Abstract
The acute-phase response to inflammatory stimuli, characterized by increased synthesis of acute-phase proteins (APP), is often accompanied by changes in the glycosylation patterns of some of these proteins. While expression of APP genes in hepatocytes is regulated by monokines, mechanisms governing changes in glycosylation are not known. Exposure of human hepatoma cell line Hep 3B to conditioned medium from LPS- activated human monocytes and to medium from the keratocarcinoma cell line COLO-16 led to increased synthesis of alpha 1 proteinase-inhibitor and ceruloplasmin and to alterations of their glycosylation patterns similar to those seen in human serum in various inflammatory states. IL- 1, tumor necrosis factor, and hepatocyte stimulating factor I increased synthesis of ceruloplasmin without alterations in the pattern of its glycosylation. These findings demonstrate that altered glycosylation seen in plasma in some inflammatory states can be explained by the effects of monokines on glycosylation in hepatocytes and that gene expression and glycosylation of some APP during the acute-phase response may be regulated by different mechanisms.
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- Arend W. P., Massoni R. J. Characteristics of bacterial lipopolysaccharide induction of interleukin 1 synthesis and secretion by human monocytes. Clin Exp Immunol. 1986 Jun;64(3):656–664. [PMC free article] [PubMed] [Google Scholar]
- Baumann H., Hill R. E., Sauder D. N., Jahreis G. P. Regulation of major acute-phase plasma proteins by hepatocyte-stimulating factors of human squamous carcinoma cells. J Cell Biol. 1986 Feb;102(2):370–383. doi: 10.1083/jcb.102.2.370. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Darlington G. J., Wilson D. R., Lachman L. B. Monocyte-conditioned medium, interleukin-1, and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro. J Cell Biol. 1986 Sep;103(3):787–793. doi: 10.1083/jcb.103.3.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Endo M., Suzuki K., Schmid K., Fournet B., Karamanos Y., Montreuil J., Dorland L., van Halbeek H., Vliegenthart J. F. The structures and microheterogeneity of the carbohydrate chains of human plasma ceruloplasmin. A study employing 500-MHz 1H-NMR spectroscopy. J Biol Chem. 1982 Aug 10;257(15):8755–8760. [PubMed] [Google Scholar]
- Koj A., Dubin A., Kasperczyk H., Bereta J., Gordon A. H. Changes in the blood level and affinity to concanavalin A of rat plasma glycoproteins during acute inflammation and hepatoma growth. Biochem J. 1982 Sep 15;206(3):545–553. doi: 10.1042/bj2060545. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kushner I., Mackiewicz A. Acute phase proteins as disease markers. Dis Markers. 1987 Mar;5(1):1–11. [PubMed] [Google Scholar]
- Laurell C. B. Electroimmuno assay. Scand J Clin Lab Invest Suppl. 1972;124:21–37. doi: 10.3109/00365517209102748. [DOI] [PubMed] [Google Scholar]
- Mackiewicz A., Mackiewicz S. Determination of lectin-sugar dissociation constants by agarose affinity electrophoresis. Anal Biochem. 1986 Aug 1;156(2):481–488. doi: 10.1016/0003-2697(86)90282-4. [DOI] [PubMed] [Google Scholar]
- Mackiewicz A., Pawłowski T., Mackiewicz-Pawłowska A., Wiktorowicz K., Mackiewicz S. Microheterogeneity forms of alpha 1-acid glycoprotein as indicators of rheumatoid arthritis activity. Clin Chim Acta. 1987 Mar 16;163(2):185–190. doi: 10.1016/0009-8981(87)90021-0. [DOI] [PubMed] [Google Scholar]
- Mega T., Lujan E., Yoshida A. Studies on the oligosaccharide chains of human alpha 1-protease inhibitor. II. Structure of oligosaccharides. J Biol Chem. 1980 May 10;255(9):4057–4061. [PubMed] [Google Scholar]
- Narasimhan S., Wilson J. R., Martin E., Schachter H. A structural basis for four distinct elution profiles on concanavalin A--Sepharose affinity chromatography of glycopeptides. Can J Biochem. 1979 Jan;57(1):83–96. doi: 10.1139/o79-011. [DOI] [PubMed] [Google Scholar]
- Nicollet I., Lebreton J. P., Fontaine M., Hiron M. Evidence for alpha-1-acid glycoprotein populations of different pI values after concanavalin A affinity chromatography. Study of their evolution during inflammation in man. Biochim Biophys Acta. 1981 Apr 28;668(2):235–245. doi: 10.1016/0005-2795(81)90031-3. [DOI] [PubMed] [Google Scholar]
- Perlmutter D. H., Goldberger G., Dinarello C. A., Mizel S. B., Colten H. R. Regulation of class III major histocompatibility complex gene products by interleukin-1. Science. 1986 May 16;232(4752):850–852. doi: 10.1126/science.3010455. [DOI] [PubMed] [Google Scholar]
- Ritchie D. G., Fuller G. M. Hepatocyte-stimulating factor: a monocyte-derived acute-phase regulatory protein. Ann N Y Acad Sci. 1983 Jun 27;408:490–502. doi: 10.1111/j.1749-6632.1983.tb23268.x. [DOI] [PubMed] [Google Scholar]
- Vaughan L., Lorier M. A., Carrell R. W. alpha 1-Antitrypsin microheterogeneity. Isolation and physiological significance of isoforms. Biochim Biophys Acta. 1982 Mar 4;701(3):339–345. doi: 10.1016/0167-4838(82)90237-0. [DOI] [PubMed] [Google Scholar]
- Woloski B. M., Fuller G. M., Jamieson J. C., Gospodarek E. Studies on the effect of the hepatocyte-stimulating factor on galactose-beta 1----4-N-acetylglucosamine alpha 2----6-sialyltransferase in cultured hepatocytes. Biochim Biophys Acta. 1986 Feb 21;885(2):185–191. doi: 10.1016/0167-4889(86)90087-x. [DOI] [PubMed] [Google Scholar]