Abstract
Mls determinants share with MHC products the unique property of stimulating T cells at extraordinarily high precursor frequencies. The Mls system was originally described as a single locus on chromosome 1, with four alleles, Mlsa, Mlsb, Mlsc, and Mlsd, that encode polymorphic cell surface structures. However, the fundamental issues of polymorphism and allelism in the Mls system remain controversial. To clarify these questions, a formal segregation analysis of the genes encoding Mlsa and Mlsc determinants was carried out by testing the capacity of spleen cells from progeny of (Mlsa X Mlsc)F1 X Mlsb breedings to stimulate responses by unprimed T cells and by Mlsa- and Mlsc-specific cloned T cells. The results of this analysis indicated that the gene encoding Mlsa determinants is neither allelic to nor linked to the gene encoding Mlsc determinants. Together with previous findings, these results also suggest that another strongly stimulatory type, Mlsd, in fact results from the independent expression of unlinked Mlsa and Mlsc gene products. Based on these observations, it is concluded that, contrary to conventional concepts, the stimulatory phenotypes designated as Mlsa, Mlsc, and Mlsd can be accounted for by the independent expression of the products of at least two unlinked gene loci.
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Selected References
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- Abe R., Ryan J. J., Finkelman F. D., Hodes R. J. T cell recognition of Mls. T cell clones demonstrate polymorphism between Mlsa, Mlsc, and Mlsd. J Immunol. 1987 Jan 15;138(2):373–379. [PubMed] [Google Scholar]
- Abe R., Ryan J. J., Hodes R. J. Clonal analysis of the Mls system. A reappraisal of polymorphism and allelism among Mlsa, Mlsc, and Mlsd. J Exp Med. 1987 Apr 1;165(4):1113–1129. doi: 10.1084/jem.165.4.1113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Festenstein H. Immunogenetic and biological aspects of in vitro lymphocyte allotransformation (MLR) in the mouse. Transplant Rev. 1973;15:62–88. doi: 10.1111/j.1600-065x.1973.tb00111.x. [DOI] [PubMed] [Google Scholar]
- Lynch D. H., Gress R. E., Needleman B. W., Rosenberg S. A., Hodes R. J. T cell responses to Mls determinants are restricted by cross-reactive MHC determinants. J Immunol. 1985 Apr;134(4):2071–2078. [PubMed] [Google Scholar]
- Molnar-Kimber K. L., Sprent J. Evidence that strong Mls determinants are nonpolymorphic. Transplantation. 1981 May;31(5):376–378. doi: 10.1097/00007890-198105010-00014. [DOI] [PubMed] [Google Scholar]
- Ryan J. J., Ahmed A., Kind P., Sell K. W. Cross-reactivity exists between Mlsa and Mlsd lymphocyte-activating determinants as demonstrated by the negative clonal selection of responder cells in a mixed lymphocyte reaction. Transplant Proc. 1979 Jun;11(2):1377–1378. [PubMed] [Google Scholar]
- Ryan J. J., Mond J. J., Finkelman F. D., Scher I. Enhancement of the mixed lymphocyte reaction by in vivo treatment of stimulator spleen cells with anti-IgD antibody. J Immunol. 1983 Jun;130(6):2534–2541. [PubMed] [Google Scholar]
- Ryan J. J., Mond J. J., Finkelman F. D. The Mlsd-defined primary mixed lymphocyte reaction: a composite response to Mlsa and Mlsc determinants. J Immunol. 1987 Jun 15;138(12):4085–4092. [PubMed] [Google Scholar]
- Webb S. R., Molnar-Kimber K., Bruce J., Sprent J., Wilson D. B. T cell clones with dual specificity for M1s and various major histocompatibility complex determinants. J Exp Med. 1981 Dec 1;154(6):1970–1974. doi: 10.1084/jem.154.6.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]