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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1987 Mar 1;165(3):920–925. doi: 10.1084/jem.165.3.920

Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc

PMCID: PMC2188273  PMID: 3493323

Abstract

The monoclonal antibody 6C3 was used to test a wide variety of murine hematopoietic neoplasms for cell surface expression of a 160 kD glycoprotein (gp160(6C3)) previously shown to be expressed by neoplastic pre-B and some B lymphocytes transformed by Abelson murine leukemia virus (A-MuLV). This antigen was expressed on many pre-B and B cell lymphomas, but not on A-MuLV-transformed fibroblasts, T cell lymphomas, or myelomonocytic leukemias, gp160(6C3) was expressed by most early B-lineage spontaneous tumors, and early B tumors induced by replication-defective MuLV-containing oncogenes the products of which are associated with the cytoplasmic aspect of the plasma membrane, i.e., fes, abl, H-ras, bas, src, erbB, and Cas NS-1. By comparison, none of the early B lineage lymphomas induced by the "nuclear" oncogene avian v-myc MuLV, or arising in mice transgenic for a murine c-myc gene, or later B cell lineage stages bearing translocations of the c- myc locus expressed this antigen.

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Selected References

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  1. Adams J. M., Harris A. W., Langdon W. Y., Pinkert C. A., Brinster R. L., Palmiter R. D., Corcoran L., Alexander W. S., Graham M. W., Cory S. c-myc-induced lymphomagenesis in transgenic mice and the role of the Pvt-1 locus in lymphoid neoplasia. Curr Top Microbiol Immunol. 1986;132:1–8. doi: 10.1007/978-3-642-71562-4_1. [DOI] [PubMed] [Google Scholar]
  2. Angel J. M., Bedigian H. G. Expression of murine leukemia viruses in B-cell lymphomas of CWD/Agl mice. J Virol. 1984 Nov;52(2):691–694. doi: 10.1128/jvi.52.2.691-694.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bauer S. R., Holmes K. L., Morse H. C., 3rd, Potter M. Clonal relationship of the lymphoblastic cell line P388 to the macrophage cell line P388D1 as evidenced by immunoglobulin gene rearrangements and expression of cell surface antigens. J Immunol. 1986 Jun 15;136(12):4695–4699. [PubMed] [Google Scholar]
  4. Fredrickson T. N., Morse H. C., 3rd, Rowe W. P. Spontaneous tumors of NFS mice congenic for ecotropic murine leukemia virus induction loci. J Natl Cancer Inst. 1984 Aug;73(2):521–524. doi: 10.1093/jnci/73.2.521. [DOI] [PubMed] [Google Scholar]
  5. Holmes K. L., Langdon W. Y., Fredrickson T. N., Coffman R. L., Hoffman P. M., Hartley J. W., Morse H. C., 3rd Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts. J Immunol. 1986 Jul 15;137(2):679–688. [PubMed] [Google Scholar]
  6. Holmes K. L., Palaszynski E., Fredrickson T. N., Morse H. C., 3rd, Ihle J. N. Correlation of cell-surface phenotype with the establishment of interleukin 3-dependent cell lines from wild-mouse murine leukemia virus-induced neoplasms. Proc Natl Acad Sci U S A. 1985 Oct;82(19):6687–6691. doi: 10.1073/pnas.82.19.6687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes K. L., Pierce J. H., Davidson W. F., Morse H. C., 3rd Murine hematopoietic cells with pre-B or pre-B/myeloid characteristics are generated by in vitro transformation with retroviruses containing fes, ras, abl, and src oncogenes. J Exp Med. 1986 Aug 1;164(2):443–457. doi: 10.1084/jem.164.2.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klein G., Klein E. Evolution of tumours and the impact of molecular oncology. Nature. 1985 May 16;315(6016):190–195. doi: 10.1038/315190a0. [DOI] [PubMed] [Google Scholar]
  9. Land H., Parada L. F., Weinberg R. A. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature. 1983 Aug 18;304(5927):596–602. doi: 10.1038/304596a0. [DOI] [PubMed] [Google Scholar]
  10. Lanford R. E., Butel J. S. Intracellular transport of SV40 large tumor antigen: a mutation which abolishes migration to the nucleus does not prevent association with the cell surface. Virology. 1982 May;119(1):169–184. doi: 10.1016/0042-6822(82)90074-5. [DOI] [PubMed] [Google Scholar]
  11. Morse H. C., 3rd, Hartley J. W., Fredrickson T. N., Yetter R. A., Cleveland J. L., Majumdar C., Rapp U. R. Tumors of newborn NFS/N mice infected with murine retroviruses containing avian v-myc. Curr Top Microbiol Immunol. 1986;132:17–22. doi: 10.1007/978-3-642-71562-4_3. [DOI] [PubMed] [Google Scholar]
  12. Morse H. C., 3rd, Yetter R. A., Stimpfling J. H., Pitts O. M., Fredrickson T. N., Hartley J. W. Greying with age in mice: relation to expression of murine leukemia viruses. Cell. 1985 Jun;41(2):439–448. doi: 10.1016/s0092-8674(85)80017-9. [DOI] [PubMed] [Google Scholar]
  13. Pierce J. H., Gazit A., Di Fiore P. P., Kraus M., Pennington C. Y., Holmes K. L., Davidson W. F., Morse H. C., 3rd, Aaronson S. A. Mammalian cell transformation by a recombinant murine retrovirus containing the avian erythroblastosis virus erbB gene. Curr Top Microbiol Immunol. 1986;132:55–61. doi: 10.1007/978-3-642-71562-4_8. [DOI] [PubMed] [Google Scholar]
  14. Pillemer E., Whitlock C., Weissman I. L. Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4434–4438. doi: 10.1073/pnas.81.14.4434. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shen-Ong G. L., Keath E. J., Piccoli S. P., Cole M. D. Novel myc oncogene RNA from abortive immunoglobulin-gene recombination in mouse plasmacytomas. Cell. 1982 Dec;31(2 Pt 1):443–452. doi: 10.1016/0092-8674(82)90137-4. [DOI] [PubMed] [Google Scholar]
  16. Tidmarsh G. F., Dailey M. O., Whitlock C. A., Pillemer E., Weissman I. L. Transformed lymphocytes from Abelson-diseased mice express levels of a B lineage transformation-associated antigen elevated from that found on normal lymphocytes. J Exp Med. 1985 Nov 1;162(5):1421–1434. doi: 10.1084/jem.162.5.1421. [DOI] [PMC free article] [PubMed] [Google Scholar]

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