Abstract
A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein.
Full Text
The Full Text of this article is available as a PDF (428.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cawley J. C., Barker C. R., Britchford R. D., Smith J. L. Intracellular IgA immunoglobulin crystals in chronic lymphocytic leukaemia. Clin Exp Immunol. 1973 Mar;13(3):407–416. [PMC free article] [PubMed] [Google Scholar]
- Eckhardt A. E., Hayes C. E., Goldstein I. J. A sensitive fluorescent method for the detection of glycoproteins in polyacrylamide gels. Anal Biochem. 1976 May 21;73(1):192–197. doi: 10.1016/0003-2697(76)90154-8. [DOI] [PubMed] [Google Scholar]
- Eulitz M., Linke R. P. Primary structure of the variable part of an amyloidogenic Bence-Jones Protein (Mev.). An unusual insertion in the third hypervariable region of a human kappa-immunoglobulin light chain. Hoppe Seylers Z Physiol Chem. 1982 Nov;363(11):1347–1358. doi: 10.1515/bchm2.1982.363.2.1347. [DOI] [PubMed] [Google Scholar]
- Ferrer-Roca O. Primary gastric plasmacytoma with massive intracytoplasmic crystalline inclusions: a case report. Cancer. 1982 Aug 15;50(4):755–759. doi: 10.1002/1097-0142(19820815)50:4<755::aid-cncr2820500422>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
- Glenner G. G., Ein D., Eanes E. D., Bladen H. A., Terry W., Page D. L. Creation of "amyloid" fibrils from Bence Jones proteins in vitro. Science. 1971 Nov 12;174(4010):712–714. doi: 10.1126/science.174.4010.712. [DOI] [PubMed] [Google Scholar]
- Glenner G. G., Terry W., Harada M., Isersky C., Page D. Amyloid fibril proteins: proof of homology with immunoglobulin light chains by sequence analyses. Science. 1971 Jun 11;172(3988):1150–1151. doi: 10.1126/science.172.3988.1150. [DOI] [PubMed] [Google Scholar]
- Heggeness M. H., Ash J. F. Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy. J Cell Biol. 1977 Jun;73(3):783–788. doi: 10.1083/jcb.73.3.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hickman S., Kulczycki A., Jr, Lynch R. G., Kornfeld S. Studies of the mechanism of tunicamycin in hibition of IgA and IgE secretion by plasma cells. J Biol Chem. 1977 Jun 25;252(12):4402–4408. [PubMed] [Google Scholar]
- Lucas A., Henry C. Expression of the major cross-reactive idiotype in a primary anti-azobenzenearsonate response. J Immunol. 1982 Feb;128(2):802–806. [PubMed] [Google Scholar]
- Miyazaki J., Appella E., Ozato K. Intracellular transport blockade caused by disruption of the disulfide bridge in the third external domain of major histocompatibility complex class I antigen. Proc Natl Acad Sci U S A. 1986 Feb;83(3):757–761. doi: 10.1073/pnas.83.3.757. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakamine H., Nishihara T., Saito K., Takenaka T., Maeda J. Needle-shaped inclusions in plasma cells in a patient with hypogammaglobulinemia. Am J Clin Pathol. 1982 Oct;78(4):549–555. doi: 10.1093/ajcp/78.4.549. [DOI] [PubMed] [Google Scholar]
- Nomura S., Kanoh T., Uchino H. Intracellular formation of amyloid fibrils in myeloma. Cytochemical, immunochemical, and electron microscopic observations. Cancer. 1984 Jul 15;54(2):303–307. doi: 10.1002/1097-0142(19840715)54:2<303::aid-cncr2820540220>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
- Raman S. B., Van Slyck E. J. Nature of intracytoplasmic crystalline inclusions in myeloma cells (morphologic, cytochemical, ultrastructural, and immunofluorescent studies). Am J Clin Pathol. 1983 Aug;80(2):224–228. doi: 10.1093/ajcp/80.2.224. [DOI] [PubMed] [Google Scholar]
- Sletten K., Westermark P., Pitkänen P., Thyresson N., Olstad O. K. Amino acid sequences in amyloid proteins of kappa III immunoglobulin light-chain origin. Scand J Immunol. 1983 Dec;18(6):557–560. doi: 10.1111/j.1365-3083.1983.tb00891.x. [DOI] [PubMed] [Google Scholar]