. Kefalides N. A. A collagen of unusual composition and a glycoprotein isolated from canine glomerular basement membrane. Biochem Biophys Res Commun. 1966 Jan 4;22(1):26–32. doi: 10.1016/0006-291x(66)90597-3. [DOI] [PubMed] [Google Scholar]
  13. Kefalides N. A., Alper R., Clark C. C. Biochemistry and metabolism of basement membranes. Int Rev Cytol. 1979;61:167–228. doi: 10.1016/s0074-7696(08)61998-1. [DOI] [PubMed] [Google Scholar]
  14. Kefalides N. A., Denduchis B. Structural components of epithelial and endothelial basement membranes. Biochemistry. 1969 Nov;8(11):4613–4621. doi: 10.1021/bi00839a057. [DOI] [PubMed] [Google Scholar]
  15. Kefalides N. A. Isolation and characterization of the collagen from glomerular basement membrane. Biochemistry. 1968 Sep;7(9):3103–3112. doi: 10.1021/bi00849a012. [DOI] [PubMed] [Google Scholar]
  16. Kefalides N. A. Isolation of a collagen from basement membranes containing three identical - chains. Biochem Biophys Res Commun. 1971 Oct 1;45(1):226–234. doi: 10.1016/0006-291x(71)90073-8. [DOI] [PubMed] [Google Scholar]
  17. Kresina T. F., Miller E. J. Isolation and characterization of basement membrane collagen from human placental tissue. Evidence for the presence of two genetically distinct collagen chains. Biochemistry. 1979 Jul 10;18(14):3089–3097. doi: 10.1021/bi00581a028. [DOI] [PubMed] [Google Scholar]
  18. Kühn K., Wiedemann H., Timpl R., Risteli J., Dieringer H., Voss T., Glanville R. W. Macromolecular structure of basement membrane collagens. FEBS Lett. 1981 Mar 9;125(1):123–128. doi: 10.1016/0014-5793(81)81012-5. [DOI] [PubMed] [Google Scholar]
  19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  20. Ohno M., Martinez-Hernandez A., Ohno N., Kefalides N. A. Isolation of laminin from human placental basement membranes: amnion, chorion and chorionic microvessels. Biochem Biophys Res Commun. 1983 May 16;112(3):1091–1098. doi: 10.1016/0006-291x(83)91730-8. [DOI] [PubMed] [Google Scholar]
  21. Olsen B. R., Alper R., Kefalides N. A. Structural characterization of a soluble fraction from lens-capsule basement membrane. Eur J Biochem. 1973 Oct 5;38(2):220–228. doi: 10.1111/j.1432-1033.1973.tb03053.x. [DOI] [PubMed] [Google Scholar]
  22. Phillips G. N., Jr, Flicker P. F., Cohen C., Manjula B. N., Fischetti V. A. Streptococcal M protein: alpha-helical coiled-coil structure and arrangement on the cell surface. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4689–4693. doi: 10.1073/pnas.78.8.4689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tanzer M. L., Kefalides N. A. Collagen crosslinks: occurrence in basement membrane collagens. Biochem Biophys Res Commun. 1973 Apr 2;51(3):775–780. doi: 10.1016/0006-291x(73)91382-x. [DOI] [PubMed] [Google Scholar]
  24. Timpl R., Martin G. R., Bruckner P., Wick G., Wiedemann H. Nature of the collagenous protein in a tumor basement membrane. Eur J Biochem. 1978 Mar;84(1):43–52. doi: 10.1111/j.1432-1033.1978.tb12139.x. [DOI] [PubMed] [Google Scholar]
  25. Timpl R., Rohde H., Robey P. G., Rennard S. I., Foidart J. M., Martin G. R. Laminin--a glycoprotein from basement membranes. J Biol Chem. 1979 Oct 10;254(19):9933–9937. [PubMed] [Google Scholar]
  26. Timpl R., Wiedemann H., van Delden V., Furthmayr H., Kühn K. A network model for the organization of type IV collagen molecules in basement membranes. Eur J Biochem. 1981 Nov;120(2):203–211. doi: 10.1111/j.1432-1033.1981.tb05690.x. [DOI] [PubMed] [Google Scholar]
  27. Wieslander J., Barr J. F., Butkowski R. J., Edwards S. J., Bygren P., Heinegård D., Hudson B. G. Goodpasture antigen of the glomerular basement membrane: localization to noncollagenous regions of type IV collagen. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3838–3842. doi: 10.1073/pnas.81.12.3838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wieslander J., Langeveld J., Butkowski R., Jodlowski M., Noelken M., Hudson B. G. Physical and immunochemical studies of the globular domain of type IV collagen. Cryptic properties of the Goodpasture antigen. J Biol Chem. 1985 Jul 15;260(14):8564–8570. [PubMed] [Google Scholar]
  29. Zabriskie J. B., Utermohlen V., Read S. E., Fischetti V. A. Streptococcus-related glomerulonephritis. Kidney Int. 1973 Feb;3(2):100–104. doi: 10.1038/ki.1973.16. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES