Abstract
Plasmodium falciparum malaria merozoites require erythrocyte sialic acid for optimal invasion of human erythrocytes. Since mouse erythrocytes have the form of sialic acid found on human erythrocytes (N-acetyl neuraminic acid), mouse erythrocytes were tested for invasion in vitro. The Camp and 7G8 strains of P. falciparum invaded mouse erythrocytes at 17-45% of the invasion rate of human erythrocytes. Newly invaded mouse erythrocytes morphologically resembled parasitized human erythrocytes as shown on Giemsa-stained blood films and by electron microscopy. The rim of parasitized mouse erythrocytes contained the P. falciparum 155-kD protein, which is on the rim of ring- infected human erythrocytes. Camp but not 7G8 invaded rat erythrocytes, indicating receptor heterogeneity. These data suggest that it may be possible to adapt the asexual erythrocytic stage of P. falciparum to rodents. The development of a rodent model of P. falciparum malaria could facilitate vaccine development.
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Selected References
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- Aley S. B., Sherwood J. A., Howard R. J. Knob-positive and knob-negative Plasmodium falciparum differ in expression of a strain-specific malarial antigen on the surface of infected erythrocytes. J Exp Med. 1984 Nov 1;160(5):1585–1590. doi: 10.1084/jem.160.5.1585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown G. V., Culvenor J. G., Crewther P. E., Bianco A. E., Coppel R. L., Saint R. B., Stahl H. D., Kemp D. J., Anders R. F. Localization of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum in merozoites and ring-infected erythrocytes. J Exp Med. 1985 Aug 1;162(2):774–779. doi: 10.1084/jem.162.2.774. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burkot T. R., Williams J. L., Schneider I. Infectivity to mosquitoes of Plasmodium falciparum clones grown in vitro from the same isolate. Trans R Soc Trop Med Hyg. 1984;78(3):339–341. doi: 10.1016/0035-9203(84)90114-7. [DOI] [PubMed] [Google Scholar]
- Hadley T. J., Erkmen Z., Kaufman B. M., Futrovsky S., McGuinnis M. H., Graves P., Sadoff J. C., Miller L. H. Factors influencing invasion of erythrocytes by Plasmodium falciparum parasites: the effects of an N-acetyl glucosamine neoglycoprotein and an anti-glycophorin A antibody. Am J Trop Med Hyg. 1986 Sep;35(5):898–905. doi: 10.4269/ajtmh.1986.35.898. [DOI] [PubMed] [Google Scholar]
- Hadley T. J. Invasion of erythrocytes by malaria parasites: a cellular and molecular overview. Annu Rev Microbiol. 1986;40:451–477. doi: 10.1146/annurev.mi.40.100186.002315. [DOI] [PubMed] [Google Scholar]
- Haynes J. D., Diggs C. L., Hines F. A., Desjardins R. E. Culture of human malaria parasites Plasmodium falciparum. Nature. 1976 Oct 28;263(5580):767–769. doi: 10.1038/263767a0. [DOI] [PubMed] [Google Scholar]
- Johnson G. D., Nogueira Araujo G. M. A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Methods. 1981;43(3):349–350. doi: 10.1016/0022-1759(81)90183-6. [DOI] [PubMed] [Google Scholar]
- MCGHEE R. B. The influence of age of the animal upon the susceptibility of mammalian erythrocytes to infection by the avian malaria parasite Plasmodium lophurae. J Infect Dis. 1953 Jan-Feb;92(1):4–9. doi: 10.1093/infdis/92.1.4. [DOI] [PubMed] [Google Scholar]
- Mason S. J., Miller L. H., Shiroishi T., Dvorak J. A., McGinniss M. H. The Duffy blood group determinants: their role in the susceptibility of human and animal erythrocytes to Plasmodium knowlesi malaria. Br J Haematol. 1977 Jul;36(3):327–335. doi: 10.1111/j.1365-2141.1977.tb00656.x. [DOI] [PubMed] [Google Scholar]
- McCutchan T. F., Dame J. B., Miller L. H., Barnwell J. Evolutionary relatedness of Plasmodium species as determined by the structure of DNA. Science. 1984 Aug 24;225(4664):808–811. doi: 10.1126/science.6382604. [DOI] [PubMed] [Google Scholar]
- Miller L. H., Aikawa M., Johnson J. G., Shiroishi T. Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation. J Exp Med. 1979 Jan 1;149(1):172–184. doi: 10.1084/jem.149.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchell G. H., Hadley T. J., McGinniss M. H., Klotz F. W., Miller L. H. Invasion of erythrocytes by Plasmodium falciparum malaria parasites: evidence for receptor heterogeneity and two receptors. Blood. 1986 May;67(5):1519–1521. [PubMed] [Google Scholar]
- Perlmann H., Berzins K., Wahlgren M., Carlsson J., Björkman A., Patarroyo M. E., Perlmann P. Antibodies in malarial sera to parasite antigens in the membrane of erythrocytes infected with early asexual stages of Plasmodium falciparum. J Exp Med. 1984 Jun 1;159(6):1686–1704. doi: 10.1084/jem.159.6.1686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reuter G., Vliegenthart J. F., Wember M., Schauer R., Howard R. J. Identification of 9-O-acetyl-N-acetylneuraminic acid on the surface of BALB/c mouse erythrocytes. Biochem Biophys Res Commun. 1980 May 30;94(2):567–572. doi: 10.1016/0006-291x(80)91269-3. [DOI] [PubMed] [Google Scholar]
- Trager W., Jensen J. B. Human malaria parasites in continuous culture. Science. 1976 Aug 20;193(4254):673–675. doi: 10.1126/science.781840. [DOI] [PubMed] [Google Scholar]
- Vernes A., Haynes J. D., Tapchaisri P., Williams J. L., Dutoit E., Diggs C. L. Plasmodium falciparum strain-specific human antibody inhibits merozoite invasion of erythrocytes. Am J Trop Med Hyg. 1984 Mar;33(2):197–203. doi: 10.4269/ajtmh.1984.33.197. [DOI] [PubMed] [Google Scholar]