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. 1987 Apr 1;165(4):959–969. doi: 10.1084/jem.165.4.959

Tyrosine phosphorylation of an interleukin 2 receptor-like protein in cells transformed by human T cell leukemia virus type I

PMCID: PMC2188586  PMID: 2435836

Abstract

Three interleukin 2 (IL-2)-independent human T cell lines transformed with human T cell leukemia virus type I were analyzed for o- phosphotyrosine-containing proteins (Ptyr proteins). Membrane and intracellular immunofluorescence was positive with antibody to Ptyr (Ptyr antibody). 10 size classes of Ptyr proteins with molecular masses of 81-28 kD were isolated with Ptyr antibodies. Among these, proteins of 64 kD (pI 4.5 and 4.8-5.3) and 45 kD (pI 4.3) were found on the outer cell surface. The Ptyr protein of 64 kD (pI 4.5) had the characteristics of the IL-2 receptor (IL-2-R) in that it was recognized by two monoclonal antibodies directed against different epitopes on the IL-2-R.

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Selected References

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  1. FOLEY G. E., LAZARUS H., FARBER S., UZMAN B. G., BOONE B. A., MCCARTHY R. E. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA. Cancer. 1965 Apr;18:522–529. doi: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  2. Hattori T., Uchiyama T., Toibana T., Takatsuki K., Uchino H. Surface phenotype of Japanese adult T-cell leukemia cells characterized by monoclonal antibodies. Blood. 1981 Sep;58(3):645–647. [PubMed] [Google Scholar]
  3. Heldin C. H., Westermark B. Growth factors: mechanism of action and relation to oncogenes. Cell. 1984 May;37(1):9–20. doi: 10.1016/0092-8674(84)90296-4. [DOI] [PubMed] [Google Scholar]
  4. Krueger J. G., Garber E. A., Goldberg A. R. Subcellular localization of pp60src in RSV-transformed cells. Curr Top Microbiol Immunol. 1983;107:51–124. [PubMed] [Google Scholar]
  5. Leonard W. J., Depper J. M., Crabtree G. R., Rudikoff S., Pumphrey J., Robb R. J., Krönke M., Svetlik P. B., Peffer N. J., Waldmann T. A. Molecular cloning and expression of cDNAs for the human interleukin-2 receptor. Nature. 1984 Oct 18;311(5987):626–631. doi: 10.1038/311626a0. [DOI] [PubMed] [Google Scholar]
  6. Leonard W. J., Depper J. M., Krönke M., Robb R. J., Waldmann T. A., Greene W. C. The human receptor for T-cell growth factor. Evidence for variable post-translational processing, phosphorylation, sulfation, and the ability of precursor forms of the receptor to bind T-cell growth factor. J Biol Chem. 1985 Feb 10;260(3):1872–1880. [PubMed] [Google Scholar]
  7. Morikawa S., Tatsumi E., Baba M., Harada T., Yasuhira K. Two E-rosette-forming lymphoid cell lines. Int J Cancer. 1978 Feb 15;21(2):166–170. doi: 10.1002/ijc.2910210207. [DOI] [PubMed] [Google Scholar]
  8. Nikaido T., Shimizu A., Ishida N., Sabe H., Teshigawara K., Maeda M., Uchiyama T., Yodoi J., Honjo T. Molecular cloning of cDNA encoding human interleukin-2 receptor. Nature. 1984 Oct 18;311(5987):631–635. doi: 10.1038/311631a0. [DOI] [PubMed] [Google Scholar]
  9. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  10. Ogawa R., Ohtsuka M., Noguchi K., Iwamura K., Watanabe Y. Anti-O-phosphotyrosine antibodies in human sera. Microbiol Immunol. 1985;29(8):759–767. doi: 10.1111/j.1348-0421.1985.tb00879.x. [DOI] [PubMed] [Google Scholar]
  11. Ogawa R., Ohtsuka M., Sasadaira H., Hirasa M., Yabe H., Uchida H., Watanabe Y. Increase of phosphotyrosine-containing proteins in human carcinomas. Jpn J Cancer Res. 1985 Nov;76(11):1049–1055. [PubMed] [Google Scholar]
  12. Ogawa R., Ohtsuka M., Watanabe Y. Complement-mediated lysis of K562 human leukemic cells by antibodies to phosphotyrosine and identification of cell surface proteins phosphorylated on tyrosine. Cancer Res. 1986 May;46(5):2507–2510. [PubMed] [Google Scholar]
  13. Ogawa R., Ohtsuka M., Watanabe Y., Noguchi K., Arimori S., Sasadaira H. Immunofluorescent staining of human leukemic cells with monoclonal antibody to phosphotyrosine. Jpn J Cancer Res. 1985 Jul;76(7):567–569. [PubMed] [Google Scholar]
  14. Ohtsuka M., Ihara S., Ogawa R., Watanabe T., Watanabe Y. Preparation and characterization of antibodies to O-phosphotyrosine and their use for identification of phosphotyrosine-containing proteins. Int J Cancer. 1984 Dec 15;34(6):855–861. doi: 10.1002/ijc.2910340618. [DOI] [PubMed] [Google Scholar]
  15. Sahai Srivastava B. I., Minowada J. Terminal deoxynucleotidyl transferase activity in a cell line (molt-4) derived from the peripheral blood of a patient with acute lymphoblastic leukemia. Biochem Biophys Res Commun. 1973 Apr 2;51(3):529–535. doi: 10.1016/0006-291x(73)91346-6. [DOI] [PubMed] [Google Scholar]
  16. Shackelford D. A., Trowbridge I. S. Induction of expression and phosphorylation of the human interleukin 2 receptor by a phorbol diester. J Biol Chem. 1984 Oct 10;259(19):11706–11712. [PubMed] [Google Scholar]
  17. Sugamura K., Fujii M., Kannagi M., Sakitani M., Takeuchi M., Hinuma Y. Cell surface phenotypes and expression of viral antigens of various human cell lines carrying human T-cell leukemia virus. Int J Cancer. 1984 Aug 15;34(2):221–228. doi: 10.1002/ijc.2910340213. [DOI] [PubMed] [Google Scholar]
  18. Sugamura K., Nakai S., Fujii M., Hinuma Y. Interleukin 2 inhibits in vitro growth of human T cell lines carrying retrovirus. J Exp Med. 1985 May 1;161(5):1243–1248. doi: 10.1084/jem.161.5.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Swarup G., Cohen S., Garbers D. L. Selective dephosphorylation of proteins containing phosphotyrosine by alkaline phosphatases. J Biol Chem. 1981 Aug 10;256(15):8197–8201. [PubMed] [Google Scholar]
  20. Tanaka Y., Koyanagi Y., Chosa T., Yamamoto N., Hinuma Y. Monoclonal antibody reactive with both p28 and p19 of adult T-cell leukemia virus-specific polypeptides. Gan. 1983 Jun;74(3):327–330. [PubMed] [Google Scholar]
  21. Tanaka Y., Tozawa H., Hayami M., Sugamura K., Hinuma Y. Distinct reactivities of four monoclonal antibodies with human interleukin 2 receptor. Microbiol Immunol. 1985;29(10):959–972. doi: 10.1111/j.1348-0421.1985.tb02960.x. [DOI] [PubMed] [Google Scholar]
  22. Tanaka Y., Tozawa H., Koyanagi Y., Yamamoto N., Hinuma Y. A new monoclonal antibody recognizing an antigen of human lymphocytes similar or identical to Tac antigen. Microbiol Immunol. 1984;28(9):1041–1055. doi: 10.1111/j.1348-0421.1984.tb00760.x. [DOI] [PubMed] [Google Scholar]
  23. Wano Y., Uchiyama T., Fukui K., Maeda M., Uchino H., Yodoi J. Characterization of human interleukin 2 receptor (Tac antigen) in normal and leukemic T cells: co-expression of normal and aberrant receptors on Hut-102 cells. J Immunol. 1984 Jun;132(6):3005–3010. [PubMed] [Google Scholar]

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