Abstract
We used a cloned, TNP-specific, MHC-restricted, human Th cell line, E- 11, and an assay of cognate Th-B cell interaction, BLAST-2 antigen expression on the B cell surface, to investigate the functional nature of the Th cell antigen receptor. We observed that E-11 induces BLAST-2 expression by resting B cells in a hapten-dependent, hapten-specific, but MHC nonrestricted manner. The implication of these results for the Th cell receptor are discussed.
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- Blanden R. V., Gill D. B., Zidovetzki R., Pecht I. Cooperativity in antigen binding as a potential element in Tc cell recognition. Mol Immunol. 1980 Jul;17(7):893–896. doi: 10.1016/0161-5890(80)90037-1. [DOI] [PubMed] [Google Scholar]
- Carel S., Bron C., Corradin G. T-cell hybridoma specific for a cytochrome c peptide: specific antigen binding and interleukin 2 production. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4832–4836. doi: 10.1073/pnas.80.15.4832. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cleveland W. L., Erlanger B. F. Hypothesis: the MHC-restricted T-cell receptor as a structure with two multistate allosteric combining sites. Mol Immunol. 1984 Nov;21(11):1037–1046. doi: 10.1016/0161-5890(84)90113-5. [DOI] [PubMed] [Google Scholar]
- Crow M. K., Jover J. A., Friedman S. M. Direct T helper-B cell interactions induce an early B cell activation antigen. J Exp Med. 1986 Nov 1;164(5):1760–1772. doi: 10.1084/jem.164.5.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedman S. M., Thompson G. S., Principato M. A. Allospecific human T cell lines and clones which mediate HLA-DR restricted helper activity. J Immunol. 1982 Dec;129(6):2451–2457. [PubMed] [Google Scholar]
- Goldberg D., Green A., Gottlieb A. B., Crow M. K., Lewison A., Friedman S. M. Cloned allospecific human helper T cell lines induce an MHC-restricted proliferative response by resting B cells. J Immunol. 1985 Aug;135(2):1012–1019. [PubMed] [Google Scholar]
- Guesdon J. L., Ternynck T., Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979 Aug;27(8):1131–1139. doi: 10.1177/27.8.90074. [DOI] [PubMed] [Google Scholar]
- Kronenberg M., Siu G., Hood L. E., Shastri N. The molecular genetics of the T-cell antigen receptor and T-cell antigen recognition. Annu Rev Immunol. 1986;4:529–591. doi: 10.1146/annurev.iy.04.040186.002525. [DOI] [PubMed] [Google Scholar]
- Principato M. A., Thompson G. S., Friedman S. M. A cloned major histocompatibility complex-restricted trinitrophenyl-reactive human helper T cell line that activates B cell subsets via two distinct pathways. J Exp Med. 1983 Nov 1;158(5):1444–1458. doi: 10.1084/jem.158.5.1444. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rao A., Ko W. W., Faas S. J., Cantor H. Binding of antigen in the absence of histocompatibility proteins by arsonate-reactive T-cell clones. Cell. 1984 Apr;36(4):879–888. doi: 10.1016/0092-8674(84)90037-0. [DOI] [PubMed] [Google Scholar]
- Schwartz R. H. Immune response (Ir) genes of the murine major histocompatibility complex. Adv Immunol. 1986;38:31–201. doi: 10.1016/s0065-2776(08)60006-1. [DOI] [PubMed] [Google Scholar]
- Thorley-Lawson D. A., Nadler L. M., Bhan A. K., Schooley R. T. BLAST-2 [EBVCS], an early cell surface marker of human B cell activation, is superinduced by Epstein Barr virus. J Immunol. 1985 May;134(5):3007–3012. [PubMed] [Google Scholar]