Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1987 Jan 1;165(1):87–96. doi: 10.1084/jem.165.1.87

Epitopes associated with the MHC restriction site of T cells. II. Somatic generation of Iat epitopes on T cells in radiation bone marrow chimeras

PMCID: PMC2188263  PMID: 2432153

Abstract

We described in this paper systematic alterations in the expression of unique I region controlled epitopes on helper T cells (Th) in chimeras according to the changes in their H-2 restriction specificity. Taking advantage of the reactivity of monoclonal antibodies (anti-Iat) putatively specific for the epitopes indirectly controlled by I region and expressed in association with the Iak restriction site of Th, we examined the alterations of these epitopes on Th cells from various bone marrow chimeras. Iatk epitopes were physiologically expressed on Iak-restricted but not on Iab-restricted Th cells in (H-2k X H-2b)F1 mice. In the chimeric condition, the H-2k-restricted Th of B6----F1 chimera acquired the expression of Iatk even though B6 Th is unable to express Iatk when developed under the physiologic condition. Iatk are also found on Th of fully allogeneic chimera of B6----C3H, whereas Th cells of C3H----B6 completely lost the Iatk expression. These results indicate that Iat epitopes originally defined as unique I region- controlled determinants selectively expressed on T cells are not encoded by the I region genes but are associated with the T cell receptor that sees the self Ia. The epitopes undergo the adaptive alterations according to the acquisition of a new MHC restriction. This is the first example to demonstrate the epitope associated with T cell receptor which undergo the systematic adaptive differentiation.

Full Text

The Full Text of this article is available as a PDF (592.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison J. P., Ridge L., Lund J., Gross-Pelose J., Lanier L., McIntyre B. W. The murine T cell antigen receptor and associated structures. Immunol Rev. 1984 Oct;81:145–160. doi: 10.1111/j.1600-065x.1984.tb01108.x. [DOI] [PubMed] [Google Scholar]
  2. Asano Y., Hodes R. J. T cell regulation of B cell activation. I-A-restricted T suppressor cells inhibit the major histocompatibility complex-restricted interactions of T helper cells with B cells and accessory cells. J Exp Med. 1983 Jun 1;157(6):1867–1884. doi: 10.1084/jem.157.6.1867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Asano Y., Singer A., Hodes R. J. Role of the major histocompatibility complex in T cell activation of B cell subpopulations. Major histocompatibility complex-restricted and -unrestricted B cell responses are mediated by distinct B cell subpopulations. J Exp Med. 1981 Oct 1;154(4):1100–1115. doi: 10.1084/jem.154.4.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bevan M. J., Fink P. J. The influence of thymus H-2 antigens on the specificity of maturing killer and helper cells. Immunol Rev. 1978;42:3–19. doi: 10.1111/j.1600-065x.1978.tb00256.x. [DOI] [PubMed] [Google Scholar]
  5. Cantor H., Gershon R. K. Immunological circuits: cellular composition. Fed Proc. 1979 Jun;38(7):2058–2064. [PubMed] [Google Scholar]
  6. Davis M. M., Chien Y. H., Gascoigne N. R., Hedrick S. M. A murine T cell receptor gene complex: isolation, structure and rearrangement. Immunol Rev. 1984 Oct;81:235–258. doi: 10.1111/j.1600-065x.1984.tb01113.x. [DOI] [PubMed] [Google Scholar]
  7. Dembić Z., Haas W., Weiss S., McCubrey J., Kiefer H., von Boehmer H., Steinmetz M. Transfer of specificity by murine alpha and beta T-cell receptor genes. Nature. 1986 Mar 20;320(6059):232–238. doi: 10.1038/320232a0. [DOI] [PubMed] [Google Scholar]
  8. Dialynas D. P., Wilde D. B., Marrack P., Pierres A., Wall K. A., Havran W., Otten G., Loken M. R., Pierres M., Kappler J. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29–56. doi: 10.1111/j.1600-065x.1983.tb01083.x. [DOI] [PubMed] [Google Scholar]
  9. Haskins K., Kappler J., Marrack P. The major histocompatibility complex-restricted antigen receptor on T cells. Annu Rev Immunol. 1984;2:51–66. doi: 10.1146/annurev.iy.02.040184.000411. [DOI] [PubMed] [Google Scholar]
  10. Hiramatsu K., Miyatani S., Kim M., Yamada S., Okumura K., Tada T. Unique T cell Ia antigen expressed on a hybrid cell line producing antigen-specific augmenting T cell factor. J Immunol. 1981 Sep;127(3):1118–1122. [PubMed] [Google Scholar]
  11. Hiramatsu K., Ochi A., Miyatani S., Segawa A., Tada T. Monoclonal antibodies against unique I-region gene products expressed only on mature functional T cells. Nature. 1982 Apr 15;296(5858):666–668. doi: 10.1038/296666a0. [DOI] [PubMed] [Google Scholar]
  12. Katz D. H. The role of the histocompatibilty gene complex in lymphocyte defferetiation. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):611–624. doi: 10.1101/sqb.1977.041.01.070. [DOI] [PubMed] [Google Scholar]
  13. Mak T. W., Yanagi Y. Genes encoding the human T cell antigen receptor. Immunol Rev. 1984 Oct;81:221–233. doi: 10.1111/j.1600-065x.1984.tb01112.x. [DOI] [PubMed] [Google Scholar]
  14. Meuer S. C., Acuto O., Hercend T., Schlossman S. F., Reinherz E. L. The human T-cell receptor. Annu Rev Immunol. 1984;2:23–50. doi: 10.1146/annurev.iy.02.040184.000323. [DOI] [PubMed] [Google Scholar]
  15. Miyatani S., Hiramatsu K., Nakajima P. B., Owen F. L., Tada T. Structural analysis of antigen-specific Ia-bearing regulatory T-cell factors: gel electrophoretic analysis of the antigen-specific augmenting T -cell factor. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6336–6340. doi: 10.1073/pnas.80.20.6336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nakajima P. B., Ochi A., Owen F. L., Tada T. Presence of IgT-C and I-A subregion-encoded determinants on distinct chains of monoclonal antigen-specific augmenting factor derived from a T cell hybridoma. J Exp Med. 1983 Jun 1;157(6):2110–2120. doi: 10.1084/jem.157.6.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  18. Samelson L. E., Schwartz R. H. Characterization of the antigen-specific T cell receptor from a pigeon cytochrome c-specific T cell hybrid. Immunol Rev. 1984 Oct;81:131–144. doi: 10.1111/j.1600-065x.1984.tb01107.x. [DOI] [PubMed] [Google Scholar]
  19. Schwartz R. H. T-lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex. Annu Rev Immunol. 1985;3:237–261. doi: 10.1146/annurev.iy.03.040185.001321. [DOI] [PubMed] [Google Scholar]
  20. Singer A., Hathcock K. S., Hodes R. J. Cellular and genetic control of antibody responses. V. Helper T-cell recognition of H-2 determinants on accessory cells but not B cells. J Exp Med. 1979 May 1;149(5):1208–1226. doi: 10.1084/jem.149.5.1208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Singer A., Hodes R. J. Mechanisms of T cell-B cell interaction. Annu Rev Immunol. 1983;1:211–241. doi: 10.1146/annurev.iy.01.040183.001235. [DOI] [PubMed] [Google Scholar]
  22. Sprent J. Role of H-2 gene products in the function of T helper cells from normal and chimeric mice in vivo. Immunol Rev. 1978;42:108–137. doi: 10.1111/j.1600-065x.1978.tb00260.x. [DOI] [PubMed] [Google Scholar]
  23. Tokuhisa T., Taniguchi M., Okumura K., Tada T. An antigen-specific I region gene product that augments the antibody response. J Immunol. 1978 Feb;120(2):414–421. [PubMed] [Google Scholar]
  24. Uracz W., Asano Y., Abe R., Tada T. I-J epitopes are adaptively acquired by T cells differentiated in the chimaeric condition. Nature. 1985 Aug 22;316(6030):741–743. doi: 10.1038/316741a0. [DOI] [PubMed] [Google Scholar]
  25. Yagi J., Nonaka M., Abe R., Tada T. Ir gene expression on T cells: effect of a monoclonal antibody directed against I region-controlled determinants on T cells. Eur J Immunol. 1986 May;16(5):497–503. doi: 10.1002/eji.1830160506. [DOI] [PubMed] [Google Scholar]
  26. Zinkernagel R. M., Doherty P. C. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979;27:51–177. doi: 10.1016/s0065-2776(08)60262-x. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES