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. 1986 Jul 1;164(1):72–89. doi: 10.1084/jem.164.1.72

Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages

PMCID: PMC2188189  PMID: 2425032

Abstract

An IgM mAb (BB10) was produced by immunization of mice with human eosinophils purified according to their abnormal low density ("hypodense" cells), and previously shown to exhibit increased IgE- dependent antiparasite cytotoxicity. This BB10 antibody, selected for positive fluorescence staining of hypodense blood or lung eosinophils and low or negative staining of normodense eosinophils or neutrophils, could strongly inhibit IgE-dependent cytotoxicity of human eosinophils and platelets. The specificity for the IgE Fc receptor was suggested by the high levels of inhibition of IgE rosettes formed by eosinophils after incubation with the purified IgM fraction of BB10, whereas other receptors (Fc gamma R, CR1) were not affected. On the other hand, BB10, able to inhibit rat eosinophil Fc epsilon R, did not react with the IgE Fc receptor on mast cells or basophils. A technique using radioiodinated BB10 allowed us to quantify the specific binding of BB10 to human eosinophils and platelets. Competition experiments revealed a crossinhibition between the binding of BB10 and IgE, suggesting the specificity of BB10 for the IgE binding site of eosinophil, platelet, and monocyte Fc epsilon R. Three proteins having extrapolated Mr of 32,000, 43,000-45,000, and 97,000 were found in the platelet extract eluted from a BB10 or from an IgE immunosorbent column. These findings confirm the similarities between IgE Fc receptors on human eosinophils, platelets, and macrophages, already observed with polyclonal antibodies directed against the B lymphocyte Fc epsilon receptor. They suggest, moreover, that the mAb BB10 can represent a good reagent for further investigations on the structure and the functions of this IgE Fc receptor (Fc epsilon R2).

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Selected References

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  1. Barsumian E. L., Isersky C., Petrino M. G., Siraganian R. P. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur J Immunol. 1981 Apr;11(4):317–323. doi: 10.1002/eji.1830110410. [DOI] [PubMed] [Google Scholar]
  2. Capron A., Ameisen J. C., Joseph M., Auriault C., Tonnel A. B., Caen J. New functions for platelets and their pathological implications. Int Arch Allergy Appl Immunol. 1985;77(1-2):107–114. doi: 10.1159/000233762. [DOI] [PubMed] [Google Scholar]
  3. Capron A., Capron M., Dupas H., Bout D., Petitprez A. Etude in vitro des phénomènes immunologiques dans la schistosomiase humaine et expérimentale. I. Etude comparative in vitro de l'activité léthale d'immunsérums sur les formes immatures et sur les adultes de Schistosoma mansoni. Int J Parasitol. 1974 Dec;4(6):613–623. doi: 10.1016/0020-7519(74)90026-5. [DOI] [PubMed] [Google Scholar]
  4. Capron M., Kusnierz J. P., Prin L., Spiegelberg H. L., Ovlaque G., Gosset P., Tonnel A. B., Capron A. Cytophilic IgE on human blood and tissue eosinophils: detection by flow microfluorometry. J Immunol. 1985 May;134(5):3013–3018. [PubMed] [Google Scholar]
  5. Capron M., Nogueira-Queiroz J. A., Papin J. P., Capron A. Interactions between eosinophils and antibodies: in vivo protective role against rat schistosomiasis. Cell Immunol. 1984 Jan;83(1):60–72. doi: 10.1016/0008-8749(84)90225-9. [DOI] [PubMed] [Google Scholar]
  6. Capron M., Spiegelberg H. L., Prin L., Bennich H., Butterworth A. E., Pierce R. J., Ouaissi M. A., Capron A. Role of IgE receptors in effector function of human eosinophils. J Immunol. 1984 Jan;132(1):462–468. [PubMed] [Google Scholar]
  7. Davis W. B., Fells G. A., Sun X. H., Gadek J. E., Venet A., Crystal R. G. Eosinophil-mediated injury to lung parenchymal cells and interstitial matrix. A possible role for eosinophils in chronic inflammatory disorders of the lower respiratory tract. J Clin Invest. 1984 Jul;74(1):269–278. doi: 10.1172/JCI111411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Finbloom D. S., Metzger H. Binding of immunoglobulin E to the receptor on rat peritoneal macrophages. J Immunol. 1982 Nov;129(5):2004–2008. [PubMed] [Google Scholar]
  9. Finbloom D. S., Metzger H. Isolation of cross-linked IgE-receptor complexes from rat macrophages. J Immunol. 1983 Apr;130(4):1489–1491. [PubMed] [Google Scholar]
  10. Fischer E., Kazatchkine M. D. Surface-dependent modulation by H of C5 cleavage by the cell-bound alternative pathway C5 convertase of human complement. J Immunol. 1983 Jun;130(6):2821–2824. [PubMed] [Google Scholar]
  11. Foon K. A., Buescher S., Kimball E. S., Huang L. C., Stevenson H. C., Clarke G., Gregorio T., Harley J. B. Monoclonal antibody to human eosinophils recognizing 95 kD surface membrane antigen. Hybridoma. 1983;2(4):393–402. doi: 10.1089/hyb.1983.2.393. [DOI] [PubMed] [Google Scholar]
  12. Joseph M., Ameisen J. C., Kusnierz J. P., Pancré V., Capron M., Capron A. Participation du récepteur pour l'IgE à la toxicité des plaquettes sanguines contre les schistosomes. C R Acad Sci III. 1984;298(3):55–60. [PubMed] [Google Scholar]
  13. Joseph M., Auriault C., Capron M., Ameisen J. C., Pancré V., Torpier G., Kusnierz J. P., Ovlaque G., Capron A. IgE-dependent platelet cytotoxicity against helminths. Adv Exp Med Biol. 1985;184:23–33. doi: 10.1007/978-1-4684-8326-0_2. [DOI] [PubMed] [Google Scholar]
  14. Joseph M., Tonnel A. B., Capron A., Voisin C. Enzyme release and superoxide anion production by human alveolar macrophages stimulated with immunoglobulin E. Clin Exp Immunol. 1980 May;40(2):416–422. [PMC free article] [PubMed] [Google Scholar]
  15. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  16. Meinke G. C., Magro A. M., Lawrence D. A., Spiegelberg H. L. Characterization of an IgE receptor isolated from cultured B-type lymphoblastoid cells. J Immunol. 1978 Oct;121(4):1321–1328. [PubMed] [Google Scholar]
  17. Melewicz F. M., Plummer J. M., Spiegelberg H. L. Comparison of the Fc receptors for IgE on human lymphocytes and monocytes. J Immunol. 1982 Aug;129(2):563–569. [PubMed] [Google Scholar]
  18. Morich F. J., Momburg F., Moldenhauer G., Hartmann K. U., Bross K. J. Immunoperoxidase slide assay (IPSA)--a new screening method for hybridoma supernatants directed against cell surface antigens compared to other binding assays. Immunobiology. 1983 Mar;164(2):192–202. doi: 10.1016/S0171-2985(83)80010-2. [DOI] [PubMed] [Google Scholar]
  19. Patscheke H. Shape and functional properties of human platelets washed with acid citrate. Haemostasis. 1981;10(1):14–27. doi: 10.1159/000214383. [DOI] [PubMed] [Google Scholar]
  20. Prin L., Capron M., Gosset P., Wallaert B., Kusnierz J. P., Bletry O., Tonnel A. B., Capron A. Eosinophilic lung disease: immunological studies of blood and alveolar eosinophils. Clin Exp Immunol. 1986 Jan;63(1):249–257. [PMC free article] [PubMed] [Google Scholar]
  21. Prin L., Capron M., Tonnel A. B., Bletry O., Capron A. Heterogeneity of human peripheral blood eosinophils: variability in cell density and cytotoxic ability in relation to the level and the origin of hypereosinophilia. Int Arch Allergy Appl Immunol. 1983;72(4):336–346. doi: 10.1159/000234893. [DOI] [PubMed] [Google Scholar]
  22. Prin L., Charon J., Capron M., Gosset P., Taelman H., Tonnel A. B., Capron A. Heterogeneity of human eosinophils. II. Variability of respiratory burst activity related to cell density. Clin Exp Immunol. 1984 Sep;57(3):735–742. [PMC free article] [PubMed] [Google Scholar]
  23. Rector E., Nakajima T., Rocha C., Duncan D., Lestourgeon D., Mitchell R. S., Fischer J., Sehon A. H., Delespesse G. Detection and characterization of monoclonal antibodies specific to IgE receptors on human lymphocytes by flow cytometry. Immunology. 1985 Jul;55(3):481–488. [PMC free article] [PubMed] [Google Scholar]
  24. Tai P. C., Bakes D. M., Barkans J. R., Spry C. J. Plasma membrane antigens on light density and activated human blood eosinophils. Clin Exp Immunol. 1985 May;60(2):427–436. [PMC free article] [PubMed] [Google Scholar]
  25. Vadas M. A., David J. R., Butterworth A., Pisani N. T., Siongok T. A. A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. J Immunol. 1979 Apr;122(4):1228–1236. [PubMed] [Google Scholar]
  26. Winqvist I., Olofsson T., Olsson I., Persson A. M., Hallberg T. Altered density, metabolism and surface receptors of eosinophils in eosinophilia. Immunology. 1982 Nov;47(3):531–539. [PMC free article] [PubMed] [Google Scholar]

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