Abstract
A hybridoma antibody (11E7-1) was isolated from a myeloma fusion with nu/nu BALB/c immunized against the T15 idiotype. This IgM antibody exhibited a dual specificity, binding both to PC and to anti-PC antibodies from two idiotype families. Binding to PC and anti-PC antibodies are completely inhibited by PC analogs. Furthermore, the hybridoma antibody binds to itself. Self-binding is also inhibited by PC analogs. From these data, we suggest that 11E7-1 hybridoma antibody has a PC-specific paratope site, and at same time expresses the internal PC antigen idiotope. The term autobody is proposed to signify its self-binding and potential role in autoimmunity. Autobodies may have a unique role in the network of immune system. Furthermore, it may be a model for designing idiotype vaccines.
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Selected References
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- Berzofsky J. A. Intrinsic and extrinsic factors in protein antigenic structure. Science. 1985 Sep 6;229(4717):932–940. doi: 10.1126/science.2410982. [DOI] [PubMed] [Google Scholar]
- Bona C. A., Finley S., Waters S., Kunkel H. G. Anti-immunoglobulin antibodies. III. Properties of sequential anti-idiotypic antibodies to heterologous anti-gamma globulins. Detection of reactivity of anti-idiotype antibodies with epitopes of Fc fragments (homobodies) and with epitopes and idiotopes (epibodies). J Exp Med. 1982 Oct 1;156(4):986–999. doi: 10.1084/jem.156.4.986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Briles D. E., Nahm M., Schroer K., Davie J., Baker P., Kearney J., Barletta R. Antiphosphocholine antibodies found in normal mouse serum are protective against intravenous infection with type 3 streptococcus pneumoniae. J Exp Med. 1981 Mar 1;153(3):694–705. doi: 10.1084/jem.153.3.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chen P. P., Fong S., Houghten R. A., Carson D. A. Characterization of an epibody. An antiidiotype that reacts with both the idiotype of rheumatoid factors (RF) and the antigen recognized by RF. J Exp Med. 1985 Feb 1;161(2):323–331. doi: 10.1084/jem.161.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jerne N. K., Roland J., Cazenave P. A. Recurrent idiotopes and internal images. EMBO J. 1982;1(2):243–247. doi: 10.1002/j.1460-2075.1982.tb01154.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
- Kieber-Emmons T., Ward R. E., Raychaudhuri S., Rein R., Kohler H. Rational design and application of idiotope vaccines. Int Rev Immunol. 1986 Jan;1(1):1–26. doi: 10.3109/08830188609056598. [DOI] [PubMed] [Google Scholar]
- Kohler H., McNamara M., Ward R. E. Idiotypes as internal antigens. Prog Clin Biol Res. 1985;172B:343–353. [PubMed] [Google Scholar]
- Kohler H., Muller S., Bona C. Internal antigen and immune network. Proc Soc Exp Biol Med. 1985 Feb;178(2):189–195. doi: 10.3181/00379727-178-41996a. [DOI] [PubMed] [Google Scholar]
- Leon M. A., Young N. M. Specificity for phosphorylcholine of six murine myeloma proteins reactive with Pneumococcus C polysaccharide and beta-lipoprotein. Biochemistry. 1971 Apr 13;10(8):1424–1429. doi: 10.1021/bi00784a024. [DOI] [PubMed] [Google Scholar]
- Lindenmann J. Speculations on idiotypes and homobodies. Ann Immunol (Paris) 1973 May;124(2):171–184. [PubMed] [Google Scholar]
- McNamara M., Gleason K., Kohler H. T-cell helper circuits. Immunol Rev. 1984 Jun;79:87–102. doi: 10.1111/j.1600-065x.1984.tb00488.x. [DOI] [PubMed] [Google Scholar]
- McNamara M., Kang C. Y., Kohler H. Analysis of a TH1----TH2 helper cell circuit. J Immunol. 1985 Sep;135(3):1603–1609. [PubMed] [Google Scholar]
- Rowley D. A., Köhler H., Cowan J. D. An immunologic network. Contemp Top Immunobiol. 1980;9:205–230. doi: 10.1007/978-1-4615-9131-3_8. [DOI] [PubMed] [Google Scholar]
- Segal D. M., Padlan E. A., Cohen G. H., Rudikoff S., Potter M., Davies D. R. The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4298–4302. doi: 10.1073/pnas.71.11.4298. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wittner M. K., Bach M. A., Köhler H. Immune response to phosphorylcholine. IX. Characterization of hybridoma anti-TEPC15 antibodies. J Immunol. 1982 Feb;128(2):595–599. [PubMed] [Google Scholar]
- Wu T. T., Kabat E. A. An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity. J Exp Med. 1970 Aug 1;132(2):211–250. doi: 10.1084/jem.132.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]