Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1986 Nov 1;164(5):1749–1759. doi: 10.1084/jem.164.5.1749

Isolation and partial characterization of the reduction-modifiable protein of Neisseria gonorrhoeae

PMCID: PMC2188464  PMID: 3095480

Abstract

We have isolated and purified the reduction-modifiable protein (protein III) from several strains of Neisseria gonorrhoeae. We have found them to be basic proteins (pI 8.6) and virtually identical. A similar but not identical protein was isolated from a meningococcal strain. Antibodies raised against this purified protein would absorb to whole bacteria, confirming that the protein is exposed on the surface of the organism.

Full Text

The Full Text of this article is available as a PDF (638.7 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake M. S., Gotschlich E. C. Purification and partial characterization of the major outer membrane protein of Neisseria gonorrhoeae. Infect Immun. 1982 Apr;36(1):277–283. doi: 10.1128/iai.36.1.277-283.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blake M. S., Gotschlich E. C. Purification and partial characterization of the opacity-associated proteins of Neisseria gonorrhoeae. J Exp Med. 1984 Feb 1;159(2):452–462. doi: 10.1084/jem.159.2.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blake M. S., Gotschlich E. C., Swanson J. Effects of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae. Infect Immun. 1981 Jul;33(1):212–222. doi: 10.1128/iai.33.1.212-222.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jones R. B., Jemison P. A., Newhall W. J., Haak R. A. Resolution of basic gonococcal outer membrane proteins by nonequilibrium pH gradient electrophoresis. Infect Immun. 1980 Dec;30(3):773–780. doi: 10.1128/iai.30.3.773-780.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Judd R. C. 125I-peptide mapping of protein III isolated from four strains of Neisseria gonorrhoeae. Infect Immun. 1982 Aug;37(2):622–631. doi: 10.1128/iai.37.2.622-631.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. King G. J., Swanson J. Studies on gonococcus infection. XV. Identification of surface proteins of Neisseria gonorrhoeae correlated with leukocyte association. Infect Immun. 1978 Aug;21(2):575–584. doi: 10.1128/iai.21.2.575-584.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lynch E. C., Blake M. S., Gotschlich E. C., Mauro A. Studies of Porins: Spontaneously Transferred from Whole Cells and Reconstituted from Purified Proteins of Neisseria gonorrhoeae and Neisseria meningitidis. Biophys J. 1984 Jan;45(1):104–107. doi: 10.1016/S0006-3495(84)84127-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McDade R. L., Jr, Johnston K. H. Characterization of serologically dominant outer membrane proteins of Neisseria gonorrhoeae. J Bacteriol. 1980 Mar;141(3):1183–1191. doi: 10.1128/jb.141.3.1183-1191.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosén A., Ek K., Aman P. Agarose isoelectric focusing of native human immunoglobulin M and alpha 2-macroglobulin. J Immunol Methods. 1979;28(1-2):1–11. doi: 10.1016/0022-1759(79)90322-3. [DOI] [PubMed] [Google Scholar]
  10. Swanson J. Studies on gonococcus infection. XIV. Cell wall protein differences among color/opacity colony variants of Neisseria gonorrhoeae. Infect Immun. 1978 Jul;21(1):292–302. doi: 10.1128/iai.21.1.292-302.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wong T. P., Johnston K. H. One-dimensional peptide mapping of the major outer membrane protein of Neisseria gonorrhoeae. Infect Immun. 1981 Dec;34(3):739–745. doi: 10.1128/iai.34.3.739-745.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Young J. D., Blake M., Mauro A., Cohn Z. A. Properties of the major outer membrane protein from Neisseria gonorrhoeae incorporated into model lipid membranes. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3831–3835. doi: 10.1073/pnas.80.12.3831. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES